Characterization of the Bacterial Biofilm Communities Present in Reverse-Osmosis Water Systems for Haemodialysis
Biofilm in reverse osmosis (RO) membranes is a common problem in water treatment at haemodialysis facilities. Bacteria adhere and proliferate on RO membranes, forming biofilms, obstructing and damaging the membranes and allowing the transfer of bacteria and/or cellular components potentially harmful...
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MDPI AG
2020-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/9/1418 |
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| author | Juan-Pablo Cuevas Ruben Moraga Kimberly Sánchez-Alonzo Cristian Valenzuela Paulina Aguayo Carlos T. Smith Apolinaria García Ítalo Fernandez Víctor L Campos |
| author_facet | Juan-Pablo Cuevas Ruben Moraga Kimberly Sánchez-Alonzo Cristian Valenzuela Paulina Aguayo Carlos T. Smith Apolinaria García Ítalo Fernandez Víctor L Campos |
| author_sort | Juan-Pablo Cuevas |
| collection | DOAJ |
| description | Biofilm in reverse osmosis (RO) membranes is a common problem in water treatment at haemodialysis facilities. Bacteria adhere and proliferate on RO membranes, forming biofilms, obstructing and damaging the membranes and allowing the transfer of bacteria and/or cellular components potentially harmful to the health of haemodialysis patients. Our aim was to characterize the bacterial community associated to biofilm of RO membranes and to identify potentially pathogenic bacteria present in the haemodialysis systems of two dialysis centres in Chile. The diversity of the bacterial communities present on RO membranes and potable and osmosed water samples was evaluated using Illumina sequencing. Additionally, bacteria from potable water, osmosed water and RO membrane samples were isolated, characterized and identified by Sanger’s sequencing. The molecular analyses of metagenomics showed that the <i>phyla</i> having a greater relative abundance in both dialysis centres were <i>Proteobacteria</i> and <i>Planctomycetes. Pseudomonas, Stenotrophomonas</i>, <i>Agrobacterium</i>, <i>Pigmentiphaga</i>, <i>Ralstonia</i>, <i>Arthrobacter</i>, <i>Bacteroides</i> and <i>Staphylococcus</i> were bacterial genera isolated from the different samples obtained at both haemodialysis centres. <i>Pseudomonas</i> spp. was a bacterial genus with greater frequency in all samples. <i>Pseudomonas</i> and <i>Staphylococcus</i> showed higher levels of resistance to the antibiotics tested. Results demonstrated the presence of potentially pathogenic bacteria, showing resistance to antimicrobials on RO membranes and in osmosed water in both dialysis centres studied. |
| first_indexed | 2024-03-10T16:19:21Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T16:19:21Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-2fe6f7ec7d1e4cf1828be6265df5090c2023-11-20T13:47:13ZengMDPI AGMicroorganisms2076-26072020-09-0189141810.3390/microorganisms8091418Characterization of the Bacterial Biofilm Communities Present in Reverse-Osmosis Water Systems for HaemodialysisJuan-Pablo Cuevas0Ruben Moraga1Kimberly Sánchez-Alonzo2Cristian Valenzuela3Paulina Aguayo4Carlos T. Smith5Apolinaria García6Ítalo Fernandez7Víctor L Campos8Environmental Microbiology Laboratory, Department of Microbiology, Faculty of Biological Sciences, University of Concepción, Concepción 4070386, ChileMicrobiology Laboratory, Faculty of Renewable Natural Resources, Arturo Prat University, Iquique 1100000, ChileLaboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepcion, Concepcion 4070386, ChileEnvironmental Microbiology Laboratory, Department of Microbiology, Faculty of Biological Sciences, University of Concepción, Concepción 4070386, ChileFaculty of Environmental Sciences, EULA-Chile, Universidad de Concepcion, Concepcion 4070386, ChileLaboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepcion, Concepcion 4070386, ChileLaboratory of Bacterial Pathogenicity, Department of Microbiology, Faculty of Biological Sciences, Universidad de Concepcion, Concepcion 4070386, ChileEnvironmental Microbiology Laboratory, Department of Microbiology, Faculty of Biological Sciences, University of Concepción, Concepción 4070386, ChileEnvironmental Microbiology Laboratory, Department of Microbiology, Faculty of Biological Sciences, University of Concepción, Concepción 4070386, ChileBiofilm in reverse osmosis (RO) membranes is a common problem in water treatment at haemodialysis facilities. Bacteria adhere and proliferate on RO membranes, forming biofilms, obstructing and damaging the membranes and allowing the transfer of bacteria and/or cellular components potentially harmful to the health of haemodialysis patients. Our aim was to characterize the bacterial community associated to biofilm of RO membranes and to identify potentially pathogenic bacteria present in the haemodialysis systems of two dialysis centres in Chile. The diversity of the bacterial communities present on RO membranes and potable and osmosed water samples was evaluated using Illumina sequencing. Additionally, bacteria from potable water, osmosed water and RO membrane samples were isolated, characterized and identified by Sanger’s sequencing. The molecular analyses of metagenomics showed that the <i>phyla</i> having a greater relative abundance in both dialysis centres were <i>Proteobacteria</i> and <i>Planctomycetes. Pseudomonas, Stenotrophomonas</i>, <i>Agrobacterium</i>, <i>Pigmentiphaga</i>, <i>Ralstonia</i>, <i>Arthrobacter</i>, <i>Bacteroides</i> and <i>Staphylococcus</i> were bacterial genera isolated from the different samples obtained at both haemodialysis centres. <i>Pseudomonas</i> spp. was a bacterial genus with greater frequency in all samples. <i>Pseudomonas</i> and <i>Staphylococcus</i> showed higher levels of resistance to the antibiotics tested. Results demonstrated the presence of potentially pathogenic bacteria, showing resistance to antimicrobials on RO membranes and in osmosed water in both dialysis centres studied.https://www.mdpi.com/2076-2607/8/9/1418biofilm/biofoulingreverse osmosis membraneshaemodialysismetagenomics |
| spellingShingle | Juan-Pablo Cuevas Ruben Moraga Kimberly Sánchez-Alonzo Cristian Valenzuela Paulina Aguayo Carlos T. Smith Apolinaria García Ítalo Fernandez Víctor L Campos Characterization of the Bacterial Biofilm Communities Present in Reverse-Osmosis Water Systems for Haemodialysis Microorganisms biofilm/biofouling reverse osmosis membranes haemodialysis metagenomics |
| title | Characterization of the Bacterial Biofilm Communities Present in Reverse-Osmosis Water Systems for Haemodialysis |
| title_full | Characterization of the Bacterial Biofilm Communities Present in Reverse-Osmosis Water Systems for Haemodialysis |
| title_fullStr | Characterization of the Bacterial Biofilm Communities Present in Reverse-Osmosis Water Systems for Haemodialysis |
| title_full_unstemmed | Characterization of the Bacterial Biofilm Communities Present in Reverse-Osmosis Water Systems for Haemodialysis |
| title_short | Characterization of the Bacterial Biofilm Communities Present in Reverse-Osmosis Water Systems for Haemodialysis |
| title_sort | characterization of the bacterial biofilm communities present in reverse osmosis water systems for haemodialysis |
| topic | biofilm/biofouling reverse osmosis membranes haemodialysis metagenomics |
| url | https://www.mdpi.com/2076-2607/8/9/1418 |
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