RNA binding profile analysis of N-acetyltransferase 10 in K562 cells
Objective To delineate RNA binding profile of NAT10(N-acetyltransferase 10) in human chronic myelogenous leukemia cell line K562. Methods Enhanced UV crosslinking and immunoprecipitation (eCLIP) were used to capture the transcript collection bound by NAT10 in K562 cells. Data were obtained through...
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| Format: | Article |
| Language: | zho |
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Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.
2023-05-01
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| Series: | Jichu yixue yu linchuang |
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| Online Access: | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-5-739.pdf |
| _version_ | 1797366335458508800 |
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| author | LI Gaoyuan, WANG Yanran, WANG Fang, YU Jia |
| author_facet | LI Gaoyuan, WANG Yanran, WANG Fang, YU Jia |
| author_sort | LI Gaoyuan, WANG Yanran, WANG Fang, YU Jia |
| collection | DOAJ |
| description | Objective To delineate RNA binding profile of NAT10(N-acetyltransferase 10) in human chronic myelogenous leukemia cell line K562. Methods Enhanced UV crosslinking and immunoprecipitation (eCLIP) were used to capture the transcript collection bound by NAT10 in K562 cells. Data were obtained through high throughput sequencing and then analyzed with bioinformatics. The type and regions of NAT10 binding genes were identified via peak-calling and annotation. The function of the binding RNA was further explored by gene function enrichment analysis. Results NAT10 binding transcripts which were enriched in 3′UTR region, were mainly protein coding genes. Further analysis showed that they were functionally associated with DNA damage and repair. Conclusions NAT10 may regulate gene expression by binding to the 3′UTR region of mRNA associated with DNA damage repair. |
| first_indexed | 2024-03-08T17:02:46Z |
| format | Article |
| id | doaj.art-2fec463f930441cab0ae63674ea365ca |
| institution | Directory Open Access Journal |
| issn | 1001-6325 |
| language | zho |
| last_indexed | 2024-03-08T17:02:46Z |
| publishDate | 2023-05-01 |
| publisher | Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College. |
| record_format | Article |
| series | Jichu yixue yu linchuang |
| spelling | doaj.art-2fec463f930441cab0ae63674ea365ca2024-01-04T07:32:51ZzhoInstitute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College.Jichu yixue yu linchuang1001-63252023-05-0143573974410.16352/j.issn.1001-6325.2023.05.0739RNA binding profile analysis of N-acetyltransferase 10 in K562 cellsLI Gaoyuan, WANG Yanran, WANG Fang, YU Jia0State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences CAMS, School of Basic Medicine PUMC, Beijing 100005, ChinaObjective To delineate RNA binding profile of NAT10(N-acetyltransferase 10) in human chronic myelogenous leukemia cell line K562. Methods Enhanced UV crosslinking and immunoprecipitation (eCLIP) were used to capture the transcript collection bound by NAT10 in K562 cells. Data were obtained through high throughput sequencing and then analyzed with bioinformatics. The type and regions of NAT10 binding genes were identified via peak-calling and annotation. The function of the binding RNA was further explored by gene function enrichment analysis. Results NAT10 binding transcripts which were enriched in 3′UTR region, were mainly protein coding genes. Further analysis showed that they were functionally associated with DNA damage and repair. Conclusions NAT10 may regulate gene expression by binding to the 3′UTR region of mRNA associated with DNA damage repair.http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-5-739.pdfn-acetyltransferase 10|k562 cells|enhanced uv crosslinking, immunoprecipitation, and high-throughput sequencing(eclip-seq) |
| spellingShingle | LI Gaoyuan, WANG Yanran, WANG Fang, YU Jia RNA binding profile analysis of N-acetyltransferase 10 in K562 cells Jichu yixue yu linchuang n-acetyltransferase 10|k562 cells|enhanced uv crosslinking, immunoprecipitation, and high-throughput sequencing(eclip-seq) |
| title | RNA binding profile analysis of N-acetyltransferase 10 in K562 cells |
| title_full | RNA binding profile analysis of N-acetyltransferase 10 in K562 cells |
| title_fullStr | RNA binding profile analysis of N-acetyltransferase 10 in K562 cells |
| title_full_unstemmed | RNA binding profile analysis of N-acetyltransferase 10 in K562 cells |
| title_short | RNA binding profile analysis of N-acetyltransferase 10 in K562 cells |
| title_sort | rna binding profile analysis of n acetyltransferase 10 in k562 cells |
| topic | n-acetyltransferase 10|k562 cells|enhanced uv crosslinking, immunoprecipitation, and high-throughput sequencing(eclip-seq) |
| url | http://journal11.magtechjournal.com/Jwk_jcyxylc/fileup/1001-6325/PDF/1001-6325-2023-43-5-739.pdf |
| work_keys_str_mv | AT ligaoyuanwangyanranwangfangyujia rnabindingprofileanalysisofnacetyltransferase10ink562cells |