A 90-Day Safety Study of Meat from <i>MSTN</i> and <i>FGF5</i> Double-Knockout Sheep in Wistar Rats
<i>MSTN</i> and <i>FGF5</i> gene knockout sheep generated by the CRISPR/Cas9 system exhibit the ‘double-muscle’ phenotype, and increased density and length of hairs, providing valuable new breeding material. In a previous study, we obtained <i>MSTN</i> and <i&g...
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MDPI AG
2022-01-01
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| author | Yue Zhao Mingming Chen Yao Li Xueling Xu Sujun Wu Zhimei Liu Shiyu Qi Guang Yi Xiaosheng Zhang Jinlong Zhang Xiaofei Guo Kun Yu Shoulong Deng Yan Li Zhengxing Lian |
| author_facet | Yue Zhao Mingming Chen Yao Li Xueling Xu Sujun Wu Zhimei Liu Shiyu Qi Guang Yi Xiaosheng Zhang Jinlong Zhang Xiaofei Guo Kun Yu Shoulong Deng Yan Li Zhengxing Lian |
| author_sort | Yue Zhao |
| collection | DOAJ |
| description | <i>MSTN</i> and <i>FGF5</i> gene knockout sheep generated by the CRISPR/Cas9 system exhibit the ‘double-muscle’ phenotype, and increased density and length of hairs, providing valuable new breeding material. In a previous study, we obtained <i>MSTN</i> and <i>FGF5</i> double-knockout sheep of significant breeding value. In this study, we carried out a 90-day feeding study in Wistar rats to assess the safety of genome-edited mutton. Seven rat groups with 10 females and 10 males per group were fed different concentrations (3.75%, 7.5%, and 15%) of double-knockout mutton or wild-type mutton in a conventional commercial diet for 90 days. At the end of the feeding, routine urine and blood tests and measurements of blood biochemical indicators were performed. Furthermore, the major organs of each group of rats were weighed and examined histopathologically. Although there were significant differences among the groups in some parameters, all values were within the normal ranges. Therefore, the 90-day rat feeding study showed that the meat from <i>MSTN</i> and <i>FGF5</i> double-knockout sheep did not have any long-term adverse effects on rat health. This study also provides valuable reference information for assessing the safety of meat from animals with knockout of multiple genes. |
| first_indexed | 2024-03-09T21:35:06Z |
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| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-09T21:35:06Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-2ff6effc96734289b47a856fff357f722023-11-23T20:45:45ZengMDPI AGLife2075-17292022-01-0112220410.3390/life12020204A 90-Day Safety Study of Meat from <i>MSTN</i> and <i>FGF5</i> Double-Knockout Sheep in Wistar RatsYue Zhao0Mingming Chen1Yao Li2Xueling Xu3Sujun Wu4Zhimei Liu5Shiyu Qi6Guang Yi7Xiaosheng Zhang8Jinlong Zhang9Xiaofei Guo10Kun Yu11Shoulong Deng12Yan Li13Zhengxing Lian14Beijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Tianjin Academy of Agricultural Sciences, Tianjin 300381, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Tianjin Academy of Agricultural Sciences, Tianjin 300381, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Tianjin Academy of Agricultural Sciences, Tianjin 300381, ChinaBeijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, ChinaBeijing Key Laboratory for Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing 100193, China<i>MSTN</i> and <i>FGF5</i> gene knockout sheep generated by the CRISPR/Cas9 system exhibit the ‘double-muscle’ phenotype, and increased density and length of hairs, providing valuable new breeding material. In a previous study, we obtained <i>MSTN</i> and <i>FGF5</i> double-knockout sheep of significant breeding value. In this study, we carried out a 90-day feeding study in Wistar rats to assess the safety of genome-edited mutton. Seven rat groups with 10 females and 10 males per group were fed different concentrations (3.75%, 7.5%, and 15%) of double-knockout mutton or wild-type mutton in a conventional commercial diet for 90 days. At the end of the feeding, routine urine and blood tests and measurements of blood biochemical indicators were performed. Furthermore, the major organs of each group of rats were weighed and examined histopathologically. Although there were significant differences among the groups in some parameters, all values were within the normal ranges. Therefore, the 90-day rat feeding study showed that the meat from <i>MSTN</i> and <i>FGF5</i> double-knockout sheep did not have any long-term adverse effects on rat health. This study also provides valuable reference information for assessing the safety of meat from animals with knockout of multiple genes.https://www.mdpi.com/2075-1729/12/2/204meat<i>MSTN</i><i>FGF5</i>sheep90-day feedingrat |
| spellingShingle | Yue Zhao Mingming Chen Yao Li Xueling Xu Sujun Wu Zhimei Liu Shiyu Qi Guang Yi Xiaosheng Zhang Jinlong Zhang Xiaofei Guo Kun Yu Shoulong Deng Yan Li Zhengxing Lian A 90-Day Safety Study of Meat from <i>MSTN</i> and <i>FGF5</i> Double-Knockout Sheep in Wistar Rats Life meat <i>MSTN</i> <i>FGF5</i> sheep 90-day feeding rat |
| title | A 90-Day Safety Study of Meat from <i>MSTN</i> and <i>FGF5</i> Double-Knockout Sheep in Wistar Rats |
| title_full | A 90-Day Safety Study of Meat from <i>MSTN</i> and <i>FGF5</i> Double-Knockout Sheep in Wistar Rats |
| title_fullStr | A 90-Day Safety Study of Meat from <i>MSTN</i> and <i>FGF5</i> Double-Knockout Sheep in Wistar Rats |
| title_full_unstemmed | A 90-Day Safety Study of Meat from <i>MSTN</i> and <i>FGF5</i> Double-Knockout Sheep in Wistar Rats |
| title_short | A 90-Day Safety Study of Meat from <i>MSTN</i> and <i>FGF5</i> Double-Knockout Sheep in Wistar Rats |
| title_sort | 90 day safety study of meat from i mstn i and i fgf5 i double knockout sheep in wistar rats |
| topic | meat <i>MSTN</i> <i>FGF5</i> sheep 90-day feeding rat |
| url | https://www.mdpi.com/2075-1729/12/2/204 |
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