Transgenic strategies to confer resistance against viruses in rice plants
Rice (Oryza sativa L.) is cultivated in more than 100 countries and supports nearly half of the world’s population. Developing efficient methods to control rice viruses is thus an urgent necessity because viruses cause serious losses in rice yield. Most rice viruses are transmitted by insect vectors...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2014-01-01
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| Series: | Frontiers in Microbiology |
| Subjects: | |
| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00409/full |
| _version_ | 1828422517731098624 |
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| author | Takahide eSasaya Eiko eNakazono-Nagaoka Hiroaki eSaika Hideyuki eAoki Akihiro eHiraguri Osamu eNetsu Tamaki Uehara Ichiki Masatoshi eOnuki Seiichi eToki Koji eSaito Osamu eYatou |
| author_facet | Takahide eSasaya Eiko eNakazono-Nagaoka Hiroaki eSaika Hideyuki eAoki Akihiro eHiraguri Osamu eNetsu Tamaki Uehara Ichiki Masatoshi eOnuki Seiichi eToki Koji eSaito Osamu eYatou |
| author_sort | Takahide eSasaya |
| collection | DOAJ |
| description | Rice (Oryza sativa L.) is cultivated in more than 100 countries and supports nearly half of the world’s population. Developing efficient methods to control rice viruses is thus an urgent necessity because viruses cause serious losses in rice yield. Most rice viruses are transmitted by insect vectors, notably planthoppers and leafhoppers. Viruliferous insect vectors can disperse their viruses over relatively long distances, and eradication of the viruses is very difficult once they become widespread. Exploitation of natural genetic sources of resistance is one of the most effective approaches to protect crops from virus infection; however, only a few naturally occurring rice genes confer resistance against rice viruses. In an effort to improve control, many investigators are using genetic engineering of rice plants as a potential strategy to control viral diseases. Using viral genes to confer pathogen-derived resistance against crops is a well-established procedure, and the expression of various viral gene products has proved to be effective in preventing or reducing infection by various plant viruses since the 1990s. RNA-interference (RNAi), also known as RNA silencing, is one of the most efficient methods to confer resistance against plant viruses on their respective crops. In this article, we review the recent progress, mainly conducted by our research group, in transgenic strategies to confer resistance against tenuiviruses and reoviruses in rice plants. Our findings also illustrate that not all RNAi constructs against viral RNAs are equally effective in preventing virus infection and that it is important to identify the viral Achilles’ heel gene to target for RNAi attack when engineering plants. |
| first_indexed | 2024-12-10T15:49:19Z |
| format | Article |
| id | doaj.art-2fe212d32871432eafa5a8b0c04fda71 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T15:49:19Z |
| publishDate | 2014-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-2fe212d32871432eafa5a8b0c04fda712022-12-22T01:42:51ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2014-01-01410.3389/fmicb.2013.0040964958Transgenic strategies to confer resistance against viruses in rice plantsTakahide eSasaya0Eiko eNakazono-Nagaoka1Hiroaki eSaika2Hideyuki eAoki3Akihiro eHiraguri4Osamu eNetsu5Tamaki Uehara Ichiki6Masatoshi eOnuki7Seiichi eToki8Koji eSaito9Osamu eYatou10Kyushu Okinawa Agricultural Research CenterNational Institute of Fruit Tree ScienceNational Institute of Agrobiological SciencesHokuriku Research Center, NARO Agricultural Research CenterThe University of TokyoThe University of TokyoNational Institute of Agrobiological SciencesKyushu Okinawa Agricultural Research CenterNational Institute of Agrobiological SciencesHokuriku Research Center, NARO Agricultural Research CenterHokuriku Research Center, NARO Agricultural Research CenterRice (Oryza sativa L.) is cultivated in more than 100 countries and supports nearly half of the world’s population. Developing efficient methods to control rice viruses is thus an urgent necessity because viruses cause serious losses in rice yield. Most rice viruses are transmitted by insect vectors, notably planthoppers and leafhoppers. Viruliferous insect vectors can disperse their viruses over relatively long distances, and eradication of the viruses is very difficult once they become widespread. Exploitation of natural genetic sources of resistance is one of the most effective approaches to protect crops from virus infection; however, only a few naturally occurring rice genes confer resistance against rice viruses. In an effort to improve control, many investigators are using genetic engineering of rice plants as a potential strategy to control viral diseases. Using viral genes to confer pathogen-derived resistance against crops is a well-established procedure, and the expression of various viral gene products has proved to be effective in preventing or reducing infection by various plant viruses since the 1990s. RNA-interference (RNAi), also known as RNA silencing, is one of the most efficient methods to confer resistance against plant viruses on their respective crops. In this article, we review the recent progress, mainly conducted by our research group, in transgenic strategies to confer resistance against tenuiviruses and reoviruses in rice plants. Our findings also illustrate that not all RNAi constructs against viral RNAs are equally effective in preventing virus infection and that it is important to identify the viral Achilles’ heel gene to target for RNAi attack when engineering plants.http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00409/fullReoviridaeRNA InterferenceTenuivirustransgenic riceforage rice cultivar |
| spellingShingle | Takahide eSasaya Eiko eNakazono-Nagaoka Hiroaki eSaika Hideyuki eAoki Akihiro eHiraguri Osamu eNetsu Tamaki Uehara Ichiki Masatoshi eOnuki Seiichi eToki Koji eSaito Osamu eYatou Transgenic strategies to confer resistance against viruses in rice plants Frontiers in Microbiology Reoviridae RNA Interference Tenuivirus transgenic rice forage rice cultivar |
| title | Transgenic strategies to confer resistance against viruses in rice plants |
| title_full | Transgenic strategies to confer resistance against viruses in rice plants |
| title_fullStr | Transgenic strategies to confer resistance against viruses in rice plants |
| title_full_unstemmed | Transgenic strategies to confer resistance against viruses in rice plants |
| title_short | Transgenic strategies to confer resistance against viruses in rice plants |
| title_sort | transgenic strategies to confer resistance against viruses in rice plants |
| topic | Reoviridae RNA Interference Tenuivirus transgenic rice forage rice cultivar |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2013.00409/full |
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