Mycobacterium bovis: polymerase chain reaction identification in bovine Lymphonode biopsies and genotyping in isolates from Southeast Brazil by spolygotyping and restriction fragment length polymorphism

Mycobacterium bovis: polymerase chain reaction identification in bovine Lymphonode biopsies and genotyping in isolates from Southeast Brazil by spolygotyping and restriction fragment length polymorphism

Diagnosis of the Mycobacterium tuberculosis complex by direct PCR of mediastinal lymphnode DNA and microbiological tests were compared in cattle suspicious of bearing tuberculous-like lesions detected during slaughter. The PCR procedure applied on DNA samples (n=54) obtained by adding alpha -casein...

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Bibliographic Details
Main Authors: Zanini MS, Moreira EC, Lopes MTP, Oliveira RS, Leão SC, Fioravanti RL, Roxo E, Zumarraga M, Romano MI, Cataldi A, Salas CE
Format: Article
Language:English
Published: Fundação Oswaldo Cruz (FIOCRUZ) 2001-01-01
Series:Memorias do Instituto Oswaldo Cruz
Subjects:
Mycobacterium bovis diagnosis
DNA polimorphism
thiocyanate guanidinium
Brazil
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762001000600012

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