The Quantitative Detection of Cystatin-C in Patient Samples Using a Colorimetric Lateral Flow Immunoassay
A colloidal gold-based lateral flow immunoassay was developed for the rapid quantitative detection of Cystatin-C in serum and whole blood. This device has an assay time of 15 min, making it a convenient point-of-care diagnostic tool. The device has a quantification range spanning from 0.5 to 7.5 µg/...
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| Format: | Article |
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MDPI AG
2024-01-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/14/1/30 |
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| author | Santosh Kumar Bikkarolla Kavipriya Venkatesan Yeddula Rebecca Revathy Sowmya Parameswaran Subramanian Krishnakumar Dhananjaya Dendukuri |
| author_facet | Santosh Kumar Bikkarolla Kavipriya Venkatesan Yeddula Rebecca Revathy Sowmya Parameswaran Subramanian Krishnakumar Dhananjaya Dendukuri |
| author_sort | Santosh Kumar Bikkarolla |
| collection | DOAJ |
| description | A colloidal gold-based lateral flow immunoassay was developed for the rapid quantitative detection of Cystatin-C in serum and whole blood. This device has an assay time of 15 min, making it a convenient point-of-care diagnostic tool. The device has a quantification range spanning from 0.5 to 7.5 µg/mL, with a lower limit of detection at 0.18 µg/mL. To validate its accuracy, the test was compared to a standard nephelometric immunoassay, and the results exhibited a robust linear correlation with an adjusted r<sup>2</sup> value of 0.95. Furthermore, the device demonstrates satisfactory levels of analytical performance in terms of precision, sensitivity, and interference, indicating its potential for precise Cystatin-C quantification, particularly in renal-failure patients. Notably, the Cystatin-C-LFA device also demonstrates satisfactory stability, as a 30-day accelerated stability study at 50 °C showed no change in the device performance, indicating a long shelf life for the product when stored at room temperature. |
| first_indexed | 2024-03-08T11:03:04Z |
| format | Article |
| id | doaj.art-2ffe16f0804948389f164d08d8f2c4ce |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-08T11:03:04Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-2ffe16f0804948389f164d08d8f2c4ce2024-01-26T15:23:09ZengMDPI AGBiosensors2079-63742024-01-011413010.3390/bios14010030The Quantitative Detection of Cystatin-C in Patient Samples Using a Colorimetric Lateral Flow ImmunoassaySantosh Kumar Bikkarolla0Kavipriya Venkatesan1Yeddula Rebecca Revathy2Sowmya Parameswaran3Subramanian Krishnakumar4Dhananjaya Dendukuri5Achira Labs, 66b, 13th Cross Rd, Dollar Layout, 3rd Phase, J. P. Nagar, Bengaluru 560078, IndiaAchira Labs, 66b, 13th Cross Rd, Dollar Layout, 3rd Phase, J. P. Nagar, Bengaluru 560078, IndiaVision Research Foundation, Chennai 600006, IndiaVision Research Foundation, Chennai 600006, IndiaVision Research Foundation, Chennai 600006, IndiaAchira Labs, 66b, 13th Cross Rd, Dollar Layout, 3rd Phase, J. P. Nagar, Bengaluru 560078, IndiaA colloidal gold-based lateral flow immunoassay was developed for the rapid quantitative detection of Cystatin-C in serum and whole blood. This device has an assay time of 15 min, making it a convenient point-of-care diagnostic tool. The device has a quantification range spanning from 0.5 to 7.5 µg/mL, with a lower limit of detection at 0.18 µg/mL. To validate its accuracy, the test was compared to a standard nephelometric immunoassay, and the results exhibited a robust linear correlation with an adjusted r<sup>2</sup> value of 0.95. Furthermore, the device demonstrates satisfactory levels of analytical performance in terms of precision, sensitivity, and interference, indicating its potential for precise Cystatin-C quantification, particularly in renal-failure patients. Notably, the Cystatin-C-LFA device also demonstrates satisfactory stability, as a 30-day accelerated stability study at 50 °C showed no change in the device performance, indicating a long shelf life for the product when stored at room temperature.https://www.mdpi.com/2079-6374/14/1/30gold nanoparticleslateral flow immunoassayCystatin-Cpatient-whole bloodserum-samples |
| spellingShingle | Santosh Kumar Bikkarolla Kavipriya Venkatesan Yeddula Rebecca Revathy Sowmya Parameswaran Subramanian Krishnakumar Dhananjaya Dendukuri The Quantitative Detection of Cystatin-C in Patient Samples Using a Colorimetric Lateral Flow Immunoassay Biosensors gold nanoparticles lateral flow immunoassay Cystatin-C patient-whole blood serum-samples |
| title | The Quantitative Detection of Cystatin-C in Patient Samples Using a Colorimetric Lateral Flow Immunoassay |
| title_full | The Quantitative Detection of Cystatin-C in Patient Samples Using a Colorimetric Lateral Flow Immunoassay |
| title_fullStr | The Quantitative Detection of Cystatin-C in Patient Samples Using a Colorimetric Lateral Flow Immunoassay |
| title_full_unstemmed | The Quantitative Detection of Cystatin-C in Patient Samples Using a Colorimetric Lateral Flow Immunoassay |
| title_short | The Quantitative Detection of Cystatin-C in Patient Samples Using a Colorimetric Lateral Flow Immunoassay |
| title_sort | quantitative detection of cystatin c in patient samples using a colorimetric lateral flow immunoassay |
| topic | gold nanoparticles lateral flow immunoassay Cystatin-C patient-whole blood serum-samples |
| url | https://www.mdpi.com/2079-6374/14/1/30 |
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