<i>In vitro</i> function of random donor platelets stored for 7 days in composol platelet additive solution
<b>Background and Aim:</b> Platelets are routinely isolated from whole blood and stored in plasma for 5 days. The present study was done to assess the <i>in vitro</i> function of random donor platelets stored for 7 days in composol platelet additive solution at 22°C...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2011-01-01
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| Series: | Asian Journal of Transfusion Science |
| Subjects: | |
| Online Access: | http://www.ajts.org/article.asp?issn=0973-6247;year=2011;volume=5;issue=1;spage=11;epage=14;aulast=Gupta |
| _version_ | 1811309167759065088 |
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| author | Gupta Ashish Chandra Tulika Kumar Ashutosh |
| author_facet | Gupta Ashish Chandra Tulika Kumar Ashutosh |
| author_sort | Gupta Ashish |
| collection | DOAJ |
| description | <b>Background and Aim:</b> Platelets are routinely isolated from whole blood and stored in plasma for 5 days. The present study was done to assess the <i>in vitro</i> function of random donor platelets stored for 7 days in composol platelet additive solution at 22°C. <b>Materials and Methods:</b> The study sample included 30 blood donors of both sex in State Blood Bank, CSM Medical University, Lucknow. Random donor platelets were prepared by platelet rich plasma method. Whole blood (350 ml) was collected in anticoagulant Citrate Phosphate Dextrose Adenine triple blood bags. Random donor platelets were stored for 7 days at 22°C in platelet incubators and agitators, with and without additive solution. <b>Results:</b> Platelet swirling was present in all the units at 22°C on day 7, with no evidence of bacterial contamination. Comparison of the mean values of platelet count, platelet factor 3, lactate dehydrogenase, pH, glucose and platelet aggregation showed no significant difference in additive solution, whereas platelet factor 3, glucose and platelet aggregation showed significant difference (<i>P</i> < 0.001) on day 7 without additive solution at 22°C. <b>Conclusion:</b> Our study infers that platelet viability and aggregation were best maintained within normal levels on day 7 of storage in platelet additive solution at 22°C. Thus, we may conclude that <i>in vitro</i> storage of random donor platelets with an extended shelf life of 7 days using platelet additive solution may be advocated to improve the inventory of platelets. |
| first_indexed | 2024-04-13T09:37:33Z |
| format | Article |
| id | doaj.art-300643670ab54e019651b4e6c6d8a5b1 |
| institution | Directory Open Access Journal |
| issn | 0973-6247 1998-3565 |
| language | English |
| last_indexed | 2024-04-13T09:37:33Z |
| publishDate | 2011-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Journal of Transfusion Science |
| spelling | doaj.art-300643670ab54e019651b4e6c6d8a5b12022-12-22T02:52:02ZengWolters Kluwer Medknow PublicationsAsian Journal of Transfusion Science0973-62471998-35652011-01-01511114<i>In vitro</i> function of random donor platelets stored for 7 days in composol platelet additive solutionGupta AshishChandra TulikaKumar Ashutosh<b>Background and Aim:</b> Platelets are routinely isolated from whole blood and stored in plasma for 5 days. The present study was done to assess the <i>in vitro</i> function of random donor platelets stored for 7 days in composol platelet additive solution at 22°C. <b>Materials and Methods:</b> The study sample included 30 blood donors of both sex in State Blood Bank, CSM Medical University, Lucknow. Random donor platelets were prepared by platelet rich plasma method. Whole blood (350 ml) was collected in anticoagulant Citrate Phosphate Dextrose Adenine triple blood bags. Random donor platelets were stored for 7 days at 22°C in platelet incubators and agitators, with and without additive solution. <b>Results:</b> Platelet swirling was present in all the units at 22°C on day 7, with no evidence of bacterial contamination. Comparison of the mean values of platelet count, platelet factor 3, lactate dehydrogenase, pH, glucose and platelet aggregation showed no significant difference in additive solution, whereas platelet factor 3, glucose and platelet aggregation showed significant difference (<i>P</i> < 0.001) on day 7 without additive solution at 22°C. <b>Conclusion:</b> Our study infers that platelet viability and aggregation were best maintained within normal levels on day 7 of storage in platelet additive solution at 22°C. Thus, we may conclude that <i>in vitro</i> storage of random donor platelets with an extended shelf life of 7 days using platelet additive solution may be advocated to improve the inventory of platelets.http://www.ajts.org/article.asp?issn=0973-6247;year=2011;volume=5;issue=1;spage=11;epage=14;aulast=GuptaAdditive solution<i>in vitro</i>random donor plateletsstorage |
| spellingShingle | Gupta Ashish Chandra Tulika Kumar Ashutosh <i>In vitro</i> function of random donor platelets stored for 7 days in composol platelet additive solution Asian Journal of Transfusion Science Additive solution <i>in vitro</i> random donor platelets storage |
| title | <i>In vitro</i> function of random donor platelets stored for 7 days in composol platelet additive solution |
| title_full | <i>In vitro</i> function of random donor platelets stored for 7 days in composol platelet additive solution |
| title_fullStr | <i>In vitro</i> function of random donor platelets stored for 7 days in composol platelet additive solution |
| title_full_unstemmed | <i>In vitro</i> function of random donor platelets stored for 7 days in composol platelet additive solution |
| title_short | <i>In vitro</i> function of random donor platelets stored for 7 days in composol platelet additive solution |
| title_sort | i in vitro i function of random donor platelets stored for 7 days in composol platelet additive solution |
| topic | Additive solution <i>in vitro</i> random donor platelets storage |
| url | http://www.ajts.org/article.asp?issn=0973-6247;year=2011;volume=5;issue=1;spage=11;epage=14;aulast=Gupta |
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