Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s Study
Prenatal exposure to methylmercury (MeHg) affects child development after birth. However, many epidemiological studies have evaluated total mercury levels without analyzing speciation. Biomonitoring of MeHg and inorganic mercury (IHg) is essential to reveal each exposure level. In this study, we com...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Toxics |
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| Online Access: | https://www.mdpi.com/2305-6304/9/4/82 |
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| author | Miyuki Iwai-Shimada Yayoi Kobayashi Tomohiko Isobe Shoji F. Nakayama Makiko Sekiyama Yu Taniguchi Shin Yamazaki Takehiro Michikawa Masako Oda Hiroshi Mitsubuchi Masafumi Sanefuji Shouichi Ohga Nathan Mise Akihiko Ikegami Reiko Suga Masayuki Shimono |
| author_facet | Miyuki Iwai-Shimada Yayoi Kobayashi Tomohiko Isobe Shoji F. Nakayama Makiko Sekiyama Yu Taniguchi Shin Yamazaki Takehiro Michikawa Masako Oda Hiroshi Mitsubuchi Masafumi Sanefuji Shouichi Ohga Nathan Mise Akihiko Ikegami Reiko Suga Masayuki Shimono |
| author_sort | Miyuki Iwai-Shimada |
| collection | DOAJ |
| description | Prenatal exposure to methylmercury (MeHg) affects child development after birth. However, many epidemiological studies have evaluated total mercury levels without analyzing speciation. Biomonitoring of MeHg and inorganic mercury (IHg) is essential to reveal each exposure level. In this study, we compared a high-throughput analysis for mercury speciation in blood using liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) and liquid chromatography-cold vapor atomic fluorescence spectrometry (LC-CVAFS). The validated LC-ICP-MS method was applied to 101 maternal blood and 366 cord blood samples in the pilot study of the Japan Environment and Children’s Study (JECS). The accuracy of the LC-CVAFS method ranged 90–115% determined by reference material analysis. To evaluate the reliability of 366 cord blood samples, fifty cord blood samples were randomly selected and analyzed using LC-CVAFS. The median (5th–95th percentile) concentrations of MeHg and IHg were 5.4 (1.9–15) and 0.33 (0.12–0.86) ng/mL, respectively, in maternal blood, and 6.3 (2.5–15) and 0.21 (0.08–0.49) ng/mL, respectively, in cord blood. Inter-laboratory comparison showed a relatively good agreement between LC-ICP-MS and LC-CVAFS. The median cord blood:maternal blood ratios of MeHg and IHg were 1.3 and 0.5, respectively. By analyzing speciation, we could focus on the health effects of each chemical form. |
| first_indexed | 2024-03-10T12:27:48Z |
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| id | doaj.art-2fd30136b3a246eb92ae06d06ba99285 |
| institution | Directory Open Access Journal |
| issn | 2305-6304 |
| language | English |
| last_indexed | 2024-03-10T12:27:48Z |
| publishDate | 2021-04-01 |
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| series | Toxics |
| spelling | doaj.art-2fd30136b3a246eb92ae06d06ba992852023-11-21T14:55:01ZengMDPI AGToxics2305-63042021-04-01948210.3390/toxics9040082Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s StudyMiyuki Iwai-Shimada0Yayoi Kobayashi1Tomohiko Isobe2Shoji F. Nakayama3Makiko Sekiyama4Yu Taniguchi5Shin Yamazaki6Takehiro Michikawa7Masako Oda8Hiroshi Mitsubuchi9Masafumi Sanefuji10Shouichi Ohga11Nathan Mise12Akihiko Ikegami13Reiko Suga14Masayuki Shimono15Japan Environment and Children’s Study Programme Office, National Institute for Environmental Studies, Tsukuba 305-8506, JapanJapan Environment and Children’s Study Programme Office, National Institute for Environmental Studies, Tsukuba 305-8506, JapanJapan Environment and Children’s Study Programme Office, National Institute for Environmental Studies, Tsukuba 305-8506, JapanJapan Environment and Children’s Study Programme Office, National Institute for Environmental Studies, Tsukuba 305-8506, JapanJapan Environment and Children’s Study Programme Office, National Institute for Environmental Studies, Tsukuba 305-8506, JapanJapan Environment and Children’s Study Programme Office, National Institute for Environmental Studies, Tsukuba 305-8506, JapanJapan Environment and Children’s Study Programme Office, National Institute for Environmental Studies, Tsukuba 305-8506, JapanDepartment of Environmental and Occupational Health, School of Medicine, Toho University, Tokyo 143-8540, JapanThe Southern Kyusyu and Okinawa Regional Centre, Faculty of Life Sciences, Kumamoto University, Kumamoto 860-8556, JapanDepartment of Neonatology, Kumamoto University Hospital, Kumamoto 860-8556, JapanResearch Center for Environment and Developmental Medical Sciences, Kyushu University, Fukuoka 812-8582, JapanResearch Center for Environment and Developmental Medical Sciences, Kyushu University, Fukuoka 812-8582, JapanDepartment of Environmental and Preventive Medicine, Jichi Medical University, Tochigi 329-0498, JapanDepartment of Environmental and Preventive Medicine, Jichi Medical University, Tochigi 329-0498, JapanRegional Center for Japan Environment and Children’s Study, University of Occupational and Environmental Health, Kitakyushu 807-8555, JapanRegional Center for Japan Environment and Children’s Study, University of Occupational and Environmental Health, Kitakyushu 807-8555, JapanPrenatal exposure to methylmercury (MeHg) affects child development after birth. However, many epidemiological studies have evaluated total mercury levels without analyzing speciation. Biomonitoring of MeHg and inorganic mercury (IHg) is essential to reveal each exposure level. In this study, we compared a high-throughput analysis for mercury speciation in blood using liquid chromatography-inductively coupled plasma-mass spectrometry (LC-ICP-MS) and liquid chromatography-cold vapor atomic fluorescence spectrometry (LC-CVAFS). The validated LC-ICP-MS method was applied to 101 maternal blood and 366 cord blood samples in the pilot study of the Japan Environment and Children’s Study (JECS). The accuracy of the LC-CVAFS method ranged 90–115% determined by reference material analysis. To evaluate the reliability of 366 cord blood samples, fifty cord blood samples were randomly selected and analyzed using LC-CVAFS. The median (5th–95th percentile) concentrations of MeHg and IHg were 5.4 (1.9–15) and 0.33 (0.12–0.86) ng/mL, respectively, in maternal blood, and 6.3 (2.5–15) and 0.21 (0.08–0.49) ng/mL, respectively, in cord blood. Inter-laboratory comparison showed a relatively good agreement between LC-ICP-MS and LC-CVAFS. The median cord blood:maternal blood ratios of MeHg and IHg were 1.3 and 0.5, respectively. By analyzing speciation, we could focus on the health effects of each chemical form.https://www.mdpi.com/2305-6304/9/4/82mercurymethylmercuryinorganic mercurymercury speciationcohort studyblood |
| spellingShingle | Miyuki Iwai-Shimada Yayoi Kobayashi Tomohiko Isobe Shoji F. Nakayama Makiko Sekiyama Yu Taniguchi Shin Yamazaki Takehiro Michikawa Masako Oda Hiroshi Mitsubuchi Masafumi Sanefuji Shouichi Ohga Nathan Mise Akihiko Ikegami Reiko Suga Masayuki Shimono Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s Study Toxics mercury methylmercury inorganic mercury mercury speciation cohort study blood |
| title | Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s Study |
| title_full | Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s Study |
| title_fullStr | Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s Study |
| title_full_unstemmed | Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s Study |
| title_short | Comparison of Simultaneous Quantitative Analysis of Methylmercury and Inorganic Mercury in Cord Blood Using LC-ICP-MS and LC-CVAFS: The Pilot Study of the Japan Environment and Children’s Study |
| title_sort | comparison of simultaneous quantitative analysis of methylmercury and inorganic mercury in cord blood using lc icp ms and lc cvafs the pilot study of the japan environment and children s study |
| topic | mercury methylmercury inorganic mercury mercury speciation cohort study blood |
| url | https://www.mdpi.com/2305-6304/9/4/82 |
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