The microbiota of the surface, dermis and subcutaneous tissue of dog skin
Abstract Background Studies using highly sensitive molecular techniques have detected bacterial communities below the human epidermis. Depending on their abundance and composition, this finding could be clinically relevant. The aim of this study was to determine if bacteria can be detected in the de...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-09-01
|
| Series: | Animal Microbiome |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s42523-020-00050-8 |
| _version_ | 1818037528226168832 |
|---|---|
| author | Rocío García-Fonticoba Lluís Ferrer Olga Francino Anna Cuscó |
| author_facet | Rocío García-Fonticoba Lluís Ferrer Olga Francino Anna Cuscó |
| author_sort | Rocío García-Fonticoba |
| collection | DOAJ |
| description | Abstract Background Studies using highly sensitive molecular techniques have detected bacterial communities below the human epidermis. Depending on their abundance and composition, this finding could be clinically relevant. The aim of this study was to determine if bacteria can be detected in the dermis and subcutaneous tissue of dogs without cutaneous disease using two different approaches: traditional cultures and DNA sequencing of the V4 region of bacterial 16S rRNA gene using next-generation sequencing (NGS). Results Seven healthy dogs were included in the study, and two sets of samples were collected from each subject. Sample sets were composed of a 6-mm abdominal skin biopsy, including epidermis, dermis, and subcutis, a skin surface swab, and an environmental blank sample for contamination control. One set of samples from each dog was submitted for bacterial culture and the other one for bacterial DNA amplification and sequencing. Five different bacterial genera (Staphylococcus, Bacillus, Corynebacterium, Streptococcus, and Enterococcus) were isolated in five out of the seven skin surface swab samples with aerobic microbiological culture methods, while no growth was obtained from the other two samples. Although some DNA could be amplified from epidermal, dermal, and subcutaneous tissue samples, the results of the NGS were similar to those of the blanks. Conclusion When investigated with aerobic microbiological culture methods, the dermis and subcutaneous tissue of dogs are sterile. NGS techniques lead to the detection of some bacterial DNA, similar to the signal detected in blanks, which does not support the presence of a microbiota in dermis or subcutaneous tissue. |
| first_indexed | 2024-12-10T07:28:16Z |
| format | Article |
| id | doaj.art-2fd77ff2f4d9465485f457ebb9e2cd81 |
| institution | Directory Open Access Journal |
| issn | 2524-4671 |
| language | English |
| last_indexed | 2024-12-10T07:28:16Z |
| publishDate | 2020-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Animal Microbiome |
| spelling | doaj.art-2fd77ff2f4d9465485f457ebb9e2cd812022-12-22T01:57:39ZengBMCAnimal Microbiome2524-46712020-09-01211910.1186/s42523-020-00050-8The microbiota of the surface, dermis and subcutaneous tissue of dog skinRocío García-Fonticoba0Lluís Ferrer1Olga Francino2Anna Cuscó3Fundació Hospital Clínic Veterinari, Universitat Autònoma de BarcelonaFundació Hospital Clínic Veterinari, Universitat Autònoma de BarcelonaServei Veterinari de Genètica Molecular, Departament de Ciencia Animal i dels Aliments, Universitat Autònoma de BarcelonaVetgenomics, Ed. Eureka, PRUAB, Campus UABAbstract Background Studies using highly sensitive molecular techniques have detected bacterial communities below the human epidermis. Depending on their abundance and composition, this finding could be clinically relevant. The aim of this study was to determine if bacteria can be detected in the dermis and subcutaneous tissue of dogs without cutaneous disease using two different approaches: traditional cultures and DNA sequencing of the V4 region of bacterial 16S rRNA gene using next-generation sequencing (NGS). Results Seven healthy dogs were included in the study, and two sets of samples were collected from each subject. Sample sets were composed of a 6-mm abdominal skin biopsy, including epidermis, dermis, and subcutis, a skin surface swab, and an environmental blank sample for contamination control. One set of samples from each dog was submitted for bacterial culture and the other one for bacterial DNA amplification and sequencing. Five different bacterial genera (Staphylococcus, Bacillus, Corynebacterium, Streptococcus, and Enterococcus) were isolated in five out of the seven skin surface swab samples with aerobic microbiological culture methods, while no growth was obtained from the other two samples. Although some DNA could be amplified from epidermal, dermal, and subcutaneous tissue samples, the results of the NGS were similar to those of the blanks. Conclusion When investigated with aerobic microbiological culture methods, the dermis and subcutaneous tissue of dogs are sterile. NGS techniques lead to the detection of some bacterial DNA, similar to the signal detected in blanks, which does not support the presence of a microbiota in dermis or subcutaneous tissue.http://link.springer.com/article/10.1186/s42523-020-00050-8Skin microbiotaEpidermisDermisSubcutaneous tissueNext-generation sequencingDog |
| spellingShingle | Rocío García-Fonticoba Lluís Ferrer Olga Francino Anna Cuscó The microbiota of the surface, dermis and subcutaneous tissue of dog skin Animal Microbiome Skin microbiota Epidermis Dermis Subcutaneous tissue Next-generation sequencing Dog |
| title | The microbiota of the surface, dermis and subcutaneous tissue of dog skin |
| title_full | The microbiota of the surface, dermis and subcutaneous tissue of dog skin |
| title_fullStr | The microbiota of the surface, dermis and subcutaneous tissue of dog skin |
| title_full_unstemmed | The microbiota of the surface, dermis and subcutaneous tissue of dog skin |
| title_short | The microbiota of the surface, dermis and subcutaneous tissue of dog skin |
| title_sort | microbiota of the surface dermis and subcutaneous tissue of dog skin |
| topic | Skin microbiota Epidermis Dermis Subcutaneous tissue Next-generation sequencing Dog |
| url | http://link.springer.com/article/10.1186/s42523-020-00050-8 |
| work_keys_str_mv | AT rociogarciafonticoba themicrobiotaofthesurfacedermisandsubcutaneoustissueofdogskin AT lluisferrer themicrobiotaofthesurfacedermisandsubcutaneoustissueofdogskin AT olgafrancino themicrobiotaofthesurfacedermisandsubcutaneoustissueofdogskin AT annacusco themicrobiotaofthesurfacedermisandsubcutaneoustissueofdogskin AT rociogarciafonticoba microbiotaofthesurfacedermisandsubcutaneoustissueofdogskin AT lluisferrer microbiotaofthesurfacedermisandsubcutaneoustissueofdogskin AT olgafrancino microbiotaofthesurfacedermisandsubcutaneoustissueofdogskin AT annacusco microbiotaofthesurfacedermisandsubcutaneoustissueofdogskin |