Effect of geraninn on acute lung injury induced by lipopolysaccharide in mice
Objective To explore the protective mechanism of geraninn on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice. Methods Forty-eight BALB/c mice (female, 4 weeks old, 18~22 g) were randomly divided into 6 groups (n=8): the blank group(control group), the LPS group, the dexamethasone...
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Editorial Office of Journal of Army Medical University
2022-11-01
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| Series: | 陆军军医大学学报 |
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| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202204188.htm |
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| author | WANG Jing LI Xue ZHU Qinglin WANG Wenting LI Wanrong |
| author_facet | WANG Jing LI Xue ZHU Qinglin WANG Wenting LI Wanrong |
| author_sort | WANG Jing |
| collection | DOAJ |
| description | Objective To explore the protective mechanism of geraninn on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice. Methods Forty-eight BALB/c mice (female, 4 weeks old, 18~22 g) were randomly divided into 6 groups (n=8): the blank group(control group), the LPS group, the dexamethasone group (Dex group, 2mg/kg), the geraninn low dose group (LD group, 10 mg/kg), the geraninn medium dose group (MD gruop, 20 mg/kg) and the geraninn high dose group (HD group, 40 mg/kg). The mice in blank group and LPS group were fed with normal saline, the mice in the Dex group were fed with dexamethasone and the mice in geraniin groups were given corresponding dose of drugs. Normal saline and all tested drugs were given once a day for 3 d. In 1 h later after the last administration, the acute lung injury model was established by using intranasal drip of LPS (4 mg/kg) in all groups except the blank group. In 6 h after modeling, the wet-to-dry (W/D) weight ratio of the lung tissues of mice in each group was collected. The pathological changes of lung tissue were observed by HE staining, and the number of inflammatory cells in broncho alveolar lavage fluid (BALF) and blood was measured; The levels of interleukin-6 (IL-6), interleukin-1β and tumor necrosis factor α (TNF-α) in plasma and BALF were detected by ELISA. The amount of p-NF-κBp65 was detected by immunohistochemical method. Results Compared with the blank group, the alveolar structure in LPS group was destroyed, inflammatory cell infiltrated, and the contents of IL-6, IL-1β and TNF-α in serum and BALF in the LPS group increased; The W/D weight ratio of the lung tissues, the number of macrophages and neutrophils in BALF and serum, the relative expression of p-NF-κBp65 in lung tissue also increased(P < 0.01). Compared with the LPS group, pathological state of the lung tissues improved obviously among the groups (except the blank group); The contents of IL-6, IL-1β, TNF-α in the serum and BALF, the W/D ratio of the lung tissues, the numbers of macrophages and neutrophils in BALF and the relative expression of p-NF-κBp65 of the lung tissue decreased evidently(P < 0.05). Conclusion Geraninn has a protective effect on acute lung injury in mice, and its mechanism may be related to the effect that geraninn leads to repression of p-NF-κB p65 and reduces the expression of inflammatory factors (IL-6, IL-1β and TNF-α).
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| first_indexed | 2024-04-11T15:22:19Z |
| format | Article |
| id | doaj.art-2ff04044901a4a0994ac48e48943400b |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-04-11T15:22:19Z |
| publishDate | 2022-11-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-2ff04044901a4a0994ac48e48943400b2022-12-22T04:16:20ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272022-11-0144222300230610.16016/j.2097-0927.202204188Effect of geraninn on acute lung injury induced by lipopolysaccharide in miceWANG Jing0LI Xue1ZHU Qinglin2 WANG Wenting3LI Wanrong4School of Biological and Food Engineering, Suzhou University, Suzhou, Anhui Province, 234000, ChinaSchool of Biological and Food Engineering, Suzhou University, Suzhou, Anhui Province, 234000, ChinaSchool of Biological and Food Engineering, Suzhou University, Suzhou, Anhui Province, 234000, ChinaSchool of Biological and Food Engineering, Suzhou University, Suzhou, Anhui Province, 234000, ChinaSchool of Biological and Food Engineering, Suzhou University, Suzhou, Anhui Province, 234000, ChinaObjective To explore the protective mechanism of geraninn on acute lung injury (ALI) induced by lipopolysaccharide (LPS) in mice. Methods Forty-eight BALB/c mice (female, 4 weeks old, 18~22 g) were randomly divided into 6 groups (n=8): the blank group(control group), the LPS group, the dexamethasone group (Dex group, 2mg/kg), the geraninn low dose group (LD group, 10 mg/kg), the geraninn medium dose group (MD gruop, 20 mg/kg) and the geraninn high dose group (HD group, 40 mg/kg). The mice in blank group and LPS group were fed with normal saline, the mice in the Dex group were fed with dexamethasone and the mice in geraniin groups were given corresponding dose of drugs. Normal saline and all tested drugs were given once a day for 3 d. In 1 h later after the last administration, the acute lung injury model was established by using intranasal drip of LPS (4 mg/kg) in all groups except the blank group. In 6 h after modeling, the wet-to-dry (W/D) weight ratio of the lung tissues of mice in each group was collected. The pathological changes of lung tissue were observed by HE staining, and the number of inflammatory cells in broncho alveolar lavage fluid (BALF) and blood was measured; The levels of interleukin-6 (IL-6), interleukin-1β and tumor necrosis factor α (TNF-α) in plasma and BALF were detected by ELISA. The amount of p-NF-κBp65 was detected by immunohistochemical method. Results Compared with the blank group, the alveolar structure in LPS group was destroyed, inflammatory cell infiltrated, and the contents of IL-6, IL-1β and TNF-α in serum and BALF in the LPS group increased; The W/D weight ratio of the lung tissues, the number of macrophages and neutrophils in BALF and serum, the relative expression of p-NF-κBp65 in lung tissue also increased(P < 0.01). Compared with the LPS group, pathological state of the lung tissues improved obviously among the groups (except the blank group); The contents of IL-6, IL-1β, TNF-α in the serum and BALF, the W/D ratio of the lung tissues, the numbers of macrophages and neutrophils in BALF and the relative expression of p-NF-κBp65 of the lung tissue decreased evidently(P < 0.05). Conclusion Geraninn has a protective effect on acute lung injury in mice, and its mechanism may be related to the effect that geraninn leads to repression of p-NF-κB p65 and reduces the expression of inflammatory factors (IL-6, IL-1β and TNF-α). http://aammt.tmmu.edu.cn/Upload/rhtml/202204188.htmgeraninnacute lung injurylpsp-nf-κbp65 |
| spellingShingle | WANG Jing LI Xue ZHU Qinglin WANG Wenting LI Wanrong Effect of geraninn on acute lung injury induced by lipopolysaccharide in mice 陆军军医大学学报 geraninn acute lung injury lps p-nf-κbp65 |
| title | Effect of geraninn on acute lung injury induced by lipopolysaccharide in mice |
| title_full | Effect of geraninn on acute lung injury induced by lipopolysaccharide in mice |
| title_fullStr | Effect of geraninn on acute lung injury induced by lipopolysaccharide in mice |
| title_full_unstemmed | Effect of geraninn on acute lung injury induced by lipopolysaccharide in mice |
| title_short | Effect of geraninn on acute lung injury induced by lipopolysaccharide in mice |
| title_sort | effect of geraninn on acute lung injury induced by lipopolysaccharide in mice |
| topic | geraninn acute lung injury lps p-nf-κbp65 |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202204188.htm |
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