Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity
Beneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in formulations towards the prevention of respiratory viral infections. In this study, novel immunobiotic strains with the ability to increase antiviral i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-09-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/11/19/2986 |
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| author | Mikado Tomokiyo Fernanda Raya Tonetti Hikari Yamamuro Ryoko Shibata Kohtaro Fukuyama Nadia Gobbato Leonardo Albarracin Muhammad Shahid Riaz Rajoka A. K. M. Humayun Kober Wakako Ikeda-Ohtsubo Julio Villena Haruki Kitazawa |
| author_facet | Mikado Tomokiyo Fernanda Raya Tonetti Hikari Yamamuro Ryoko Shibata Kohtaro Fukuyama Nadia Gobbato Leonardo Albarracin Muhammad Shahid Riaz Rajoka A. K. M. Humayun Kober Wakako Ikeda-Ohtsubo Julio Villena Haruki Kitazawa |
| author_sort | Mikado Tomokiyo |
| collection | DOAJ |
| description | Beneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in formulations towards the prevention of respiratory viral infections. In this study, novel immunobiotic strains with the ability to increase antiviral immunity in porcine alveolar macrophages were selected from a library of <i>Lactobacillus gasseri</i>. Postimmunobiotics derived from the most remarkable strains were also evaluated in their capacity to modulate the immune response triggered by Toll-like receptor 3 (TLR3) in alveolar macrophages and to differentially regulate TLR3-mediated antiviral respiratory immunity in infant mice. We provide evidence that porcine alveolar macrophages (3D4/31 cells) are a useful in vitro tool for the screening of new antiviral immunobiotics and postimmunobiotics by assessing their ability to modulate the expression <i>IFN-β</i>, <i>IFN-λ1</i>, <i>RNAseL</i>, <i>Mx2</i>, and <i>IL-6</i>, which can be used as prospective biomarkers. We also demonstrate that the postimmunobiotics derived from the <i>Lactobacillus gasseri</i> TMT36, TMT39 and TMT40 (HK36, HK39 or HK40) strains modulate the innate antiviral immune response of alveolar macrophages and reduce lung inflammatory damage triggered by TLR3 activation in vivo. Although our findings should be deepened and expanded, the results of the present work provide a scientific rationale for the use of nasally administered HK36, HK39 or HK40 to beneficially modulate TLR3-triggerd respiratory innate immune response. |
| first_indexed | 2024-03-09T21:54:34Z |
| format | Article |
| id | doaj.art-2ff7a67699f94eb3bc1fc79eac1c0470 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-09T21:54:34Z |
| publishDate | 2022-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-2ff7a67699f94eb3bc1fc79eac1c04702023-11-23T20:00:39ZengMDPI AGCells2073-44092022-09-011119298610.3390/cells11192986Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory ImmunityMikado Tomokiyo0Fernanda Raya Tonetti1Hikari Yamamuro2Ryoko Shibata3Kohtaro Fukuyama4Nadia Gobbato5Leonardo Albarracin6Muhammad Shahid Riaz Rajoka7A. K. M. Humayun Kober8Wakako Ikeda-Ohtsubo9Julio Villena10Haruki Kitazawa11Food and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8576, JapanLaboratory of Immunobiotechnology, Reference Centre for Lactobacilli CERELA-CONICET, San Miguel de Tucuman, Tucuman CP4000, ArgentinaFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8576, JapanFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8576, JapanFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8576, JapanLaboratory of Immunology, Microbiology Institute, Faculty of Biochemestry, Chemestry and Pharmacy, National University of Tucuman, Tucuman CP4000, ArgentinaFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8576, JapanFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8576, JapanFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8576, JapanFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8576, JapanFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8576, JapanFood and Feed Immunology Group, Laboratory of Animal Food Function, Graduate School of Agricultural Science, Tohoku University, Sendai 980-8576, JapanBeneficial microbes with immunomodulatory capacities (immunobiotics) and their non-viable forms (postimmunobiotics) could be effectively utilized in formulations towards the prevention of respiratory viral infections. In this study, novel immunobiotic strains with the ability to increase antiviral immunity in porcine alveolar macrophages were selected from a library of <i>Lactobacillus gasseri</i>. Postimmunobiotics derived from the most remarkable strains were also evaluated in their capacity to modulate the immune response triggered by Toll-like receptor 3 (TLR3) in alveolar macrophages and to differentially regulate TLR3-mediated antiviral respiratory immunity in infant mice. We provide evidence that porcine alveolar macrophages (3D4/31 cells) are a useful in vitro tool for the screening of new antiviral immunobiotics and postimmunobiotics by assessing their ability to modulate the expression <i>IFN-β</i>, <i>IFN-λ1</i>, <i>RNAseL</i>, <i>Mx2</i>, and <i>IL-6</i>, which can be used as prospective biomarkers. We also demonstrate that the postimmunobiotics derived from the <i>Lactobacillus gasseri</i> TMT36, TMT39 and TMT40 (HK36, HK39 or HK40) strains modulate the innate antiviral immune response of alveolar macrophages and reduce lung inflammatory damage triggered by TLR3 activation in vivo. Although our findings should be deepened and expanded, the results of the present work provide a scientific rationale for the use of nasally administered HK36, HK39 or HK40 to beneficially modulate TLR3-triggerd respiratory innate immune response.https://www.mdpi.com/2073-4409/11/19/2986porcine alveolar macrophagesTLR3immunobioticspostimmunobiotics<i>Lactobacillus gasseri</i>lung inflammatory damage |
| spellingShingle | Mikado Tomokiyo Fernanda Raya Tonetti Hikari Yamamuro Ryoko Shibata Kohtaro Fukuyama Nadia Gobbato Leonardo Albarracin Muhammad Shahid Riaz Rajoka A. K. M. Humayun Kober Wakako Ikeda-Ohtsubo Julio Villena Haruki Kitazawa Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity Cells porcine alveolar macrophages TLR3 immunobiotics postimmunobiotics <i>Lactobacillus gasseri</i> lung inflammatory damage |
| title | Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity |
| title_full | Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity |
| title_fullStr | Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity |
| title_full_unstemmed | Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity |
| title_short | Modulation of Alveolar Macrophages by Postimmunobiotics: Impact on TLR3-Mediated Antiviral Respiratory Immunity |
| title_sort | modulation of alveolar macrophages by postimmunobiotics impact on tlr3 mediated antiviral respiratory immunity |
| topic | porcine alveolar macrophages TLR3 immunobiotics postimmunobiotics <i>Lactobacillus gasseri</i> lung inflammatory damage |
| url | https://www.mdpi.com/2073-4409/11/19/2986 |
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