Colicins and T6SS-based competition systems enhance enterotoxigenic E. coli (ETEC) competitiveness
ABSTRACTDiarrheal diseases are still a significant problem for humankind, causing approximately half a million deaths annually. To cause diarrhea, enteric bacterial pathogens must first colonize the gut, which is a niche occupied by the normal bacterial microbiota. Therefore, the ability of pathogen...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2024-12-01
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| Series: | Gut Microbes |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/19490976.2023.2295891 |
| _version_ | 1797374827339710464 |
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| author | Jonas Kjellin Danna Lee Hans Steinsland Rachel Dwane Oda Barth Vedoy Kurt Hanevik Sanna Koskiniemi |
| author_facet | Jonas Kjellin Danna Lee Hans Steinsland Rachel Dwane Oda Barth Vedoy Kurt Hanevik Sanna Koskiniemi |
| author_sort | Jonas Kjellin |
| collection | DOAJ |
| description | ABSTRACTDiarrheal diseases are still a significant problem for humankind, causing approximately half a million deaths annually. To cause diarrhea, enteric bacterial pathogens must first colonize the gut, which is a niche occupied by the normal bacterial microbiota. Therefore, the ability of pathogenic bacteria to inhibit the growth of other bacteria can facilitate the colonization process. Although enterotoxigenic Escherichia coli (ETEC) is one of the major causative agents of diarrheal diseases, little is known about the competition systems found in and used by ETEC and how they contribute to the ability of ETEC to colonize a host. Here, we collected a set of 94 fully assembled ETEC genomes by performing whole-genome sequencing and mining the NCBI RefSeq database. Using this set, we performed a comprehensive search for delivered bacterial toxins and investigated how these toxins contribute to ETEC competitiveness in vitro. We found that type VI secretion systems (T6SS) were widespread among ETEC (n = 47). In addition, several closely related ETEC strains were found to encode Colicin Ia and T6SS (n = 8). These toxins provide ETEC competitive advantages during in vitro competition against other E. coli, suggesting that the role of T6SS as well as colicins in ETEC biology has until now been underappreciated. |
| first_indexed | 2024-03-08T19:11:21Z |
| format | Article |
| id | doaj.art-2fd5f4aeb735487e9b2c86d088909b85 |
| institution | Directory Open Access Journal |
| issn | 1949-0976 1949-0984 |
| language | English |
| last_indexed | 2024-03-08T19:11:21Z |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Gut Microbes |
| spelling | doaj.art-2fd5f4aeb735487e9b2c86d088909b852023-12-27T11:11:03ZengTaylor & Francis GroupGut Microbes1949-09761949-09842024-12-0116110.1080/19490976.2023.2295891Colicins and T6SS-based competition systems enhance enterotoxigenic E. coli (ETEC) competitivenessJonas Kjellin0Danna Lee1Hans Steinsland2Rachel Dwane3Oda Barth Vedoy4Kurt Hanevik5Sanna Koskiniemi6Department of Cell and Molecular Biology, Uppsala University, Uppsala, SwedenDepartment of Cell and Molecular Biology, Uppsala University, Uppsala, SwedenCISMAC, Centre for International Health, Department of Global Public Health and Primary Care, University of Bergen, Bergen, NorwayDepartment of Cell and Molecular Biology, Uppsala University, Uppsala, SwedenDepartment of Clinical Science, University of Bergen, Bergen, NorwayDepartment of Clinical Science, University of Bergen, Bergen, NorwayDepartment of Cell and Molecular Biology, Uppsala University, Uppsala, SwedenABSTRACTDiarrheal diseases are still a significant problem for humankind, causing approximately half a million deaths annually. To cause diarrhea, enteric bacterial pathogens must first colonize the gut, which is a niche occupied by the normal bacterial microbiota. Therefore, the ability of pathogenic bacteria to inhibit the growth of other bacteria can facilitate the colonization process. Although enterotoxigenic Escherichia coli (ETEC) is one of the major causative agents of diarrheal diseases, little is known about the competition systems found in and used by ETEC and how they contribute to the ability of ETEC to colonize a host. Here, we collected a set of 94 fully assembled ETEC genomes by performing whole-genome sequencing and mining the NCBI RefSeq database. Using this set, we performed a comprehensive search for delivered bacterial toxins and investigated how these toxins contribute to ETEC competitiveness in vitro. We found that type VI secretion systems (T6SS) were widespread among ETEC (n = 47). In addition, several closely related ETEC strains were found to encode Colicin Ia and T6SS (n = 8). These toxins provide ETEC competitive advantages during in vitro competition against other E. coli, suggesting that the role of T6SS as well as colicins in ETEC biology has until now been underappreciated.https://www.tandfonline.com/doi/10.1080/19490976.2023.2295891ETECcolicintype VI secretioncompetitive advantagegenome |
| spellingShingle | Jonas Kjellin Danna Lee Hans Steinsland Rachel Dwane Oda Barth Vedoy Kurt Hanevik Sanna Koskiniemi Colicins and T6SS-based competition systems enhance enterotoxigenic E. coli (ETEC) competitiveness Gut Microbes ETEC colicin type VI secretion competitive advantage genome |
| title | Colicins and T6SS-based competition systems enhance enterotoxigenic E. coli (ETEC) competitiveness |
| title_full | Colicins and T6SS-based competition systems enhance enterotoxigenic E. coli (ETEC) competitiveness |
| title_fullStr | Colicins and T6SS-based competition systems enhance enterotoxigenic E. coli (ETEC) competitiveness |
| title_full_unstemmed | Colicins and T6SS-based competition systems enhance enterotoxigenic E. coli (ETEC) competitiveness |
| title_short | Colicins and T6SS-based competition systems enhance enterotoxigenic E. coli (ETEC) competitiveness |
| title_sort | colicins and t6ss based competition systems enhance enterotoxigenic e coli etec competitiveness |
| topic | ETEC colicin type VI secretion competitive advantage genome |
| url | https://www.tandfonline.com/doi/10.1080/19490976.2023.2295891 |
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