Long-Term Preservation of Rat Skin Tissue by Epigallocatechin-3--Gallate
Skin grafts can be preserved by cryopreservation and refrigerated storage at 4°C. Epigallocatechin-3- O -gallate (EGCG) enhances the viability of stored skin grafts and also extends the storage time up to 7 weeks at 4°C. EGCG, the major polyphenolic constituent present in green tea, has potent antio...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2009-05-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/096368970901805-605 |
| _version_ | 1829106031082340352 |
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| author | Hakhee Kim Takeshi Kawazoe Kazuaki Matsumura Shigehiko Suzuki Suong-Hyu Hyon Ph.D. |
| author_facet | Hakhee Kim Takeshi Kawazoe Kazuaki Matsumura Shigehiko Suzuki Suong-Hyu Hyon Ph.D. |
| author_sort | Hakhee Kim |
| collection | DOAJ |
| description | Skin grafts can be preserved by cryopreservation and refrigerated storage at 4°C. Epigallocatechin-3- O -gallate (EGCG) enhances the viability of stored skin grafts and also extends the storage time up to 7 weeks at 4°C. EGCG, the major polyphenolic constituent present in green tea, has potent antioxidant, antimicrobial, antiproliferative, and free radical scavenging effects. This study examined the effects of EGCG on skin cryopreservation. Skin sample biopsy specimens from GFP rats were previously treated with/without EGCG then moved to −196°C. Skin samples were transplanted to nude mice after 2, 8, and 24 weeks of preservation. Glucose consumption was measured after thawing to assess the metabolic activity. Two weeks later the transplanted skin grafts were excised and histologically analyzed. Histological examinations revealed the degeneration of the epidermal and dermal layers in all groups. In the EGCG groups, the grafts showed higher integrity in the epidermal layer and dermal matrix. The present findings suggest the future clinical usefulness of EGCG for skin preservation; however, the mechanism by which EGCG promotes skin preservation still remains unclear. |
| first_indexed | 2024-12-12T06:16:56Z |
| format | Article |
| id | doaj.art-2fd06f9216f140c8ba53e458a3751fb4 |
| institution | Directory Open Access Journal |
| issn | 0963-6897 1555-3892 |
| language | English |
| last_indexed | 2024-12-12T06:16:56Z |
| publishDate | 2009-05-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj.art-2fd06f9216f140c8ba53e458a3751fb42022-12-22T00:35:00ZengSAGE PublishingCell Transplantation0963-68971555-38922009-05-011810.1177/096368970901805-605Long-Term Preservation of Rat Skin Tissue by Epigallocatechin-3--GallateHakhee Kim0Takeshi Kawazoe1Kazuaki Matsumura2Shigehiko Suzuki3Suong-Hyu Hyon Ph.D.4Department of Medical Simulation Engineering, Research Center for Nano Medical Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, JapanDepartment of Plastic and Reconstructive Surgery, Kijunkai, Yoshikawa Hospital, Kyoto 606-8392, JapanDepartment of Medical Simulation Engineering, Research Center for Nano Medical Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, JapanDepartment of Plastic and Reconstructive Surgery, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, JapanDepartment of Medical Simulation Engineering, Research Center for Nano Medical Engineering, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, JapanSkin grafts can be preserved by cryopreservation and refrigerated storage at 4°C. Epigallocatechin-3- O -gallate (EGCG) enhances the viability of stored skin grafts and also extends the storage time up to 7 weeks at 4°C. EGCG, the major polyphenolic constituent present in green tea, has potent antioxidant, antimicrobial, antiproliferative, and free radical scavenging effects. This study examined the effects of EGCG on skin cryopreservation. Skin sample biopsy specimens from GFP rats were previously treated with/without EGCG then moved to −196°C. Skin samples were transplanted to nude mice after 2, 8, and 24 weeks of preservation. Glucose consumption was measured after thawing to assess the metabolic activity. Two weeks later the transplanted skin grafts were excised and histologically analyzed. Histological examinations revealed the degeneration of the epidermal and dermal layers in all groups. In the EGCG groups, the grafts showed higher integrity in the epidermal layer and dermal matrix. The present findings suggest the future clinical usefulness of EGCG for skin preservation; however, the mechanism by which EGCG promotes skin preservation still remains unclear.https://doi.org/10.1177/096368970901805-605 |
| spellingShingle | Hakhee Kim Takeshi Kawazoe Kazuaki Matsumura Shigehiko Suzuki Suong-Hyu Hyon Ph.D. Long-Term Preservation of Rat Skin Tissue by Epigallocatechin-3--Gallate Cell Transplantation |
| title | Long-Term Preservation of Rat Skin Tissue by Epigallocatechin-3--Gallate |
| title_full | Long-Term Preservation of Rat Skin Tissue by Epigallocatechin-3--Gallate |
| title_fullStr | Long-Term Preservation of Rat Skin Tissue by Epigallocatechin-3--Gallate |
| title_full_unstemmed | Long-Term Preservation of Rat Skin Tissue by Epigallocatechin-3--Gallate |
| title_short | Long-Term Preservation of Rat Skin Tissue by Epigallocatechin-3--Gallate |
| title_sort | long term preservation of rat skin tissue by epigallocatechin 3 gallate |
| url | https://doi.org/10.1177/096368970901805-605 |
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