Transcriptome Profiling of Potato (<i>Solanum tuberosum</i> L.) Responses to Root-Knot Nematode (<i>Meloidogyne javanica</i>) Infestation during A Compatible Interaction
Root-knot nematode (RKN) <i>Meloidogyne javanica</i> presents a great challenge to Solanaceae crops, including potato. In this study, we investigated transcriptional responses of potato roots during a compatible interaction with <i>M. javanica</i>. In this respect, differenti...
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MDPI AG
2020-09-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/8/9/1443 |
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| author | Teresia N. Macharia Daniel Bellieny-Rabelo Lucy N. Moleleki |
| author_facet | Teresia N. Macharia Daniel Bellieny-Rabelo Lucy N. Moleleki |
| author_sort | Teresia N. Macharia |
| collection | DOAJ |
| description | Root-knot nematode (RKN) <i>Meloidogyne javanica</i> presents a great challenge to Solanaceae crops, including potato. In this study, we investigated transcriptional responses of potato roots during a compatible interaction with <i>M. javanica</i>. In this respect, differential gene expression of <i>Solanum tuberosum</i> cultivar (cv.) Mondial challenged with <i>M. javanica</i> at 0, 3 and 7 days post-inoculation (dpi) was profiled. In total, 4948 and 4484 genes were detected, respectively, as differentially expressed genes (DEGs) at 3 and 7 dpi. Functional annotation revealed that genes associated with metabolic processes were enriched, suggesting they might have an important role in <i>M. javanica</i> disease development. MapMan analysis revealed down-regulation of genes associated with pathogen perception and signaling suggesting interference with plant immunity system. Notably, delayed activation of pathogenesis-related genes, down-regulation of disease resistance genes, and activation of host antioxidant system contributed to a susceptible response. Nematode infestation suppressed ethylene (ET) and jasmonic acid (JA) signaling pathway hindering JA/ET responsive genes associated with defense. Genes related to cell wall modification were differentially regulated while transport-related genes were up-regulated, facilitating the formation of nematode feeding sites (NFSs). Several families of transcription factors (TFs) were differentially regulated by <i>M. javanica</i> infestation. Suggesting that TFs play an indispensable role in physiological adaptation for successful <i>M. javanica</i> disease development. This genome-wide analysis reveals the molecular regulatory networks in potato roots which are potentially manipulated by <i>M. javanica</i>. Being the first study analyzing transcriptome profiling of <i>M. javanica</i>-diseased potato, it provides unparalleled insight into the mechanism underlying disease development. |
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| series | Microorganisms |
| spelling | doaj.art-3000b0f50b2c43efb43446e17add17fa2023-11-20T14:27:17ZengMDPI AGMicroorganisms2076-26072020-09-0189144310.3390/microorganisms8091443Transcriptome Profiling of Potato (<i>Solanum tuberosum</i> L.) Responses to Root-Knot Nematode (<i>Meloidogyne javanica</i>) Infestation during A Compatible InteractionTeresia N. Macharia0Daniel Bellieny-Rabelo1Lucy N. Moleleki2Department of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0002, South AfricaDepartment of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0002, South AfricaDepartment of Biochemistry, Genetics and Microbiology, Forestry and Agricultural Biotechnology Institute, University of Pretoria, Pretoria 0002, South AfricaRoot-knot nematode (RKN) <i>Meloidogyne javanica</i> presents a great challenge to Solanaceae crops, including potato. In this study, we investigated transcriptional responses of potato roots during a compatible interaction with <i>M. javanica</i>. In this respect, differential gene expression of <i>Solanum tuberosum</i> cultivar (cv.) Mondial challenged with <i>M. javanica</i> at 0, 3 and 7 days post-inoculation (dpi) was profiled. In total, 4948 and 4484 genes were detected, respectively, as differentially expressed genes (DEGs) at 3 and 7 dpi. Functional annotation revealed that genes associated with metabolic processes were enriched, suggesting they might have an important role in <i>M. javanica</i> disease development. MapMan analysis revealed down-regulation of genes associated with pathogen perception and signaling suggesting interference with plant immunity system. Notably, delayed activation of pathogenesis-related genes, down-regulation of disease resistance genes, and activation of host antioxidant system contributed to a susceptible response. Nematode infestation suppressed ethylene (ET) and jasmonic acid (JA) signaling pathway hindering JA/ET responsive genes associated with defense. Genes related to cell wall modification were differentially regulated while transport-related genes were up-regulated, facilitating the formation of nematode feeding sites (NFSs). Several families of transcription factors (TFs) were differentially regulated by <i>M. javanica</i> infestation. Suggesting that TFs play an indispensable role in physiological adaptation for successful <i>M. javanica</i> disease development. This genome-wide analysis reveals the molecular regulatory networks in potato roots which are potentially manipulated by <i>M. javanica</i>. Being the first study analyzing transcriptome profiling of <i>M. javanica</i>-diseased potato, it provides unparalleled insight into the mechanism underlying disease development.https://www.mdpi.com/2076-2607/8/9/1443root-knot nematode<i>Solanum tuberosum</i>differentially expressed genesdefense response |
| spellingShingle | Teresia N. Macharia Daniel Bellieny-Rabelo Lucy N. Moleleki Transcriptome Profiling of Potato (<i>Solanum tuberosum</i> L.) Responses to Root-Knot Nematode (<i>Meloidogyne javanica</i>) Infestation during A Compatible Interaction Microorganisms root-knot nematode <i>Solanum tuberosum</i> differentially expressed genes defense response |
| title | Transcriptome Profiling of Potato (<i>Solanum tuberosum</i> L.) Responses to Root-Knot Nematode (<i>Meloidogyne javanica</i>) Infestation during A Compatible Interaction |
| title_full | Transcriptome Profiling of Potato (<i>Solanum tuberosum</i> L.) Responses to Root-Knot Nematode (<i>Meloidogyne javanica</i>) Infestation during A Compatible Interaction |
| title_fullStr | Transcriptome Profiling of Potato (<i>Solanum tuberosum</i> L.) Responses to Root-Knot Nematode (<i>Meloidogyne javanica</i>) Infestation during A Compatible Interaction |
| title_full_unstemmed | Transcriptome Profiling of Potato (<i>Solanum tuberosum</i> L.) Responses to Root-Knot Nematode (<i>Meloidogyne javanica</i>) Infestation during A Compatible Interaction |
| title_short | Transcriptome Profiling of Potato (<i>Solanum tuberosum</i> L.) Responses to Root-Knot Nematode (<i>Meloidogyne javanica</i>) Infestation during A Compatible Interaction |
| title_sort | transcriptome profiling of potato i solanum tuberosum i l responses to root knot nematode i meloidogyne javanica i infestation during a compatible interaction |
| topic | root-knot nematode <i>Solanum tuberosum</i> differentially expressed genes defense response |
| url | https://www.mdpi.com/2076-2607/8/9/1443 |
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