Transcriptomic analysis of human endometrial stromal cells during early embryo invasion
AbstractPurpose During early embryo invasion (48 h after embryo attachment), what functional changes accompany dynamic gene expression alterations in human endometrial stromal cells?Method In the present study, primary human endometrial stromal cells (phESCs) were cultured. After in vitro decidualiz...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Annals of Medicine |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/07853890.2021.1988139 |
| _version_ | 1797385683975798784 |
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| author | Shuo Han Minghui Liu Shan Liu Yuan Li |
| author_facet | Shuo Han Minghui Liu Shan Liu Yuan Li |
| author_sort | Shuo Han |
| collection | DOAJ |
| description | AbstractPurpose During early embryo invasion (48 h after embryo attachment), what functional changes accompany dynamic gene expression alterations in human endometrial stromal cells?Method In the present study, primary human endometrial stromal cells (phESCs) were cultured. After in vitro decidualization, primary human endometrial stromal cells (phESCs) were cultured with blastocysts for 48 h. During this process, blastocysts attached and invaded the phESCs (embryo-invaded primary human endometrial stromal cells, ehESCs). We performed comprehensive transcriptomic profiling of phESCs (two replicates) and ehESCs (five replicates) and analyzed the differentially expressed gene (DEGs) sets for gene ontology (GO) terms and Kyoto encyclopaedia of genes and genomes (KEGG) pathway enrichment. To analyse potential connectivity patterns between the transcripts in these DEG sets, a protein-protein interaction (PPI) network was constructed using the STRING database.Results A total of 592 DEGs were identified between phESCs and ehESCs after embryo invasion. Primary human endometrial stromal cells underwent significant transcriptomic changes that occur in a stepwise fashion. Oxidative phosphorylation, mitochondrial organization, and P53 signalling pathways were significantly altered in phESCs after embryo invasion. EP300 may play a key role in regulating transcription via chromatin remodelling to facilitate the adaptive gene expression changes that occur during embryo invasion.Conclusions Our data identify dynamic transcriptome changes that occur in endometrial stromal cells within 48 h after embryo invasion. The pathways that we found to be enriched in phESCs after embryo invasion (oxidative phosphorylation, mitochondrial organization, and P53 signalling) may represent novel mechanisms underlying embryo implantation, and may illuminate the reasons that some women experience reproductive failure.Key messagesHuman endometrial stromal cells have undergone changes in gene expression regulation and signalling pathways during the embryo invasion.Mitochondrial-oxidative phosphorylation changes in human stromal cells manifested as down-regulation of gene expression in the electron transport chain.TP53 signalling pathway and transcriptional regulator EP300 assist stromal cells to get adaptive changes during embryo invasion phase. |
| first_indexed | 2024-03-08T21:57:50Z |
| format | Article |
| id | doaj.art-2fdfe614b3b448a49b340a22e4cd49e7 |
| institution | Directory Open Access Journal |
| issn | 0785-3890 1365-2060 |
| language | English |
| last_indexed | 2024-03-08T21:57:50Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Annals of Medicine |
| spelling | doaj.art-2fdfe614b3b448a49b340a22e4cd49e72023-12-19T16:46:26ZengTaylor & Francis GroupAnnals of Medicine0785-38901365-20602021-01-015311758177110.1080/07853890.2021.1988139Transcriptomic analysis of human endometrial stromal cells during early embryo invasionShuo Han0Minghui Liu1Shan Liu2Yuan Li3Medical Center for Human Reproduction, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, ChinaMedical Center for Human Reproduction, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, ChinaMedical Center for Human Reproduction, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, ChinaMedical Center for Human Reproduction, Beijing Chao-Yang Hospital, Capital Medical University, Beijing, ChinaAbstractPurpose During early embryo invasion (48 h after embryo attachment), what functional changes accompany dynamic gene expression alterations in human endometrial stromal cells?Method In the present study, primary human endometrial stromal cells (phESCs) were cultured. After in vitro decidualization, primary human endometrial stromal cells (phESCs) were cultured with blastocysts for 48 h. During this process, blastocysts attached and invaded the phESCs (embryo-invaded primary human endometrial stromal cells, ehESCs). We performed comprehensive transcriptomic profiling of phESCs (two replicates) and ehESCs (five replicates) and analyzed the differentially expressed gene (DEGs) sets for gene ontology (GO) terms and Kyoto encyclopaedia of genes and genomes (KEGG) pathway enrichment. To analyse potential connectivity patterns between the transcripts in these DEG sets, a protein-protein interaction (PPI) network was constructed using the STRING database.Results A total of 592 DEGs were identified between phESCs and ehESCs after embryo invasion. Primary human endometrial stromal cells underwent significant transcriptomic changes that occur in a stepwise fashion. Oxidative phosphorylation, mitochondrial organization, and P53 signalling pathways were significantly altered in phESCs after embryo invasion. EP300 may play a key role in regulating transcription via chromatin remodelling to facilitate the adaptive gene expression changes that occur during embryo invasion.Conclusions Our data identify dynamic transcriptome changes that occur in endometrial stromal cells within 48 h after embryo invasion. The pathways that we found to be enriched in phESCs after embryo invasion (oxidative phosphorylation, mitochondrial organization, and P53 signalling) may represent novel mechanisms underlying embryo implantation, and may illuminate the reasons that some women experience reproductive failure.Key messagesHuman endometrial stromal cells have undergone changes in gene expression regulation and signalling pathways during the embryo invasion.Mitochondrial-oxidative phosphorylation changes in human stromal cells manifested as down-regulation of gene expression in the electron transport chain.TP53 signalling pathway and transcriptional regulator EP300 assist stromal cells to get adaptive changes during embryo invasion phase.https://www.tandfonline.com/doi/10.1080/07853890.2021.1988139Endometrial stromal cellsembryo invasiontranscriptome |
| spellingShingle | Shuo Han Minghui Liu Shan Liu Yuan Li Transcriptomic analysis of human endometrial stromal cells during early embryo invasion Annals of Medicine Endometrial stromal cells embryo invasion transcriptome |
| title | Transcriptomic analysis of human endometrial stromal cells during early embryo invasion |
| title_full | Transcriptomic analysis of human endometrial stromal cells during early embryo invasion |
| title_fullStr | Transcriptomic analysis of human endometrial stromal cells during early embryo invasion |
| title_full_unstemmed | Transcriptomic analysis of human endometrial stromal cells during early embryo invasion |
| title_short | Transcriptomic analysis of human endometrial stromal cells during early embryo invasion |
| title_sort | transcriptomic analysis of human endometrial stromal cells during early embryo invasion |
| topic | Endometrial stromal cells embryo invasion transcriptome |
| url | https://www.tandfonline.com/doi/10.1080/07853890.2021.1988139 |
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