Identification of 5 novel feline erythrocyte antigens based on the presence of naturally occurring alloantibodies
Abstract Background Since the discovery of the Mik antigen, several studies have described blood incompatibilities unrelated to the AB system in cats. Objective To estimate the prevalence of cats with non‐AB incompatibilities associated with naturally occurring alloantibodies (NOAb), and to begin ma...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2021-01-01
|
| Series: | Journal of Veterinary Internal Medicine |
| Subjects: | |
| Online Access: | https://doi.org/10.1111/jvim.16010 |
| _version_ | 1828219492717559808 |
|---|---|
| author | Marie Binvel Julie Arsenault Boris Depré Marie‐Claude Blais |
| author_facet | Marie Binvel Julie Arsenault Boris Depré Marie‐Claude Blais |
| author_sort | Marie Binvel |
| collection | DOAJ |
| description | Abstract Background Since the discovery of the Mik antigen, several studies have described blood incompatibilities unrelated to the AB system in cats. Objective To estimate the prevalence of cats with non‐AB incompatibilities associated with naturally occurring alloantibodies (NOAb), and to begin mapping the corresponding feline erythrocyte antigens (FEA). Animals Two hundred and fifty‐eight type A cats. Methods Prospectively, cats were evaluated for the presence of NOAb by crossmatching in groups of 4‐6 cats. When NOAb were detected in a cat, its plasma was used as reagent to assess for the presence of the corresponding FEA in all cats included thereafter, and agreement observed between results of this extensive blood typing was evaluated. Results The chance of detecting incompatibilities by randomly crossmatching 2 cats was 3.9%, which resulted in at least 7% of type A cats having NOAb. Blood typing and agreement analyses performed with 7 newly detected NOAb allowed the identification of 5 presumably distinct FEA. Feline erythrocyte antigens 1 and 5 were most frequent with prevalence of 84% and 96%, respectively. Only FEA 1‐negative status was associated with a higher risk of presenting NOAb; with 16.7% of 42 FEA 1‐negative cats having NOAb compared to 5.1% of 216 FEA 1‐positive cats. Conclusions and Clinical Importance This study represents a first step of FEA identification outside the AB system. Because of its prevalence and association with NOAb, FEA 1 might correspond to the Mik antigen. |
| first_indexed | 2024-04-12T16:17:11Z |
| format | Article |
| id | doaj.art-3004431d7ab04c5dae661bcc93853b9d |
| institution | Directory Open Access Journal |
| issn | 0891-6640 1939-1676 |
| language | English |
| last_indexed | 2024-04-12T16:17:11Z |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Veterinary Internal Medicine |
| spelling | doaj.art-3004431d7ab04c5dae661bcc93853b9d2022-12-22T03:25:42ZengWileyJournal of Veterinary Internal Medicine0891-66401939-16762021-01-0135123424410.1111/jvim.16010Identification of 5 novel feline erythrocyte antigens based on the presence of naturally occurring alloantibodiesMarie Binvel0Julie Arsenault1Boris Depré2Marie‐Claude Blais3Department of Clinical Sciences, Faculty of Veterinary Medicine Université de Montréal Saint‐Hyacinthe CanadaDepartment of Pathology and Microbiology, Faculty of Veterinary Medicine Université de Montréal Saint‐Hyacinthe CanadaEmergency and Critical Care, Adomvet Lyon FranceDepartment of Clinical Sciences, Faculty of Veterinary Medicine Université de Montréal Saint‐Hyacinthe CanadaAbstract Background Since the discovery of the Mik antigen, several studies have described blood incompatibilities unrelated to the AB system in cats. Objective To estimate the prevalence of cats with non‐AB incompatibilities associated with naturally occurring alloantibodies (NOAb), and to begin mapping the corresponding feline erythrocyte antigens (FEA). Animals Two hundred and fifty‐eight type A cats. Methods Prospectively, cats were evaluated for the presence of NOAb by crossmatching in groups of 4‐6 cats. When NOAb were detected in a cat, its plasma was used as reagent to assess for the presence of the corresponding FEA in all cats included thereafter, and agreement observed between results of this extensive blood typing was evaluated. Results The chance of detecting incompatibilities by randomly crossmatching 2 cats was 3.9%, which resulted in at least 7% of type A cats having NOAb. Blood typing and agreement analyses performed with 7 newly detected NOAb allowed the identification of 5 presumably distinct FEA. Feline erythrocyte antigens 1 and 5 were most frequent with prevalence of 84% and 96%, respectively. Only FEA 1‐negative status was associated with a higher risk of presenting NOAb; with 16.7% of 42 FEA 1‐negative cats having NOAb compared to 5.1% of 216 FEA 1‐positive cats. Conclusions and Clinical Importance This study represents a first step of FEA identification outside the AB system. Because of its prevalence and association with NOAb, FEA 1 might correspond to the Mik antigen.https://doi.org/10.1111/jvim.16010alloimmunisationblood compatibilityblood typingcrossmatchMik antigentransfusion |
| spellingShingle | Marie Binvel Julie Arsenault Boris Depré Marie‐Claude Blais Identification of 5 novel feline erythrocyte antigens based on the presence of naturally occurring alloantibodies Journal of Veterinary Internal Medicine alloimmunisation blood compatibility blood typing crossmatch Mik antigen transfusion |
| title | Identification of 5 novel feline erythrocyte antigens based on the presence of naturally occurring alloantibodies |
| title_full | Identification of 5 novel feline erythrocyte antigens based on the presence of naturally occurring alloantibodies |
| title_fullStr | Identification of 5 novel feline erythrocyte antigens based on the presence of naturally occurring alloantibodies |
| title_full_unstemmed | Identification of 5 novel feline erythrocyte antigens based on the presence of naturally occurring alloantibodies |
| title_short | Identification of 5 novel feline erythrocyte antigens based on the presence of naturally occurring alloantibodies |
| title_sort | identification of 5 novel feline erythrocyte antigens based on the presence of naturally occurring alloantibodies |
| topic | alloimmunisation blood compatibility blood typing crossmatch Mik antigen transfusion |
| url | https://doi.org/10.1111/jvim.16010 |
| work_keys_str_mv | AT mariebinvel identificationof5novelfelineerythrocyteantigensbasedonthepresenceofnaturallyoccurringalloantibodies AT juliearsenault identificationof5novelfelineerythrocyteantigensbasedonthepresenceofnaturallyoccurringalloantibodies AT borisdepre identificationof5novelfelineerythrocyteantigensbasedonthepresenceofnaturallyoccurringalloantibodies AT marieclaudeblais identificationof5novelfelineerythrocyteantigensbasedonthepresenceofnaturallyoccurringalloantibodies |