2,5-Diketo-D-Gluconate Hyperproducing <i>Gluconobacter sphaericus</i> SJF2-1 with Reporting Multiple Genes Encoding the Membrane-Associated Flavoprotein-Cytochrome c Complexed Dehydrogenases
<i>Gluconobacter sphaericus</i> has not yet been used in biotransformation studies. In this study, <i>G. sphaericus</i> SJF2-1, which produces a diffusible pigment, was isolated from grape. The spent culture medium became dark black when the cells were grown in medium contain...
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MDPI AG
2022-10-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/10/11/2130 |
| _version_ | 1827645983793610752 |
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| author | Haelim Son Sang-Uk Han Kyoung Lee |
| author_facet | Haelim Son Sang-Uk Han Kyoung Lee |
| author_sort | Haelim Son |
| collection | DOAJ |
| description | <i>Gluconobacter sphaericus</i> has not yet been used in biotransformation studies. In this study, <i>G. sphaericus</i> SJF2-1, which produces a diffusible pigment, was isolated from grape. The spent culture medium became dark black when the cells were grown in medium containing glucose and then autoclaved. This bacterium produced 2,5-diketo-D-gluconate (2,5-DKG) from D-glucose and D-gluconate. When 5% D-glucose was used, the conversion efficiency was approximately 52.4% in a flask culture. 2,5-DKG is a precursor of 2-keto-L-gulonic acid, which is a key intermediate in the industrial production of L-ascorbic acid. The complete genome sequence of <i>G. sphaericus</i> SJF2-1 was determined for the first time in the <i>G. sphaericus</i> species. The total size was 3,198,086 bp, with 2867 protein-coding sequences; one chromosome and six plasmids were identified. From the genome of SJF2-1, multiple genes homologous to those involved in the conversion of D-glucose to 2,5-DKG were identified. In particular, six different genes encoding membrane-associated flavoprotein-cytochrome c complexed dehydrogenase were identified and divided into two different lineages. This study suggests the potential of <i>G. sphaericus</i> SJF2-1 to mass-produce 2,5-DKG and other D-glucose oxidation products. |
| first_indexed | 2024-03-09T18:49:43Z |
| format | Article |
| id | doaj.art-2fd3b9994b924826a7caec7bdd3ab8e9 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T18:49:43Z |
| publishDate | 2022-10-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-2fd3b9994b924826a7caec7bdd3ab8e92023-11-24T05:56:16ZengMDPI AGMicroorganisms2076-26072022-10-011011213010.3390/microorganisms101121302,5-Diketo-D-Gluconate Hyperproducing <i>Gluconobacter sphaericus</i> SJF2-1 with Reporting Multiple Genes Encoding the Membrane-Associated Flavoprotein-Cytochrome c Complexed DehydrogenasesHaelim Son0Sang-Uk Han1Kyoung Lee2Department of Bio Health Science, Changwon National University, Changwon 51140, KoreaDepartment of Bio Health Science, Changwon National University, Changwon 51140, KoreaDepartment of Bio Health Science, Changwon National University, Changwon 51140, Korea<i>Gluconobacter sphaericus</i> has not yet been used in biotransformation studies. In this study, <i>G. sphaericus</i> SJF2-1, which produces a diffusible pigment, was isolated from grape. The spent culture medium became dark black when the cells were grown in medium containing glucose and then autoclaved. This bacterium produced 2,5-diketo-D-gluconate (2,5-DKG) from D-glucose and D-gluconate. When 5% D-glucose was used, the conversion efficiency was approximately 52.4% in a flask culture. 2,5-DKG is a precursor of 2-keto-L-gulonic acid, which is a key intermediate in the industrial production of L-ascorbic acid. The complete genome sequence of <i>G. sphaericus</i> SJF2-1 was determined for the first time in the <i>G. sphaericus</i> species. The total size was 3,198,086 bp, with 2867 protein-coding sequences; one chromosome and six plasmids were identified. From the genome of SJF2-1, multiple genes homologous to those involved in the conversion of D-glucose to 2,5-DKG were identified. In particular, six different genes encoding membrane-associated flavoprotein-cytochrome c complexed dehydrogenase were identified and divided into two different lineages. This study suggests the potential of <i>G. sphaericus</i> SJF2-1 to mass-produce 2,5-DKG and other D-glucose oxidation products.https://www.mdpi.com/2076-2607/10/11/2130<i>Gluconobacter sphaericus</i>2,5-diketo-D-gluconatemembrane-associated flavoprotein-cytochrome c complexed dehydrogenaseL-ascorbic acidD-glucose biotransformationD-gluconate |
| spellingShingle | Haelim Son Sang-Uk Han Kyoung Lee 2,5-Diketo-D-Gluconate Hyperproducing <i>Gluconobacter sphaericus</i> SJF2-1 with Reporting Multiple Genes Encoding the Membrane-Associated Flavoprotein-Cytochrome c Complexed Dehydrogenases Microorganisms <i>Gluconobacter sphaericus</i> 2,5-diketo-D-gluconate membrane-associated flavoprotein-cytochrome c complexed dehydrogenase L-ascorbic acid D-glucose biotransformation D-gluconate |
| title | 2,5-Diketo-D-Gluconate Hyperproducing <i>Gluconobacter sphaericus</i> SJF2-1 with Reporting Multiple Genes Encoding the Membrane-Associated Flavoprotein-Cytochrome c Complexed Dehydrogenases |
| title_full | 2,5-Diketo-D-Gluconate Hyperproducing <i>Gluconobacter sphaericus</i> SJF2-1 with Reporting Multiple Genes Encoding the Membrane-Associated Flavoprotein-Cytochrome c Complexed Dehydrogenases |
| title_fullStr | 2,5-Diketo-D-Gluconate Hyperproducing <i>Gluconobacter sphaericus</i> SJF2-1 with Reporting Multiple Genes Encoding the Membrane-Associated Flavoprotein-Cytochrome c Complexed Dehydrogenases |
| title_full_unstemmed | 2,5-Diketo-D-Gluconate Hyperproducing <i>Gluconobacter sphaericus</i> SJF2-1 with Reporting Multiple Genes Encoding the Membrane-Associated Flavoprotein-Cytochrome c Complexed Dehydrogenases |
| title_short | 2,5-Diketo-D-Gluconate Hyperproducing <i>Gluconobacter sphaericus</i> SJF2-1 with Reporting Multiple Genes Encoding the Membrane-Associated Flavoprotein-Cytochrome c Complexed Dehydrogenases |
| title_sort | 2 5 diketo d gluconate hyperproducing i gluconobacter sphaericus i sjf2 1 with reporting multiple genes encoding the membrane associated flavoprotein cytochrome c complexed dehydrogenases |
| topic | <i>Gluconobacter sphaericus</i> 2,5-diketo-D-gluconate membrane-associated flavoprotein-cytochrome c complexed dehydrogenase L-ascorbic acid D-glucose biotransformation D-gluconate |
| url | https://www.mdpi.com/2076-2607/10/11/2130 |
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