Phylogeny of <em>Anopheles</em> (<em>Kerteszia</em>) (Diptera: Culicidae) Using Mitochondrial Genes
Identification of mosquito species is necessary for determining the entomological components of malaria transmission, but it can be difficult in morphologically similar species. DNA sequences are largely used as an additional tool for species recognition, including those that belong to species compl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/11/5/324 |
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| author | Karin Kirchgatter Lilian de Oliveira Guimarães Henrry Hugo Yañez Trujillano Fernando Rafael Arias Abraham Germán Cáceres Ana Maria Ribeiro de Castro Duarte Rosely dos Santos Malafronte Rosa Maria Tubaki Maria Anice Mureb Sallum |
| author_facet | Karin Kirchgatter Lilian de Oliveira Guimarães Henrry Hugo Yañez Trujillano Fernando Rafael Arias Abraham Germán Cáceres Ana Maria Ribeiro de Castro Duarte Rosely dos Santos Malafronte Rosa Maria Tubaki Maria Anice Mureb Sallum |
| author_sort | Karin Kirchgatter |
| collection | DOAJ |
| description | Identification of mosquito species is necessary for determining the entomological components of malaria transmission, but it can be difficult in morphologically similar species. DNA sequences are largely used as an additional tool for species recognition, including those that belong to species complexes. <i>Kerteszia</i> mosquitoes are vectors of human and simian malaria in the Neotropical Region, but there are few DNA sequences of <i>Kerteszia</i> species in public databases. In order to provide relevant information about diversity and improve knowledge in taxonomy of <i>Kerteszia</i> species in Peru, we sequenced part of the mitochondrial genome, including the cytochrome c oxidase I (COI) barcode region. Phylogenetic analyses structured all species of mosquitoes collected in Peru into a single clade, separate from the Brazilian species. The Peruvian clade was composed of two lineages, encompassing sequences from <i>Anopheles (Kerteszia) boliviensis</i> and <i>Anopheles (Kerteszia) pholidotus</i>. <i>An. pholidotus</i> sequences were recorded for the first time in Peru, whereas <i>An. boliviensis</i> sequences were for the first time published in the GenBank database. Sequences generated from specimens morphologically identified as <i>Anopheles (Kerteszia) cruzii</i> clustered into three separate clades according to the collection localities of Serra do Mar, Serra da Mantiqueira, and Serra da Cantareira, confirming <i>An. cruzii</i> as a species complex, composed of at least three putative species. |
| first_indexed | 2024-03-10T19:37:27Z |
| format | Article |
| id | doaj.art-2fd2540425764b50afbce20825d04710 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T19:37:27Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-2fd2540425764b50afbce20825d047102023-11-20T01:34:03ZengMDPI AGInsects2075-44502020-05-0111532410.3390/insects11050324Phylogeny of <em>Anopheles</em> (<em>Kerteszia</em>) (Diptera: Culicidae) Using Mitochondrial GenesKarin Kirchgatter0Lilian de Oliveira Guimarães1Henrry Hugo Yañez Trujillano2Fernando Rafael Arias3Abraham Germán Cáceres4Ana Maria Ribeiro de Castro Duarte5Rosely dos Santos Malafronte6Rosa Maria Tubaki7Maria Anice Mureb Sallum8Laboratório de Bioquímica e Biologia Molecular, Superintendência de Controle de Endemias/Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, SP 01027-000, BrazilLaboratório de Bioquímica e Biologia Molecular, Superintendência de Controle de Endemias/Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, SP 01027-000, BrazilArea de Vigilancia y Control Vectorial, Dirección de Salud Ambiental, Dirección Ejecutiva de Salud Ambiental, Dirección Regional de Salud Cusco, Cusco 08003, PeruLaboratorio de Referencia Regional de Salud Pública, Dirección Regional de Salud Puno, Puno 21002, PeruSeccion de Entomología, Instituto de Medicina Tropical “Daniel A. Carrión” y Departamento Académico de Microbiología Médica, Facultad de Medicina, Universidad Nacional Mayor de San Marcos, Lima 15081, PeruLaboratório de Bioquímica e Biologia Molecular, Superintendência de Controle de Endemias/Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, SP 01027-000, BrazilLaboratório de Protozoologia, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, SP 05403-000, BrazilLaboratório de Entomologia Médica, Superintendência de Controle de Endemias, São Paulo, SP 01027-000, BrazilDepartamento de Epidemiologia, Faculdade de Saúde Pública, Universidade de São Paulo, São Paulo, SP 01246-904, BrazilIdentification of mosquito species is necessary for determining the entomological components of malaria transmission, but it can be difficult in morphologically similar species. DNA sequences are largely used as an additional tool for species recognition, including those that belong to species complexes. <i>Kerteszia</i> mosquitoes are vectors of human and simian malaria in the Neotropical Region, but there are few DNA sequences of <i>Kerteszia</i> species in public databases. In order to provide relevant information about diversity and improve knowledge in taxonomy of <i>Kerteszia</i> species in Peru, we sequenced part of the mitochondrial genome, including the cytochrome c oxidase I (COI) barcode region. Phylogenetic analyses structured all species of mosquitoes collected in Peru into a single clade, separate from the Brazilian species. The Peruvian clade was composed of two lineages, encompassing sequences from <i>Anopheles (Kerteszia) boliviensis</i> and <i>Anopheles (Kerteszia) pholidotus</i>. <i>An. pholidotus</i> sequences were recorded for the first time in Peru, whereas <i>An. boliviensis</i> sequences were for the first time published in the GenBank database. Sequences generated from specimens morphologically identified as <i>Anopheles (Kerteszia) cruzii</i> clustered into three separate clades according to the collection localities of Serra do Mar, Serra da Mantiqueira, and Serra da Cantareira, confirming <i>An. cruzii</i> as a species complex, composed of at least three putative species.https://www.mdpi.com/2075-4450/11/5/324mosquitoesmalaria<i>Kerteszia</i>Perupopulation geneticsbarcoding |
| spellingShingle | Karin Kirchgatter Lilian de Oliveira Guimarães Henrry Hugo Yañez Trujillano Fernando Rafael Arias Abraham Germán Cáceres Ana Maria Ribeiro de Castro Duarte Rosely dos Santos Malafronte Rosa Maria Tubaki Maria Anice Mureb Sallum Phylogeny of <em>Anopheles</em> (<em>Kerteszia</em>) (Diptera: Culicidae) Using Mitochondrial Genes Insects mosquitoes malaria <i>Kerteszia</i> Peru population genetics barcoding |
| title | Phylogeny of <em>Anopheles</em> (<em>Kerteszia</em>) (Diptera: Culicidae) Using Mitochondrial Genes |
| title_full | Phylogeny of <em>Anopheles</em> (<em>Kerteszia</em>) (Diptera: Culicidae) Using Mitochondrial Genes |
| title_fullStr | Phylogeny of <em>Anopheles</em> (<em>Kerteszia</em>) (Diptera: Culicidae) Using Mitochondrial Genes |
| title_full_unstemmed | Phylogeny of <em>Anopheles</em> (<em>Kerteszia</em>) (Diptera: Culicidae) Using Mitochondrial Genes |
| title_short | Phylogeny of <em>Anopheles</em> (<em>Kerteszia</em>) (Diptera: Culicidae) Using Mitochondrial Genes |
| title_sort | phylogeny of em anopheles em em kerteszia em diptera culicidae using mitochondrial genes |
| topic | mosquitoes malaria <i>Kerteszia</i> Peru population genetics barcoding |
| url | https://www.mdpi.com/2075-4450/11/5/324 |
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