Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice
BackgroundSome evidence suggests that cannabidiol (CBD) has potential to help alleviate HIV symptoms due to its antioxidant and anti-inflammatory properties. Here we examined acute CBD effects on various behaviors and the endocannabinoid system in HIV Tat transgenic mice.MethodsTat transgenic mice (...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-03-01
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| Series: | Frontiers in Neuroscience |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnins.2024.1358555/full |
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| author | Barkha J. Yadav-Samudrala Benjamin L. Gorman Karenna M. Barmada Havilah P. Ravula Caitlin J. Huguely E. Diane Wallace Michelle R. Peace Justin L. Poklis Wei Jiang Wei Jiang Sylvia Fitting |
| author_facet | Barkha J. Yadav-Samudrala Benjamin L. Gorman Karenna M. Barmada Havilah P. Ravula Caitlin J. Huguely E. Diane Wallace Michelle R. Peace Justin L. Poklis Wei Jiang Wei Jiang Sylvia Fitting |
| author_sort | Barkha J. Yadav-Samudrala |
| collection | DOAJ |
| description | BackgroundSome evidence suggests that cannabidiol (CBD) has potential to help alleviate HIV symptoms due to its antioxidant and anti-inflammatory properties. Here we examined acute CBD effects on various behaviors and the endocannabinoid system in HIV Tat transgenic mice.MethodsTat transgenic mice (female/male) were injected with CBD (3, 10, 30 mg/kg) and assessed for antinociception, activity, coordination, anxiety-like behavior, and recognition memory. Brains were taken to quantify endocannabinoids, cannabinoid receptors, and cannabinoid catabolic enzymes. Additionally, CBD and metabolite 7-hydroxy-CBD were quantified in the plasma and cortex.ResultsTat decreased supraspinal-related nociception and locomotion. CBD and sex had little to no effects on any of the behavioral measures. For the endocannabinoid system male sex was associated with elevated concentration of the proinflammatory metabolite arachidonic acid in various CNS regions, including the cerebellum that also showed higher FAAH expression levels for Tat(+) males. GPR55 expression levels in the striatum and cerebellum were higher for females compared to males. CBD metabolism was altered by sex and Tat expression.ConclusionFindings indicate that acute CBD effects are not altered by HIV Tat, and acute CBD has no to minimal effects on behavior and the endocannabinoid system. |
| first_indexed | 2024-03-07T15:33:27Z |
| format | Article |
| id | doaj.art-2fec4b7aa76b45f991fe77270c26bfba |
| institution | Directory Open Access Journal |
| issn | 1662-453X |
| language | English |
| last_indexed | 2024-03-07T15:33:27Z |
| publishDate | 2024-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Neuroscience |
| spelling | doaj.art-2fec4b7aa76b45f991fe77270c26bfba2024-03-05T13:08:11ZengFrontiers Media S.A.Frontiers in Neuroscience1662-453X2024-03-011810.3389/fnins.2024.13585551358555Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male miceBarkha J. Yadav-Samudrala0Benjamin L. Gorman1Karenna M. Barmada2Havilah P. Ravula3Caitlin J. Huguely4E. Diane Wallace5Michelle R. Peace6Justin L. Poklis7Wei Jiang8Wei Jiang9Sylvia Fitting10Department of Psychology and Neuroscience, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Psychology and Neuroscience, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Psychology and Neuroscience, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Psychology and Neuroscience, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Psychology and Neuroscience, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesDepartment of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA, United StatesDepartment of Pharmacology and Toxicology, Virginia Commonwealth University, Richmond, VA, United StatesDepartment of Microbiology and Immunology, Medical University of South Carolina, Charleston, SC, United StatesDivision of Infectious Diseases, Department of Medicine, Medical University of South Carolina, Charleston, SC, United StatesDepartment of Psychology and Neuroscience, University of North Carolina at Chapel Hill, Chapel Hill, NC, United StatesBackgroundSome evidence suggests that cannabidiol (CBD) has potential to help alleviate HIV symptoms due to its antioxidant and anti-inflammatory properties. Here we examined acute CBD effects on various behaviors and the endocannabinoid system in HIV Tat transgenic mice.MethodsTat transgenic mice (female/male) were injected with CBD (3, 10, 30 mg/kg) and assessed for antinociception, activity, coordination, anxiety-like behavior, and recognition memory. Brains were taken to quantify endocannabinoids, cannabinoid receptors, and cannabinoid catabolic enzymes. Additionally, CBD and metabolite 7-hydroxy-CBD were quantified in the plasma and cortex.ResultsTat decreased supraspinal-related nociception and locomotion. CBD and sex had little to no effects on any of the behavioral measures. For the endocannabinoid system male sex was associated with elevated concentration of the proinflammatory metabolite arachidonic acid in various CNS regions, including the cerebellum that also showed higher FAAH expression levels for Tat(+) males. GPR55 expression levels in the striatum and cerebellum were higher for females compared to males. CBD metabolism was altered by sex and Tat expression.ConclusionFindings indicate that acute CBD effects are not altered by HIV Tat, and acute CBD has no to minimal effects on behavior and the endocannabinoid system.https://www.frontiersin.org/articles/10.3389/fnins.2024.1358555/fullcannabidiolTat transgenic miceantinociception2-arachidonoylglycerolarachidonic acidGPR55 |
| spellingShingle | Barkha J. Yadav-Samudrala Benjamin L. Gorman Karenna M. Barmada Havilah P. Ravula Caitlin J. Huguely E. Diane Wallace Michelle R. Peace Justin L. Poklis Wei Jiang Wei Jiang Sylvia Fitting Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice Frontiers in Neuroscience cannabidiol Tat transgenic mice antinociception 2-arachidonoylglycerol arachidonic acid GPR55 |
| title | Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice |
| title_full | Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice |
| title_fullStr | Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice |
| title_full_unstemmed | Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice |
| title_short | Effects of acute cannabidiol on behavior and the endocannabinoid system in HIV-1 Tat transgenic female and male mice |
| title_sort | effects of acute cannabidiol on behavior and the endocannabinoid system in hiv 1 tat transgenic female and male mice |
| topic | cannabidiol Tat transgenic mice antinociception 2-arachidonoylglycerol arachidonic acid GPR55 |
| url | https://www.frontiersin.org/articles/10.3389/fnins.2024.1358555/full |
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