Molecular Epidemiology of Human Herpes Virus Type 8 Among Patients with Compromised Immune System in Ouagadougou, Burkina Faso
Nakougou Mo&idigr;-bohm Biatougou,1 Muriel S Ouedraogo,2 Serge Theophile Soubeiga,3,4 Theodora Mahoukede Zohoncon,1,5 Paul Ouedraogo,5 Dorcas Obiri-Yeboah,6 Aziz Sidi Aristide Tapsoba,1 Touwendpoulimdé Isabelle Kiendrebeogo,1,3 Tani Sagna,4 Pascal Niamba,2 Adama Traore,2 Jacques Simpore1,3 1Bioc...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2022-07-01
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| Series: | HIV/AIDS: Research and Palliative Care |
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| Online Access: | https://www.dovepress.com/molecular-epidemiology-of-human-herpes-virus-type-8-among-patients-wit-peer-reviewed-fulltext-article-HIV |
| _version_ | 1828516831680266240 |
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| author | Biatougou NMB Ouedraogo MS Soubeiga ST Zohoncon TM Ouedraogo P Obiri-Yeboah D Tapsoba ASA Kiendrebeogo TI Sagna T Niamba P Traore A Simpore J |
| author_facet | Biatougou NMB Ouedraogo MS Soubeiga ST Zohoncon TM Ouedraogo P Obiri-Yeboah D Tapsoba ASA Kiendrebeogo TI Sagna T Niamba P Traore A Simpore J |
| author_sort | Biatougou NMB |
| collection | DOAJ |
| description | Nakougou Mo&idigr;-bohm Biatougou,1 Muriel S Ouedraogo,2 Serge Theophile Soubeiga,3,4 Theodora Mahoukede Zohoncon,1,5 Paul Ouedraogo,5 Dorcas Obiri-Yeboah,6 Aziz Sidi Aristide Tapsoba,1 Touwendpoulimdé Isabelle Kiendrebeogo,1,3 Tani Sagna,4 Pascal Niamba,2 Adama Traore,2 Jacques Simpore1,3 1Biochemistry and Microbiology Department, Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, Ouagadougou, Burkina Faso; 2Department of Dermatology, Yalgado Ouedraogo Hospital University Centre, Ouagadougou, Burkina Faso; 3Department of Biomedical Research, Biomolecular Research Centre Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso; 4Department of Biomedical and Public Health, Research Institute of Health Sciences (IRSS), Ouagadougou, Burkina Faso; 5Department of Medicine, Saint Thomas d’Aquin University (USTA), Ouagadougou, Burkina Faso; 6Department of Microbiology and Immunology, University of Cape Coast, Cape Coast, GhanaCorrespondence: Serge Theophile Soubeiga, Department of Biomedical and Public Health, Research Institute for Health Sciences (IRSS), P.O. 03. BOX 7047, Ouagadougou, Burkina Faso, Tel +226 70 01 30 58, Email theo.soubeiga@gmail.comIntroduction: Human herpesvirus type 8 (HHV-8) is the main etiological agent of Kaposi’s sarcoma. This virus is frequently associated with immunocompromision. This study aimed to detect HHV-8 in people with compromised immune system.Patients and Methods: This is a cross-sectional study that included 180 subjects: 179 HIV-infected patients and 1 patient with bullous pemphigoid. Blood samples were taken from all subjects, and swabs of lesions were then taken from individuals with symptoms of Kaposi’s sarcoma. Viral load and CD4+ T lymphocytes count were performed for persons living with HIV and real-time PCR detection of HHV-8 DNA was performed in all subjects in the study.Results: Among HIV-infected persons, 13.41% had a viral load of more than 10,000 copies/mL, and 22.91% had a CD4+ T lymphocytes count of fewer than 350 cells/μL. A total of four (three HIV-1 infected patients and one patient with bullous pemphigoid) patients (2.22%) had apparent lesions of Kaposi’s sarcoma. In the plasmas and swabs from associated lesions, HHV-8 DNA was found in only two individuals, with an HHV-8 prevalence of 1.11% (2/180) with 0.55% (1/179) in an HIV-infected patient on antiretroviral therapy.Conclusion: These results exposing low prevalence levels of HHV-8 in HIV-infected patients could be due to the beneficial effect of antiretroviral drugs.Keywords: HIV, HHV-8, immunocompromised, Kaposi’s sarcoma, Burkina Faso |
| first_indexed | 2024-12-11T18:32:26Z |
| format | Article |
| id | doaj.art-300641c151254152bdd8971034546300 |
| institution | Directory Open Access Journal |
| issn | 1179-1373 |
| language | English |
| last_indexed | 2024-12-11T18:32:26Z |
| publishDate | 2022-07-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | HIV/AIDS: Research and Palliative Care |
| spelling | doaj.art-300641c151254152bdd89710345463002022-12-22T00:54:52ZengDove Medical PressHIV/AIDS: Research and Palliative Care1179-13732022-07-01Volume 1431131776445Molecular Epidemiology of Human Herpes Virus Type 8 Among Patients with Compromised Immune System in Ouagadougou, Burkina FasoBiatougou NMBOuedraogo MSSoubeiga STZohoncon TMOuedraogo PObiri-Yeboah DTapsoba ASAKiendrebeogo TISagna TNiamba PTraore ASimpore JNakougou Mo&idigr;-bohm Biatougou,1 Muriel S Ouedraogo,2 Serge Theophile Soubeiga,3,4 Theodora Mahoukede Zohoncon,1,5 Paul Ouedraogo,5 Dorcas Obiri-Yeboah,6 Aziz Sidi Aristide Tapsoba,1 Touwendpoulimdé Isabelle Kiendrebeogo,1,3 Tani Sagna,4 Pascal Niamba,2 Adama Traore,2 Jacques Simpore1,3 1Biochemistry and Microbiology Department, Laboratory of Molecular Biology and Genetics (LABIOGENE), University Joseph Ki-Zerbo, Ouagadougou, Burkina Faso; 2Department of Dermatology, Yalgado Ouedraogo Hospital University Centre, Ouagadougou, Burkina Faso; 3Department of Biomedical Research, Biomolecular Research Centre Pietro Annigoni (CERBA), Ouagadougou, Burkina Faso; 4Department of Biomedical and Public Health, Research Institute of Health Sciences (IRSS), Ouagadougou, Burkina Faso; 5Department of Medicine, Saint Thomas d’Aquin University (USTA), Ouagadougou, Burkina Faso; 6Department of Microbiology and Immunology, University of Cape Coast, Cape Coast, GhanaCorrespondence: Serge Theophile Soubeiga, Department of Biomedical and Public Health, Research Institute for Health Sciences (IRSS), P.O. 03. BOX 7047, Ouagadougou, Burkina Faso, Tel +226 70 01 30 58, Email theo.soubeiga@gmail.comIntroduction: Human herpesvirus type 8 (HHV-8) is the main etiological agent of Kaposi’s sarcoma. This virus is frequently associated with immunocompromision. This study aimed to detect HHV-8 in people with compromised immune system.Patients and Methods: This is a cross-sectional study that included 180 subjects: 179 HIV-infected patients and 1 patient with bullous pemphigoid. Blood samples were taken from all subjects, and swabs of lesions were then taken from individuals with symptoms of Kaposi’s sarcoma. Viral load and CD4+ T lymphocytes count were performed for persons living with HIV and real-time PCR detection of HHV-8 DNA was performed in all subjects in the study.Results: Among HIV-infected persons, 13.41% had a viral load of more than 10,000 copies/mL, and 22.91% had a CD4+ T lymphocytes count of fewer than 350 cells/μL. A total of four (three HIV-1 infected patients and one patient with bullous pemphigoid) patients (2.22%) had apparent lesions of Kaposi’s sarcoma. In the plasmas and swabs from associated lesions, HHV-8 DNA was found in only two individuals, with an HHV-8 prevalence of 1.11% (2/180) with 0.55% (1/179) in an HIV-infected patient on antiretroviral therapy.Conclusion: These results exposing low prevalence levels of HHV-8 in HIV-infected patients could be due to the beneficial effect of antiretroviral drugs.Keywords: HIV, HHV-8, immunocompromised, Kaposi’s sarcoma, Burkina Fasohttps://www.dovepress.com/molecular-epidemiology-of-human-herpes-virus-type-8-among-patients-wit-peer-reviewed-fulltext-article-HIVhivhhv-8immunosuppressionkaposi’s sarcomaburkina faso |
| spellingShingle | Biatougou NMB Ouedraogo MS Soubeiga ST Zohoncon TM Ouedraogo P Obiri-Yeboah D Tapsoba ASA Kiendrebeogo TI Sagna T Niamba P Traore A Simpore J Molecular Epidemiology of Human Herpes Virus Type 8 Among Patients with Compromised Immune System in Ouagadougou, Burkina Faso HIV/AIDS: Research and Palliative Care hiv hhv-8 immunosuppression kaposi’s sarcoma burkina faso |
| title | Molecular Epidemiology of Human Herpes Virus Type 8 Among Patients with Compromised Immune System in Ouagadougou, Burkina Faso |
| title_full | Molecular Epidemiology of Human Herpes Virus Type 8 Among Patients with Compromised Immune System in Ouagadougou, Burkina Faso |
| title_fullStr | Molecular Epidemiology of Human Herpes Virus Type 8 Among Patients with Compromised Immune System in Ouagadougou, Burkina Faso |
| title_full_unstemmed | Molecular Epidemiology of Human Herpes Virus Type 8 Among Patients with Compromised Immune System in Ouagadougou, Burkina Faso |
| title_short | Molecular Epidemiology of Human Herpes Virus Type 8 Among Patients with Compromised Immune System in Ouagadougou, Burkina Faso |
| title_sort | molecular epidemiology of human herpes virus type 8 among patients with compromised immune system in ouagadougou burkina faso |
| topic | hiv hhv-8 immunosuppression kaposi’s sarcoma burkina faso |
| url | https://www.dovepress.com/molecular-epidemiology-of-human-herpes-virus-type-8-among-patients-wit-peer-reviewed-fulltext-article-HIV |
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