Manganese effects on in vitro development of lesser centaury [Centaurium pulchellum (Sw.) Druce]

To determine the manganese requirement necessary for optimal development of lesser centaury [Centaurium pulchellum (Sw.) Druce] in vitro, we investigated the effect of exogenously applied Mn on different developmental processes such as growth, flowering, fruiting, and seed germination. The applicati...

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Main Authors: Todorović Slađana, Giba Z., Simonović Ana, Božić Dragana, Banjanac Tijana, Grubišić D.
Format: Article
Language:English
Published: University of Belgrade, University of Novi Sad 2009-01-01
Series:Archives of Biological Sciences
Subjects:
Online Access:http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640902279T.pdf
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author Todorović Slađana
Giba Z.
Simonović Ana
Božić Dragana
Banjanac Tijana
Grubišić D.
author_facet Todorović Slađana
Giba Z.
Simonović Ana
Božić Dragana
Banjanac Tijana
Grubišić D.
author_sort Todorović Slađana
collection DOAJ
description To determine the manganese requirement necessary for optimal development of lesser centaury [Centaurium pulchellum (Sw.) Druce] in vitro, we investigated the effect of exogenously applied Mn on different developmental processes such as growth, flowering, fruiting, and seed germination. The application of Mn had no effect on stem length, except at the highest concentration of 10-2 M, which was inhibitory. In addition, C. pulchellum plants were capable of in vitro flowering and fruiting even on media without added Mn. However, Mn content in the media affected seed dimensions, since both length and width of the seeds increased with increasing Mn concentration. Moreover, both excess and absence of Mn in the media caused appearance of necrotic plants. Exogenously applied Mn had no effect on seed germination percentage, except at concentrations greater than 3x10-3 M.
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spelling doaj.art-2fdc84cdf6d441dda851a0a49e0c72972022-12-22T00:27:15ZengUniversity of Belgrade, University of Novi SadArchives of Biological Sciences0354-46642009-01-0161227928310.2298/ABS0902279TManganese effects on in vitro development of lesser centaury [Centaurium pulchellum (Sw.) Druce]Todorović SlađanaGiba Z.Simonović AnaBožić DraganaBanjanac TijanaGrubišić D.To determine the manganese requirement necessary for optimal development of lesser centaury [Centaurium pulchellum (Sw.) Druce] in vitro, we investigated the effect of exogenously applied Mn on different developmental processes such as growth, flowering, fruiting, and seed germination. The application of Mn had no effect on stem length, except at the highest concentration of 10-2 M, which was inhibitory. In addition, C. pulchellum plants were capable of in vitro flowering and fruiting even on media without added Mn. However, Mn content in the media affected seed dimensions, since both length and width of the seeds increased with increasing Mn concentration. Moreover, both excess and absence of Mn in the media caused appearance of necrotic plants. Exogenously applied Mn had no effect on seed germination percentage, except at concentrations greater than 3x10-3 M.http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640902279T.pdfCentaurium pulchellummanganesefloweringfruitinggerminationin vitro
spellingShingle Todorović Slađana
Giba Z.
Simonović Ana
Božić Dragana
Banjanac Tijana
Grubišić D.
Manganese effects on in vitro development of lesser centaury [Centaurium pulchellum (Sw.) Druce]
Archives of Biological Sciences
Centaurium pulchellum
manganese
flowering
fruiting
germination
in vitro
title Manganese effects on in vitro development of lesser centaury [Centaurium pulchellum (Sw.) Druce]
title_full Manganese effects on in vitro development of lesser centaury [Centaurium pulchellum (Sw.) Druce]
title_fullStr Manganese effects on in vitro development of lesser centaury [Centaurium pulchellum (Sw.) Druce]
title_full_unstemmed Manganese effects on in vitro development of lesser centaury [Centaurium pulchellum (Sw.) Druce]
title_short Manganese effects on in vitro development of lesser centaury [Centaurium pulchellum (Sw.) Druce]
title_sort manganese effects on in vitro development of lesser centaury centaurium pulchellum sw druce
topic Centaurium pulchellum
manganese
flowering
fruiting
germination
in vitro
url http://www.doiserbia.nb.rs/img/doi/0354-4664/2009/0354-46640902279T.pdf
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AT simonovicana manganeseeffectsoninvitrodevelopmentoflessercentaurycentauriumpulchellumswdruce
AT bozicdragana manganeseeffectsoninvitrodevelopmentoflessercentaurycentauriumpulchellumswdruce
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