Clusters of synaptic inputs on dendrites of layer 5 pyramidal cells in mouse visual cortex
The spatial organization of synaptic inputs on the dendritic tree of cortical neurons plays a major role for dendritic integration and neural computations, yet, remarkably little is known about it. We mapped the spatial organization of glutamatergic synapses between layer 5 pyramidal cells by combin...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-07-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/09222 |
| _version_ | 1811200147833487360 |
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| author | Onur Gökçe Tobias Bonhoeffer Volker Scheuss |
| author_facet | Onur Gökçe Tobias Bonhoeffer Volker Scheuss |
| author_sort | Onur Gökçe |
| collection | DOAJ |
| description | The spatial organization of synaptic inputs on the dendritic tree of cortical neurons plays a major role for dendritic integration and neural computations, yet, remarkably little is known about it. We mapped the spatial organization of glutamatergic synapses between layer 5 pyramidal cells by combining optogenetics and 2-photon calcium imaging in mouse neocortical slices. To mathematically characterize the organization of inputs we developed an approach based on combinatorial analysis of the likelihoods of specific synapse arrangements. We found that the synapses of intralaminar inputs form clusters on the basal dendrites of layer 5 pyramidal cells. These clusters contain 4 to 14 synapses within ≤30 µm of dendrite. According to the spatiotemporal characteristics of synaptic summation, these numbers suggest that there will be non-linear dendritic integration of synaptic inputs during synchronous activation. |
| first_indexed | 2024-04-12T02:00:01Z |
| format | Article |
| id | doaj.art-2fdb22474976443cbfd451fb680792c3 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:00:01Z |
| publishDate | 2016-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-2fdb22474976443cbfd451fb680792c32022-12-22T03:52:42ZengeLife Sciences Publications LtdeLife2050-084X2016-07-01510.7554/eLife.09222Clusters of synaptic inputs on dendrites of layer 5 pyramidal cells in mouse visual cortexOnur Gökçe0https://orcid.org/0000-0002-3913-3893Tobias Bonhoeffer1https://orcid.org/0000-0001-7897-6634Volker Scheuss2https://orcid.org/0000-0002-0659-3228Department Synapses - Circuits - Plasticity, Max Planck Institute of Neurobiology, Martinsried, GermanyDepartment Synapses - Circuits - Plasticity, Max Planck Institute of Neurobiology, Martinsried, GermanyDepartment Synapses - Circuits - Plasticity, Max Planck Institute of Neurobiology, Martinsried, GermanyThe spatial organization of synaptic inputs on the dendritic tree of cortical neurons plays a major role for dendritic integration and neural computations, yet, remarkably little is known about it. We mapped the spatial organization of glutamatergic synapses between layer 5 pyramidal cells by combining optogenetics and 2-photon calcium imaging in mouse neocortical slices. To mathematically characterize the organization of inputs we developed an approach based on combinatorial analysis of the likelihoods of specific synapse arrangements. We found that the synapses of intralaminar inputs form clusters on the basal dendrites of layer 5 pyramidal cells. These clusters contain 4 to 14 synapses within ≤30 µm of dendrite. According to the spatiotemporal characteristics of synaptic summation, these numbers suggest that there will be non-linear dendritic integration of synaptic inputs during synchronous activation.https://elifesciences.org/articles/09222synapse mappingsynapse clusteroptogenetics2-photon calcium imagingvisual cortexspatial cluster analysis |
| spellingShingle | Onur Gökçe Tobias Bonhoeffer Volker Scheuss Clusters of synaptic inputs on dendrites of layer 5 pyramidal cells in mouse visual cortex eLife synapse mapping synapse cluster optogenetics 2-photon calcium imaging visual cortex spatial cluster analysis |
| title | Clusters of synaptic inputs on dendrites of layer 5 pyramidal cells in mouse visual cortex |
| title_full | Clusters of synaptic inputs on dendrites of layer 5 pyramidal cells in mouse visual cortex |
| title_fullStr | Clusters of synaptic inputs on dendrites of layer 5 pyramidal cells in mouse visual cortex |
| title_full_unstemmed | Clusters of synaptic inputs on dendrites of layer 5 pyramidal cells in mouse visual cortex |
| title_short | Clusters of synaptic inputs on dendrites of layer 5 pyramidal cells in mouse visual cortex |
| title_sort | clusters of synaptic inputs on dendrites of layer 5 pyramidal cells in mouse visual cortex |
| topic | synapse mapping synapse cluster optogenetics 2-photon calcium imaging visual cortex spatial cluster analysis |
| url | https://elifesciences.org/articles/09222 |
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