Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production
Abstract Backgrounds Engineering yeast as a consolidated bioprocessing (CBP) microorganism by surface assembly of cellulosomes has been aggressively utilized for cellulosic ethanol production. However, most of the previous studies focused on Saccharomyces cerevisiae, achieving efficient conversion o...
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| Format: | Article |
| Language: | English |
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BMC
2020-06-01
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| Series: | Biotechnology for Biofuels |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13068-020-01749-1 |
| _version_ | 1828335973712265216 |
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| author | Ce Dong Jie Qiao Xinping Wang Wenli Sun Lixia Chen Shuntang Li Ke Wu Lixin Ma Yi Liu |
| author_facet | Ce Dong Jie Qiao Xinping Wang Wenli Sun Lixia Chen Shuntang Li Ke Wu Lixin Ma Yi Liu |
| author_sort | Ce Dong |
| collection | DOAJ |
| description | Abstract Backgrounds Engineering yeast as a consolidated bioprocessing (CBP) microorganism by surface assembly of cellulosomes has been aggressively utilized for cellulosic ethanol production. However, most of the previous studies focused on Saccharomyces cerevisiae, achieving efficient conversion of phosphoric acid-swollen cellulose (PASC) or microcrystalline cellulose (Avicel) but not carboxymethyl cellulose (CMC) to ethanol, with an average titer below 2 g/L. Results Harnessing an ultra-high-affinity IM7/CL7 protein pair, here we describe a method to engineer Pichia pastoris with minicellulosomes by in vitro assembly of three recombinant cellulases including an endoglucanase (EG), an exoglucanase (CBH) and a β-glucosidase (BGL), as well as a carbohydrate-binding module (CBM) on the cell surface. For the first time, the engineered yeasts enable efficient and direct conversion of CMC to bioethanol, observing an impressive ethanol titer of 5.1 g/L. Conclusions The research promotes the application of P. pastoris as a CBP cell factory in cellulosic ethanol production and provides a promising platform for screening the cellulases from different species to construct surface-assembly celluosome. |
| first_indexed | 2024-04-13T21:54:20Z |
| format | Article |
| id | doaj.art-2fcd15bada914515a493ab5ee5d3a0e0 |
| institution | Directory Open Access Journal |
| issn | 1754-6834 |
| language | English |
| last_indexed | 2024-04-13T21:54:20Z |
| publishDate | 2020-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Biotechnology for Biofuels |
| spelling | doaj.art-2fcd15bada914515a493ab5ee5d3a0e02022-12-22T02:28:19ZengBMCBiotechnology for Biofuels1754-68342020-06-011311910.1186/s13068-020-01749-1Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol productionCe Dong0Jie Qiao1Xinping Wang2Wenli Sun3Lixia Chen4Shuntang Li5Ke Wu6Lixin Ma7Yi Liu8State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityHubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityState Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei UniversityAbstract Backgrounds Engineering yeast as a consolidated bioprocessing (CBP) microorganism by surface assembly of cellulosomes has been aggressively utilized for cellulosic ethanol production. However, most of the previous studies focused on Saccharomyces cerevisiae, achieving efficient conversion of phosphoric acid-swollen cellulose (PASC) or microcrystalline cellulose (Avicel) but not carboxymethyl cellulose (CMC) to ethanol, with an average titer below 2 g/L. Results Harnessing an ultra-high-affinity IM7/CL7 protein pair, here we describe a method to engineer Pichia pastoris with minicellulosomes by in vitro assembly of three recombinant cellulases including an endoglucanase (EG), an exoglucanase (CBH) and a β-glucosidase (BGL), as well as a carbohydrate-binding module (CBM) on the cell surface. For the first time, the engineered yeasts enable efficient and direct conversion of CMC to bioethanol, observing an impressive ethanol titer of 5.1 g/L. Conclusions The research promotes the application of P. pastoris as a CBP cell factory in cellulosic ethanol production and provides a promising platform for screening the cellulases from different species to construct surface-assembly celluosome.http://link.springer.com/article/10.1186/s13068-020-01749-1CellulosomePichia pastorisConsolidate bioprocessing (CBP)Carboxymethyl cellulose (CMC)Bioethanol |
| spellingShingle | Ce Dong Jie Qiao Xinping Wang Wenli Sun Lixia Chen Shuntang Li Ke Wu Lixin Ma Yi Liu Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production Biotechnology for Biofuels Cellulosome Pichia pastoris Consolidate bioprocessing (CBP) Carboxymethyl cellulose (CMC) Bioethanol |
| title | Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production |
| title_full | Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production |
| title_fullStr | Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production |
| title_full_unstemmed | Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production |
| title_short | Engineering Pichia pastoris with surface-display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production |
| title_sort | engineering pichia pastoris with surface display minicellulosomes for carboxymethyl cellulose hydrolysis and ethanol production |
| topic | Cellulosome Pichia pastoris Consolidate bioprocessing (CBP) Carboxymethyl cellulose (CMC) Bioethanol |
| url | http://link.springer.com/article/10.1186/s13068-020-01749-1 |
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