The deletion of omp19 gene of brucella abortus 2308 reduces its survival in mouse macrophage and in mice
The aim of this study is to investigate the role of outer membrane protein Omp19 of Brucella in its pathogenesis. In this research, the Brucella 2308 ΔOmp19 deletion mutant was constructed and its intracellular survivability was evaluated in murine macrophages RAW264.7 and BALB/c mice. We also analy...
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| Format: | Article |
| Language: | English |
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Kafkas University, Faculty of Veterinary Medicine
2020-07-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
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| Online Access: | https://vetdergikafkas.org/pdf.php?id=2731 |
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| author | Yueli WANG Zhongchen MA Huan ZHANG Jihai YI Yong WANG Tiansen LI Chuangfu CHEN |
| author_facet | Yueli WANG Zhongchen MA Huan ZHANG Jihai YI Yong WANG Tiansen LI Chuangfu CHEN |
| author_sort | Yueli WANG |
| collection | DOAJ |
| description | The aim of this study is to investigate the role of outer membrane protein Omp19 of Brucella in its pathogenesis. In this research, the Brucella
2308 ΔOmp19 deletion mutant was constructed and its intracellular survivability was evaluated in murine macrophages RAW264.7 and
BALB/c mice. We also analyzed the lysosomal fusion induced by and cytotoxicity of Brucella in murine macrophages RAW264.7. The results
showed that the Brucella 2308 ΔOmp19 induced higher levels of immunoprotective cytokines in vitro than Brucella 2308, and the number of
intracellular bacteria in RAW264.7 macrophages was lower after Brucella 2308 ΔOmp19 infection than after Brucella 2308 infection. In vivo,
Brucella 2308 ΔOmp19 induced protective immune response in mice, and the splenic bacterial load of this deletion mutant was lower than
that of Brucella 2308. In addition, the Brucella 2308 ΔOmp19 also promoted the fusion of Brucella-containing vacuoles and lysosomes, in
macrophages. Therefore, Brucella 2308 ΔOmp19 reduced virulence compared with Brucella 2308 and activate the immune response of the
host, and is a promising candidate for a live attenuated vaccine. |
| first_indexed | 2024-03-13T04:39:08Z |
| format | Article |
| id | doaj.art-2ff6107985a8443fa5e298135f02bb60 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:39:08Z |
| publishDate | 2020-07-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-2ff6107985a8443fa5e298135f02bb602023-06-19T06:32:21ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512020-07-0126674975510.9775/kvfd.2020.242802731The deletion of omp19 gene of brucella abortus 2308 reduces its survival in mouse macrophage and in miceYueli WANG0Zhongchen MA1Huan ZHANG2Jihai YI3Yong WANG4Tiansen LI5Chuangfu CHEN6College of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, CHINACollege of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, CHINACollege of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, CHINACollege of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, CHINACollege of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, CHINACollege of Animal Science and Technology, Hainan University, 570228, Haikou, Hainan, CHINACollege of Animal Science and Technology, Shihezi University, 832000, Shihezi, Xinjiang, CHINAThe aim of this study is to investigate the role of outer membrane protein Omp19 of Brucella in its pathogenesis. In this research, the Brucella 2308 ΔOmp19 deletion mutant was constructed and its intracellular survivability was evaluated in murine macrophages RAW264.7 and BALB/c mice. We also analyzed the lysosomal fusion induced by and cytotoxicity of Brucella in murine macrophages RAW264.7. The results showed that the Brucella 2308 ΔOmp19 induced higher levels of immunoprotective cytokines in vitro than Brucella 2308, and the number of intracellular bacteria in RAW264.7 macrophages was lower after Brucella 2308 ΔOmp19 infection than after Brucella 2308 infection. In vivo, Brucella 2308 ΔOmp19 induced protective immune response in mice, and the splenic bacterial load of this deletion mutant was lower than that of Brucella 2308. In addition, the Brucella 2308 ΔOmp19 also promoted the fusion of Brucella-containing vacuoles and lysosomes, in macrophages. Therefore, Brucella 2308 ΔOmp19 reduced virulence compared with Brucella 2308 and activate the immune response of the host, and is a promising candidate for a live attenuated vaccine.https://vetdergikafkas.org/pdf.php?id=2731brucellaomp19 genehomologous recombinationviabilityintracellular survival |
| spellingShingle | Yueli WANG Zhongchen MA Huan ZHANG Jihai YI Yong WANG Tiansen LI Chuangfu CHEN The deletion of omp19 gene of brucella abortus 2308 reduces its survival in mouse macrophage and in mice Kafkas Universitesi Veteriner Fakültesi Dergisi brucella omp19 gene homologous recombination viability intracellular survival |
| title | The deletion of omp19 gene of brucella abortus 2308 reduces its survival in mouse macrophage and in mice |
| title_full | The deletion of omp19 gene of brucella abortus 2308 reduces its survival in mouse macrophage and in mice |
| title_fullStr | The deletion of omp19 gene of brucella abortus 2308 reduces its survival in mouse macrophage and in mice |
| title_full_unstemmed | The deletion of omp19 gene of brucella abortus 2308 reduces its survival in mouse macrophage and in mice |
| title_short | The deletion of omp19 gene of brucella abortus 2308 reduces its survival in mouse macrophage and in mice |
| title_sort | deletion of omp19 gene of brucella abortus 2308 reduces its survival in mouse macrophage and in mice |
| topic | brucella omp19 gene homologous recombination viability intracellular survival |
| url | https://vetdergikafkas.org/pdf.php?id=2731 |
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