Function of Cajal Bodies in Nuclear RNA Retention in <i>A. thaliana</i> Leaves Subjected to Hypoxia
Retention of RNA in the nucleus precisely regulates the time and rate of translation and controls transcriptional bursts that can generate profound variability in mRNA levels among identical cells in tissues. In this study, we investigated the function of Cajal bodies (CBs) in RNA retention in <i...
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| Format: | Article |
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MDPI AG
2022-07-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/14/7568 |
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| author | Sylwia Górka Dawid Kubiak Małgorzata Ciesińska Katarzyna Niedojadło Jarosław Tyburski Janusz Niedojadło |
| author_facet | Sylwia Górka Dawid Kubiak Małgorzata Ciesińska Katarzyna Niedojadło Jarosław Tyburski Janusz Niedojadło |
| author_sort | Sylwia Górka |
| collection | DOAJ |
| description | Retention of RNA in the nucleus precisely regulates the time and rate of translation and controls transcriptional bursts that can generate profound variability in mRNA levels among identical cells in tissues. In this study, we investigated the function of Cajal bodies (CBs) in RNA retention in <i>A. thaliana</i> leaf nuclei during hypoxia stress was investigated. It was observed that in <i>ncb-1</i> mutants with a complete absence of CBs, the accumulation of poly(A<sup>+</sup>) RNA in the leaf nuclei was lower than that in wt under stress. Moreover, unlike in root cells, CBs store less RNA, and RNA retention in the nuclei is much less intense. Our results reveal that the function of CBs in the accumulation of RNA in nuclei under stress depends on the plant organ. Additionally, in <i>ncb-1</i>, retention of introns of mRNA RPB1 (largest subunit of RNA polymerase II) mRNA was observed. However, this isoform is highly accumulated in the nucleus. It thus follows that intron retention in transcripts is more important than CBs for the accumulation of RNA in nuclei. Accumulated mRNAs with introns in the nucleus could escape transcript degradation by NMD (nonsense-mediated mRNA decay). From non-fully spliced mRNAs in <i>ncb-1</i> nuclei, whose levels increase during hypoxia, introns are removed during reoxygenation. Then, the mRNA is transferred to the cytoplasm, and the RPB1 protein is translated. Despite the accumulation of isoforms in nuclei with retention of introns in reoxygenation, <i>ncb-1</i> coped much worse with long hypoxia, and manifested faster yellowing and shrinkage of leaves. |
| first_indexed | 2024-03-09T10:18:06Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T10:18:06Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-2fea331ca7c7437c82a0cb39a1fcf5952023-12-01T22:14:02ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-07-012314756810.3390/ijms23147568Function of Cajal Bodies in Nuclear RNA Retention in <i>A. thaliana</i> Leaves Subjected to HypoxiaSylwia Górka0Dawid Kubiak1Małgorzata Ciesińska2Katarzyna Niedojadło3Jarosław Tyburski4Janusz Niedojadło5Department of Cellular and Molecular Biology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, PolandDepartment of Cellular and Molecular Biology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, PolandDepartment of Cellular and Molecular Biology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, PolandDepartment of Cellular and Molecular Biology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, PolandChair of Plant Physiology and Biotechnology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, PolandDepartment of Cellular and Molecular Biology, Nicolaus Copernicus University, Lwowska 1, 87-100 Toruń, PolandRetention of RNA in the nucleus precisely regulates the time and rate of translation and controls transcriptional bursts that can generate profound variability in mRNA levels among identical cells in tissues. In this study, we investigated the function of Cajal bodies (CBs) in RNA retention in <i>A. thaliana</i> leaf nuclei during hypoxia stress was investigated. It was observed that in <i>ncb-1</i> mutants with a complete absence of CBs, the accumulation of poly(A<sup>+</sup>) RNA in the leaf nuclei was lower than that in wt under stress. Moreover, unlike in root cells, CBs store less RNA, and RNA retention in the nuclei is much less intense. Our results reveal that the function of CBs in the accumulation of RNA in nuclei under stress depends on the plant organ. Additionally, in <i>ncb-1</i>, retention of introns of mRNA RPB1 (largest subunit of RNA polymerase II) mRNA was observed. However, this isoform is highly accumulated in the nucleus. It thus follows that intron retention in transcripts is more important than CBs for the accumulation of RNA in nuclei. Accumulated mRNAs with introns in the nucleus could escape transcript degradation by NMD (nonsense-mediated mRNA decay). From non-fully spliced mRNAs in <i>ncb-1</i> nuclei, whose levels increase during hypoxia, introns are removed during reoxygenation. Then, the mRNA is transferred to the cytoplasm, and the RPB1 protein is translated. Despite the accumulation of isoforms in nuclei with retention of introns in reoxygenation, <i>ncb-1</i> coped much worse with long hypoxia, and manifested faster yellowing and shrinkage of leaves.https://www.mdpi.com/1422-0067/23/14/7568Cajal bodiesnuclear retentionintron retentionhypoxia |
| spellingShingle | Sylwia Górka Dawid Kubiak Małgorzata Ciesińska Katarzyna Niedojadło Jarosław Tyburski Janusz Niedojadło Function of Cajal Bodies in Nuclear RNA Retention in <i>A. thaliana</i> Leaves Subjected to Hypoxia International Journal of Molecular Sciences Cajal bodies nuclear retention intron retention hypoxia |
| title | Function of Cajal Bodies in Nuclear RNA Retention in <i>A. thaliana</i> Leaves Subjected to Hypoxia |
| title_full | Function of Cajal Bodies in Nuclear RNA Retention in <i>A. thaliana</i> Leaves Subjected to Hypoxia |
| title_fullStr | Function of Cajal Bodies in Nuclear RNA Retention in <i>A. thaliana</i> Leaves Subjected to Hypoxia |
| title_full_unstemmed | Function of Cajal Bodies in Nuclear RNA Retention in <i>A. thaliana</i> Leaves Subjected to Hypoxia |
| title_short | Function of Cajal Bodies in Nuclear RNA Retention in <i>A. thaliana</i> Leaves Subjected to Hypoxia |
| title_sort | function of cajal bodies in nuclear rna retention in i a thaliana i leaves subjected to hypoxia |
| topic | Cajal bodies nuclear retention intron retention hypoxia |
| url | https://www.mdpi.com/1422-0067/23/14/7568 |
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