Special issue in honour of Prof. Reto J. Strasser - A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass)
Bouteloua gracilis (blue grama grass) is one of the most drought and grazing tolerant plants in the short-grass ecosystem. To obtain information on their photosystem activities, we measured the fast (< 1 s) chlorophyll a fluorescence transient (the OJIP curve) from their leaves, and isolated...
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| Format: | Article |
| Language: | English |
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Academy of Sciences of the Czech Republic, Institute of Experimental Botany
2020-05-01
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| Series: | Photosynthetica |
| Subjects: | |
| Online Access: | https://ps.ueb.cas.cz/artkey/phs-202002-0009_special-issue-in-honour-of-prof-reto-j-strasser-8211-a-comparative-chlorophyll-a-fluorescence-study-on-iso.php |
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| author | B. JIMÉNEZ-FRANCISCO A. STIRBET H. CAMPOS F.V. CONDE-MARTÍNEZ D. PADILLA-CHACÓN C. TREJO C.J. BERNACCHI G. GOVINDJEE |
| author_facet | B. JIMÉNEZ-FRANCISCO A. STIRBET H. CAMPOS F.V. CONDE-MARTÍNEZ D. PADILLA-CHACÓN C. TREJO C.J. BERNACCHI G. GOVINDJEE |
| author_sort | B. JIMÉNEZ-FRANCISCO |
| collection | DOAJ |
| description | Bouteloua gracilis (blue grama grass) is one of the most drought and grazing tolerant plants in the short-grass ecosystem. To obtain information on their photosystem activities, we measured the fast (< 1 s) chlorophyll a fluorescence transient (the OJIP curve) from their leaves, and isolated cells grown photoautotrophically in suspension in a culture medium, or with added sucrose. One of our goals was to study the effect of different sucrose concentrations (0, 0.15, 0.3, and 3%) on PSII activity in isolated cells. Our results on cells suspended in culture medium, using the JIP-test, showed a decrease in PSII activity at increasing sucrose concentrations, while the photoautotrophic cells showed an optimal PSII activity, close to that of the leaves. Further, our data on cells grown in 0, 0.15, and 0.3% sucrose, but with added CO2, and measured while the cells were deposited on filter paper, indicate that supplementary CO2 can increase the PSII activity in the presence of sucrose, although further research is necessary to understand these results. |
| first_indexed | 2024-12-11T01:00:34Z |
| format | Article |
| id | doaj.art-2fd6a7b53eb74bc581c893a198daa284 |
| institution | Directory Open Access Journal |
| issn | 0300-3604 1573-9058 |
| language | English |
| last_indexed | 2024-12-11T01:00:34Z |
| publishDate | 2020-05-01 |
| publisher | Academy of Sciences of the Czech Republic, Institute of Experimental Botany |
| record_format | Article |
| series | Photosynthetica |
| spelling | doaj.art-2fd6a7b53eb74bc581c893a198daa2842022-12-22T01:26:20ZengAcademy of Sciences of the Czech Republic, Institute of Experimental BotanyPhotosynthetica0300-36041573-90582020-05-0158226227410.32615/ps.2019.148phs-202002-0009Special issue in honour of Prof. Reto J. Strasser - A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass)B. JIMÉNEZ-FRANCISCO0A. STIRBET1H. CAMPOS2F.V. CONDE-MARTÍNEZ3D. PADILLA-CHACÓN4C. TREJO5C.J. BERNACCHI6G. GOVINDJEE7Department of Botany, Postgraduate College, Carretera México-Texcoco km 36.5, Montecillo, 56230 México, MéxicoAnne Burras Lane, Newport News, Virginia 23606, USADepartment of Botany, Postgraduate College, Carretera México-Texcoco km 36.5, Montecillo, 56230 México, MéxicoDepartment of Botany, Postgraduate College, Carretera México-Texcoco km 36.5, Montecillo, 56230 México, MéxicoDepartment of Botany, Postgraduate College, Carretera México-Texcoco km 36.5, Montecillo, 56230 México, MéxicoDepartment of Botany, Postgraduate College, Carretera México-Texcoco km 36.5, Montecillo, 56230 México, MéxicoUSDA-ARS and the Department of Plant Biology, 196 E.R. Madigan Laboratory, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USADepartment of Biochemistry, Department of Plant Biology, and Center of Biophysics & Quantitative Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USABouteloua gracilis (blue grama grass) is one of the most drought and grazing tolerant plants in the short-grass ecosystem. To obtain information on their photosystem activities, we measured the fast (< 1 s) chlorophyll a fluorescence transient (the OJIP curve) from their leaves, and isolated cells grown photoautotrophically in suspension in a culture medium, or with added sucrose. One of our goals was to study the effect of different sucrose concentrations (0, 0.15, 0.3, and 3%) on PSII activity in isolated cells. Our results on cells suspended in culture medium, using the JIP-test, showed a decrease in PSII activity at increasing sucrose concentrations, while the photoautotrophic cells showed an optimal PSII activity, close to that of the leaves. Further, our data on cells grown in 0, 0.15, and 0.3% sucrose, but with added CO2, and measured while the cells were deposited on filter paper, indicate that supplementary CO2 can increase the PSII activity in the presence of sucrose, although further research is necessary to understand these results.https://ps.ueb.cas.cz/artkey/phs-202002-0009_special-issue-in-honour-of-prof-reto-j-strasser-8211-a-comparative-chlorophyll-a-fluorescence-study-on-iso.phpkinetic parameters of the o-jj-iand i-p phases; osmotic stress; performance index |
| spellingShingle | B. JIMÉNEZ-FRANCISCO A. STIRBET H. CAMPOS F.V. CONDE-MARTÍNEZ D. PADILLA-CHACÓN C. TREJO C.J. BERNACCHI G. GOVINDJEE Special issue in honour of Prof. Reto J. Strasser - A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass) Photosynthetica kinetic parameters of the o-j j-i and i-p phases; osmotic stress; performance index |
| title | Special issue in honour of Prof. Reto J. Strasser - A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass) |
| title_full | Special issue in honour of Prof. Reto J. Strasser - A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass) |
| title_fullStr | Special issue in honour of Prof. Reto J. Strasser - A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass) |
| title_full_unstemmed | Special issue in honour of Prof. Reto J. Strasser - A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass) |
| title_short | Special issue in honour of Prof. Reto J. Strasser - A comparative chlorophyll a fluorescence study on isolated cells and intact leaves of Bouteloua gracilis (blue grama grass) |
| title_sort | special issue in honour of prof reto j strasser a comparative chlorophyll a fluorescence study on isolated cells and intact leaves of bouteloua gracilis blue grama grass |
| topic | kinetic parameters of the o-j j-i and i-p phases; osmotic stress; performance index |
| url | https://ps.ueb.cas.cz/artkey/phs-202002-0009_special-issue-in-honour-of-prof-reto-j-strasser-8211-a-comparative-chlorophyll-a-fluorescence-study-on-iso.php |
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