Comparative Analysis of Chemical Composition of <i>Zanthoxylum myriacanthum</i> Branches and Leaves by GC-MS and UPLC-Q-Orbitrap HRMS, and Evaluation of Their Antioxidant Activities
<i>Zanthoxylum myriacanthum</i> Wall. ex Hook. f., a plant belonging to the Rutaceae family and the <i>Zanthoxylum</i> genus, is extensively utilized for its medicinal properties and as a culinary seasoning in China and Southeast Asian countries. However, the chemical composi...
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MDPI AG
2023-07-01
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| Series: | Molecules |
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| author | Wei Dai Liangqian Zhang Liping Dai Yuan Tian Xinger Ye Sina Wang Jingtao Li Qi Wang |
| author_facet | Wei Dai Liangqian Zhang Liping Dai Yuan Tian Xinger Ye Sina Wang Jingtao Li Qi Wang |
| author_sort | Wei Dai |
| collection | DOAJ |
| description | <i>Zanthoxylum myriacanthum</i> Wall. ex Hook. f., a plant belonging to the Rutaceae family and the <i>Zanthoxylum</i> genus, is extensively utilized for its medicinal properties and as a culinary seasoning in China and Southeast Asian countries. However, the chemical composition and biological activities of <i>Z. myriacanthum</i> branches and leaves remain insufficiently explored. In this study, the volatile and non-volatile components of <i>Z. myriacanthum</i> branches and leaves were analyzed using GC-MS and UPLC-Q-Orbitrap HRMS techniques. A total of 78 volatile compounds and 66 non-volatile compounds were identified. The volatile compounds were predominantly terpenoids and aliphatic compounds, while the non-volatile compounds were primarily flavonoids and alkaloids. The branches contained 52 volatile compounds and 33 non-volatile compounds, whereas the leaves contained 48 volatile compounds and 40 non-volatile compounds. The antioxidant activities of the methanol extracts from <i>Z. myriacanthum</i> branches and leaves were evaluated using ABTS and DPPH free-radical-scavenging assays, both of which demonstrated certain antioxidant activity. The methanol extract of leaves demonstrated significantly higher antioxidant activity compared to that of the branches, possibly due to the higher presence of flavonoids and phenols in the leaves, with IC<sub>50</sub> values of 7.12 ± 0.257 μg/mL and 1.22 × 10<sup>2</sup> ± 5.01 μg/mL for ABTS and DPPH, respectively. These findings enhance our understanding of the chemical composition and antioxidant potential of <i>Z. myriacanthum</i>. The plant holds promise as a natural source of antioxidants for applications in pharmaceuticals, cosmetics, and functional foods. Further research can explore its broader biological activities and potential applications. |
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| last_indexed | 2024-03-11T00:22:15Z |
| publishDate | 2023-07-01 |
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| series | Molecules |
| spelling | doaj.art-2ff18f25602e48c580f117cbb186b5892023-11-18T23:16:47ZengMDPI AGMolecules1420-30492023-07-012815563110.3390/molecules28155631Comparative Analysis of Chemical Composition of <i>Zanthoxylum myriacanthum</i> Branches and Leaves by GC-MS and UPLC-Q-Orbitrap HRMS, and Evaluation of Their Antioxidant ActivitiesWei Dai0Liangqian Zhang1Liping Dai2Yuan Tian3Xinger Ye4Sina Wang5Jingtao Li6Qi Wang7Experimental Center of Yunfu Campus, Guangdong Pharmaceutical University, Yunfu 527325, ChinaKey Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832003, ChinaCollege of Traditional Chinese Medicine Resources, Guangdong Pharmaceutical University, Yunfu 527325, ChinaKey Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832003, ChinaCollege of Traditional Chinese Medicine Resources, Guangdong Pharmaceutical University, Yunfu 527325, ChinaExperimental Center of Yunfu Campus, Guangdong Pharmaceutical University, Yunfu 527325, ChinaExperimental Center of Yunfu Campus, Guangdong Pharmaceutical University, Yunfu 527325, ChinaKey Laboratory of Xinjiang Phytomedicine Resource and Utilization, Ministry of Education, School of Pharmacy, Shihezi University, Shihezi 832003, China<i>Zanthoxylum myriacanthum</i> Wall. ex Hook. f., a plant belonging to the Rutaceae family and the <i>Zanthoxylum</i> genus, is extensively utilized for its medicinal properties and as a culinary seasoning in China and Southeast Asian countries. However, the chemical composition and biological activities of <i>Z. myriacanthum</i> branches and leaves remain insufficiently explored. In this study, the volatile and non-volatile components of <i>Z. myriacanthum</i> branches and leaves were analyzed using GC-MS and UPLC-Q-Orbitrap HRMS techniques. A total of 78 volatile compounds and 66 non-volatile compounds were identified. The volatile compounds were predominantly terpenoids and aliphatic compounds, while the non-volatile compounds were primarily flavonoids and alkaloids. The branches contained 52 volatile compounds and 33 non-volatile compounds, whereas the leaves contained 48 volatile compounds and 40 non-volatile compounds. The antioxidant activities of the methanol extracts from <i>Z. myriacanthum</i> branches and leaves were evaluated using ABTS and DPPH free-radical-scavenging assays, both of which demonstrated certain antioxidant activity. The methanol extract of leaves demonstrated significantly higher antioxidant activity compared to that of the branches, possibly due to the higher presence of flavonoids and phenols in the leaves, with IC<sub>50</sub> values of 7.12 ± 0.257 μg/mL and 1.22 × 10<sup>2</sup> ± 5.01 μg/mL for ABTS and DPPH, respectively. These findings enhance our understanding of the chemical composition and antioxidant potential of <i>Z. myriacanthum</i>. The plant holds promise as a natural source of antioxidants for applications in pharmaceuticals, cosmetics, and functional foods. Further research can explore its broader biological activities and potential applications.https://www.mdpi.com/1420-3049/28/15/5631<i>Zanthoxylum myriacanthum</i> Wall. ex Hook. f.UPLC-Q-Orbitrap HRMSGC-MSchemical compositionantioxidant activities |
| spellingShingle | Wei Dai Liangqian Zhang Liping Dai Yuan Tian Xinger Ye Sina Wang Jingtao Li Qi Wang Comparative Analysis of Chemical Composition of <i>Zanthoxylum myriacanthum</i> Branches and Leaves by GC-MS and UPLC-Q-Orbitrap HRMS, and Evaluation of Their Antioxidant Activities Molecules <i>Zanthoxylum myriacanthum</i> Wall. ex Hook. f. UPLC-Q-Orbitrap HRMS GC-MS chemical composition antioxidant activities |
| title | Comparative Analysis of Chemical Composition of <i>Zanthoxylum myriacanthum</i> Branches and Leaves by GC-MS and UPLC-Q-Orbitrap HRMS, and Evaluation of Their Antioxidant Activities |
| title_full | Comparative Analysis of Chemical Composition of <i>Zanthoxylum myriacanthum</i> Branches and Leaves by GC-MS and UPLC-Q-Orbitrap HRMS, and Evaluation of Their Antioxidant Activities |
| title_fullStr | Comparative Analysis of Chemical Composition of <i>Zanthoxylum myriacanthum</i> Branches and Leaves by GC-MS and UPLC-Q-Orbitrap HRMS, and Evaluation of Their Antioxidant Activities |
| title_full_unstemmed | Comparative Analysis of Chemical Composition of <i>Zanthoxylum myriacanthum</i> Branches and Leaves by GC-MS and UPLC-Q-Orbitrap HRMS, and Evaluation of Their Antioxidant Activities |
| title_short | Comparative Analysis of Chemical Composition of <i>Zanthoxylum myriacanthum</i> Branches and Leaves by GC-MS and UPLC-Q-Orbitrap HRMS, and Evaluation of Their Antioxidant Activities |
| title_sort | comparative analysis of chemical composition of i zanthoxylum myriacanthum i branches and leaves by gc ms and uplc q orbitrap hrms and evaluation of their antioxidant activities |
| topic | <i>Zanthoxylum myriacanthum</i> Wall. ex Hook. f. UPLC-Q-Orbitrap HRMS GC-MS chemical composition antioxidant activities |
| url | https://www.mdpi.com/1420-3049/28/15/5631 |
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