Overexpression of ErbB-1 (EGFR) Protein in Eutopic Endometrium of Infertile Women with Severe Ovarian Endometriosis during the ‘Implantation Window’ of Menstrual Cycle
The strong association between endometriosis and infertility is of high clinical significance. High proliferative bias in eutopic endometrium during the secretory phase is a hallmark of endometriosis, which may result in high occurrence of implantation failure and resultant infertility in endometrio...
| Những tác giả chính: | , , , , , , |
|---|---|
| Định dạng: | Bài viết |
| Ngôn ngữ: | English |
| Được phát hành: |
MDPI AG
2022-10-01
|
| Loạt: | Reproductive Medicine |
| Những chủ đề: | |
| Truy cập trực tuyến: | https://www.mdpi.com/2673-3897/3/4/22 |
| _version_ | 1827637003455299584 |
|---|---|
| author | Jeevitha Poorasamy Deepali Garg Juhi Bharti Aruna Nambirajan Asmita Patil Jayasree Sengupta Debabrata Ghosh |
| author_facet | Jeevitha Poorasamy Deepali Garg Juhi Bharti Aruna Nambirajan Asmita Patil Jayasree Sengupta Debabrata Ghosh |
| author_sort | Jeevitha Poorasamy |
| collection | DOAJ |
| description | The strong association between endometriosis and infertility is of high clinical significance. High proliferative bias in eutopic endometrium during the secretory phase is a hallmark of endometriosis, which may result in high occurrence of implantation failure and resultant infertility in endometriosis. The ErbB family of proteins regulates the proliferation capacity in the endometrium, potentially causing endometrial hostility to the implantation process in endometriosis. However, our knowledge regarding the involvement of the ErbB family in human endometrium during the window of implantation (WOI) in endometriosis-associated infertility is scant. In the present study, the cellular profiles of immunopositive ErbBs-1 to -4 in the endometrium of endometriosis-free, infertile women (Group 1; <i>n</i> = 11) and in eutopic endometrium of infertile women diagnosed with stage IV ovarian endometriosis (Group 2; <i>n</i> = 13) during the mid-secretory phase were compared using standardized guidelines. Computer-aided standardized combinative analysis of immunoprecipitation in different compartments revealed an overexpression of ErbB-1 in the epithelial, stromal and vascular compartments, along with marginally higher ErbB-3 expression (<i>p</i> < 0.06) in the vascular compartment and ErbB-4 expression (<i>p</i> < 0.05) in the glandular epithelium and stroma in the endometrium during the WOI in women with primary infertility associated with stage IV ovarian endometriosis compared with disease-free endometrium of control infertile women. It appears that changes in ErbBs in the eutopic endometrium during WOI induce anomalous proliferative, inflammatory and angiogenic activities in it, which can antagonize endometrial preparation for embryo implantation in endometriosis. This knowledge appears usable in strategizing methods for the treatment of endometriosis-associated infertility, as well as preempting the oncogenic potential of endometriosis. |
| first_indexed | 2024-03-09T15:53:21Z |
| format | Article |
| id | doaj.art-2fecc091f33e4c68b30a66f687eb6c7c |
| institution | Directory Open Access Journal |
| issn | 2673-3897 |
| language | English |
| last_indexed | 2024-03-09T15:53:21Z |
| publishDate | 2022-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Reproductive Medicine |
| spelling | doaj.art-2fecc091f33e4c68b30a66f687eb6c7c2023-11-24T17:49:28ZengMDPI AGReproductive Medicine2673-38972022-10-013428029610.3390/reprodmed3040022Overexpression of ErbB-1 (EGFR) Protein in Eutopic Endometrium of Infertile Women with Severe Ovarian Endometriosis during the ‘Implantation Window’ of Menstrual CycleJeevitha Poorasamy0Deepali Garg1Juhi Bharti2Aruna Nambirajan3Asmita Patil4Jayasree Sengupta5Debabrata Ghosh6Department of Physiology, All India Institute of Medical Sciences, New Delhi 110029, IndiaDepartment of Obstetrics and Gynecology, All India Institute of Medical Sciences, New Delhi 110029, IndiaDepartment of Obstetrics and Gynecology, All India Institute of Medical Sciences, New Delhi 110029, IndiaDepartment of Pathology, All India Institute of Medical Sciences, New Delhi 110029, IndiaDepartment of Physiology, All India Institute of Medical Sciences, New Delhi 110029, IndiaDepartment of Physiology, All India Institute of Medical Sciences, New Delhi 110029, IndiaDepartment of Physiology, All India Institute of Medical Sciences, New Delhi 110029, IndiaThe strong association between endometriosis and infertility is of high clinical significance. High proliferative bias in eutopic endometrium during the secretory phase is a hallmark of endometriosis, which may result in high occurrence of implantation failure and resultant infertility in endometriosis. The ErbB family of proteins regulates the proliferation capacity in the endometrium, potentially causing endometrial hostility to the implantation process in endometriosis. However, our knowledge regarding the involvement of the ErbB family in human endometrium during the window of implantation (WOI) in endometriosis-associated infertility is scant. In the present study, the cellular profiles of immunopositive ErbBs-1 to -4 in the endometrium of endometriosis-free, infertile women (Group 1; <i>n</i> = 11) and in eutopic endometrium of infertile women diagnosed with stage IV ovarian endometriosis (Group 2; <i>n</i> = 13) during the mid-secretory phase were compared using standardized guidelines. Computer-aided standardized combinative analysis of immunoprecipitation in different compartments revealed an overexpression of ErbB-1 in the epithelial, stromal and vascular compartments, along with marginally higher ErbB-3 expression (<i>p</i> < 0.06) in the vascular compartment and ErbB-4 expression (<i>p</i> < 0.05) in the glandular epithelium and stroma in the endometrium during the WOI in women with primary infertility associated with stage IV ovarian endometriosis compared with disease-free endometrium of control infertile women. It appears that changes in ErbBs in the eutopic endometrium during WOI induce anomalous proliferative, inflammatory and angiogenic activities in it, which can antagonize endometrial preparation for embryo implantation in endometriosis. This knowledge appears usable in strategizing methods for the treatment of endometriosis-associated infertility, as well as preempting the oncogenic potential of endometriosis.https://www.mdpi.com/2673-3897/3/4/22endometriosisendometriumErbB receptorsimmunoprecipitationimplantation stageinfertility |
| spellingShingle | Jeevitha Poorasamy Deepali Garg Juhi Bharti Aruna Nambirajan Asmita Patil Jayasree Sengupta Debabrata Ghosh Overexpression of ErbB-1 (EGFR) Protein in Eutopic Endometrium of Infertile Women with Severe Ovarian Endometriosis during the ‘Implantation Window’ of Menstrual Cycle Reproductive Medicine endometriosis endometrium ErbB receptors immunoprecipitation implantation stage infertility |
| title | Overexpression of ErbB-1 (EGFR) Protein in Eutopic Endometrium of Infertile Women with Severe Ovarian Endometriosis during the ‘Implantation Window’ of Menstrual Cycle |
| title_full | Overexpression of ErbB-1 (EGFR) Protein in Eutopic Endometrium of Infertile Women with Severe Ovarian Endometriosis during the ‘Implantation Window’ of Menstrual Cycle |
| title_fullStr | Overexpression of ErbB-1 (EGFR) Protein in Eutopic Endometrium of Infertile Women with Severe Ovarian Endometriosis during the ‘Implantation Window’ of Menstrual Cycle |
| title_full_unstemmed | Overexpression of ErbB-1 (EGFR) Protein in Eutopic Endometrium of Infertile Women with Severe Ovarian Endometriosis during the ‘Implantation Window’ of Menstrual Cycle |
| title_short | Overexpression of ErbB-1 (EGFR) Protein in Eutopic Endometrium of Infertile Women with Severe Ovarian Endometriosis during the ‘Implantation Window’ of Menstrual Cycle |
| title_sort | overexpression of erbb 1 egfr protein in eutopic endometrium of infertile women with severe ovarian endometriosis during the implantation window of menstrual cycle |
| topic | endometriosis endometrium ErbB receptors immunoprecipitation implantation stage infertility |
| url | https://www.mdpi.com/2673-3897/3/4/22 |
| work_keys_str_mv | AT jeevithapoorasamy overexpressionoferbb1egfrproteinineutopicendometriumofinfertilewomenwithsevereovarianendometriosisduringtheimplantationwindowofmenstrualcycle AT deepaligarg overexpressionoferbb1egfrproteinineutopicendometriumofinfertilewomenwithsevereovarianendometriosisduringtheimplantationwindowofmenstrualcycle AT juhibharti overexpressionoferbb1egfrproteinineutopicendometriumofinfertilewomenwithsevereovarianendometriosisduringtheimplantationwindowofmenstrualcycle AT arunanambirajan overexpressionoferbb1egfrproteinineutopicendometriumofinfertilewomenwithsevereovarianendometriosisduringtheimplantationwindowofmenstrualcycle AT asmitapatil overexpressionoferbb1egfrproteinineutopicendometriumofinfertilewomenwithsevereovarianendometriosisduringtheimplantationwindowofmenstrualcycle AT jayasreesengupta overexpressionoferbb1egfrproteinineutopicendometriumofinfertilewomenwithsevereovarianendometriosisduringtheimplantationwindowofmenstrualcycle AT debabrataghosh overexpressionoferbb1egfrproteinineutopicendometriumofinfertilewomenwithsevereovarianendometriosisduringtheimplantationwindowofmenstrualcycle |