The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus
Autophagy is responsible for maintaining fundamental cellular homeostasis and is, therefore, essential for diverse development processes. This study reported that PoElp3, the putative catalytic subunit of Elongator complex, is involved in the maintenance of autophagy homeostasis to facilitate asexua...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-11-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311920634931 |
| _version_ | 1818902963061522432 |
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| author | Li-mei ZHANG Shu-ting CHEN Min QI Xue-qi CAO Nan LIANG Qian LI Wei TANG Guo-dong LU Jie ZHOU Wen-ying YU Zong-hua WANG Hua-kun ZHENG |
| author_facet | Li-mei ZHANG Shu-ting CHEN Min QI Xue-qi CAO Nan LIANG Qian LI Wei TANG Guo-dong LU Jie ZHOU Wen-ying YU Zong-hua WANG Hua-kun ZHENG |
| author_sort | Li-mei ZHANG |
| collection | DOAJ |
| description | Autophagy is responsible for maintaining fundamental cellular homeostasis and is, therefore, essential for diverse development processes. This study reported that PoElp3, the putative catalytic subunit of Elongator complex, is involved in the maintenance of autophagy homeostasis to facilitate asexual development and pathogenicity in the rice blast fungus Pyricularia oryzae. It was found that the ΔPoelp3 strains were defective in vegetative growth, conidiation, stress response, and pathogenicity. The mutants exhibited hyper-activated autophagy in the vegetative hyphae under both nutrient-rich and nutrient-deficient conditions. The hyper-activation of autophagy possibly suppressed the production of vegetative hyphae in the ΔPoelp3 strains. Moreover, the ΔPoelp3 strains were found to be more sensitive to rapamycin during vegetative- and invasive-hyphal growth but have no effect on Target-of-Rapamycin (TOR) signaling inhibition. Taken together, these results demonstrated that PoElp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus. |
| first_indexed | 2024-12-19T20:43:59Z |
| format | Article |
| id | doaj.art-2fcaf37663cf41b7957b6e2194b495ff |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-19T20:43:59Z |
| publishDate | 2021-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-2fcaf37663cf41b7957b6e2194b495ff2022-12-21T20:06:19ZengElsevierJournal of Integrative Agriculture2095-31192021-11-01201129442956The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungusLi-mei ZHANG0Shu-ting CHEN1Min QI2Xue-qi CAO3Nan LIANG4Qian LI5Wei TANG6Guo-dong LU7Jie ZHOU8Wen-ying YU9Zong-hua WANG10Hua-kun ZHENG11State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China; Fujian Universities Key Laboratory for Plant–Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaMeishan Vocational and Technical College, Meishan 620010, P.R.ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China; Fujian Universities Key Laboratory for Plant–Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China; Fujian Universities Key Laboratory for Plant–Microbe Interaction, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China; Correspondence YU Wen-yingState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China; Institute of Oceanography, Minjiang University, Fuzhou 350108, P.R.China; Correspondence WANG Zong-huaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China; National Engineering Research Center of JUNCAO Technology, College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R.China; Correspondence ZHENG Hua-kunAutophagy is responsible for maintaining fundamental cellular homeostasis and is, therefore, essential for diverse development processes. This study reported that PoElp3, the putative catalytic subunit of Elongator complex, is involved in the maintenance of autophagy homeostasis to facilitate asexual development and pathogenicity in the rice blast fungus Pyricularia oryzae. It was found that the ΔPoelp3 strains were defective in vegetative growth, conidiation, stress response, and pathogenicity. The mutants exhibited hyper-activated autophagy in the vegetative hyphae under both nutrient-rich and nutrient-deficient conditions. The hyper-activation of autophagy possibly suppressed the production of vegetative hyphae in the ΔPoelp3 strains. Moreover, the ΔPoelp3 strains were found to be more sensitive to rapamycin during vegetative- and invasive-hyphal growth but have no effect on Target-of-Rapamycin (TOR) signaling inhibition. Taken together, these results demonstrated that PoElp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus.http://www.sciencedirect.com/science/article/pii/S2095311920634931elongatorElp3autophagyasexual developmentpathogenicityPyricularia oryzae |
| spellingShingle | Li-mei ZHANG Shu-ting CHEN Min QI Xue-qi CAO Nan LIANG Qian LI Wei TANG Guo-dong LU Jie ZHOU Wen-ying YU Zong-hua WANG Hua-kun ZHENG The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus Journal of Integrative Agriculture elongator Elp3 autophagy asexual development pathogenicity Pyricularia oryzae |
| title | The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus |
| title_full | The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus |
| title_fullStr | The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus |
| title_full_unstemmed | The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus |
| title_short | The putative elongator complex protein Elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus |
| title_sort | putative elongator complex protein elp3 is involved in asexual development and pathogenicity by regulating autophagy in the rice blast fungus |
| topic | elongator Elp3 autophagy asexual development pathogenicity Pyricularia oryzae |
| url | http://www.sciencedirect.com/science/article/pii/S2095311920634931 |
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