Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity
<p>Abstract</p> <p>Background</p> <p>carbon nanotubes (CNT) can have adverse effects on health. Therefore, minimizing the risk associated with CNT exposure is of crucial importance. The aim of this work was to evaluate if coating multi-walled CNT (MWCNT) with polymers c...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2011-01-01
|
| Series: | Particle and Fibre Toxicology |
| Online Access: | http://www.particleandfibretoxicology.com/content/8/1/3 |
| _version_ | 1819123780025319424 |
|---|---|
| author | Rossi Michel J Boczkowski Jorge Simon-Deckers Angélique Setyan Ari Bussy Cyrill Tabet Lyes Lanone Sophie |
| author_facet | Rossi Michel J Boczkowski Jorge Simon-Deckers Angélique Setyan Ari Bussy Cyrill Tabet Lyes Lanone Sophie |
| author_sort | Rossi Michel J |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>carbon nanotubes (CNT) can have adverse effects on health. Therefore, minimizing the risk associated with CNT exposure is of crucial importance. The aim of this work was to evaluate if coating multi-walled CNT (MWCNT) with polymers could modify their toxicity, thus representing a useful strategy to decrease adverse health effects of CNT. We used industrially-produced MWCNT uncoated (NT1) or coated (50/50 wt%) with acid-based (NT2) or polystyrene-based (NT3) polymer, and exposed murine macrophages (RAW 264.7 cell line) or Balb/c mice by intratracheal administration. Biological experiments were performed both <it>in vitro </it>and <it>in vivo</it>, examining time- and dose-dependent effects of CNT, in terms of cytotoxicity, expression of genes and proteins related to oxidative stress, inflammation and tissue remodeling, cell and lung tissue morphology (optical and transmission electron microscopy), and bronchoalveolar lavage fluid content analysis.</p> <p>Results</p> <p>extensive physico-chemical characterization of MWCNT was performed, and showed, although similar dimensions for the 3 MWCNT, a much smaller specific surface area for NT2 and NT3 as compared to NT1 (54.1, 34 and 227.54 m<sup>2</sup>/g respectively), along with different surface characteristics. MWCNT-induced cytotoxicity, oxidative stress, and inflammation were increased by acid-based and decreased by polystyrene-based polymer coating both <it>in vitro </it>in murine macrophages and <it>in vivo </it>in lung of mice monitored for 6 months.</p> <p>Conclusions</p> <p>these results demonstrate that coating CNT with polymers, without affecting their intrinsic structure, may constitute a useful strategy for decreasing CNT toxicity, and may hold promise for improving occupational safety and that of general the user.</p> |
| first_indexed | 2024-12-22T07:13:47Z |
| format | Article |
| id | doaj.art-300aa59f096b4978b25b2ce7f7e56e80 |
| institution | Directory Open Access Journal |
| issn | 1743-8977 |
| language | English |
| last_indexed | 2024-12-22T07:13:47Z |
| publishDate | 2011-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Particle and Fibre Toxicology |
| spelling | doaj.art-300aa59f096b4978b25b2ce7f7e56e802022-12-21T18:34:27ZengBMCParticle and Fibre Toxicology1743-89772011-01-0181310.1186/1743-8977-8-3Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicityRossi Michel JBoczkowski JorgeSimon-Deckers AngéliqueSetyan AriBussy CyrillTabet LyesLanone Sophie<p>Abstract</p> <p>Background</p> <p>carbon nanotubes (CNT) can have adverse effects on health. Therefore, minimizing the risk associated with CNT exposure is of crucial importance. The aim of this work was to evaluate if coating multi-walled CNT (MWCNT) with polymers could modify their toxicity, thus representing a useful strategy to decrease adverse health effects of CNT. We used industrially-produced MWCNT uncoated (NT1) or coated (50/50 wt%) with acid-based (NT2) or polystyrene-based (NT3) polymer, and exposed murine macrophages (RAW 264.7 cell line) or Balb/c mice by intratracheal administration. Biological experiments were performed both <it>in vitro </it>and <it>in vivo</it>, examining time- and dose-dependent effects of CNT, in terms of cytotoxicity, expression of genes and proteins related to oxidative stress, inflammation and tissue remodeling, cell and lung tissue morphology (optical and transmission electron microscopy), and bronchoalveolar lavage fluid content analysis.</p> <p>Results</p> <p>extensive physico-chemical characterization of MWCNT was performed, and showed, although similar dimensions for the 3 MWCNT, a much smaller specific surface area for NT2 and NT3 as compared to NT1 (54.1, 34 and 227.54 m<sup>2</sup>/g respectively), along with different surface characteristics. MWCNT-induced cytotoxicity, oxidative stress, and inflammation were increased by acid-based and decreased by polystyrene-based polymer coating both <it>in vitro </it>in murine macrophages and <it>in vivo </it>in lung of mice monitored for 6 months.</p> <p>Conclusions</p> <p>these results demonstrate that coating CNT with polymers, without affecting their intrinsic structure, may constitute a useful strategy for decreasing CNT toxicity, and may hold promise for improving occupational safety and that of general the user.</p>http://www.particleandfibretoxicology.com/content/8/1/3 |
| spellingShingle | Rossi Michel J Boczkowski Jorge Simon-Deckers Angélique Setyan Ari Bussy Cyrill Tabet Lyes Lanone Sophie Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity Particle and Fibre Toxicology |
| title | Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity |
| title_full | Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity |
| title_fullStr | Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity |
| title_full_unstemmed | Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity |
| title_short | Coating carbon nanotubes with a polystyrene-based polymer protects against pulmonary toxicity |
| title_sort | coating carbon nanotubes with a polystyrene based polymer protects against pulmonary toxicity |
| url | http://www.particleandfibretoxicology.com/content/8/1/3 |
| work_keys_str_mv | AT rossimichelj coatingcarbonnanotubeswithapolystyrenebasedpolymerprotectsagainstpulmonarytoxicity AT boczkowskijorge coatingcarbonnanotubeswithapolystyrenebasedpolymerprotectsagainstpulmonarytoxicity AT simondeckersangelique coatingcarbonnanotubeswithapolystyrenebasedpolymerprotectsagainstpulmonarytoxicity AT setyanari coatingcarbonnanotubeswithapolystyrenebasedpolymerprotectsagainstpulmonarytoxicity AT bussycyrill coatingcarbonnanotubeswithapolystyrenebasedpolymerprotectsagainstpulmonarytoxicity AT tabetlyes coatingcarbonnanotubeswithapolystyrenebasedpolymerprotectsagainstpulmonarytoxicity AT lanonesophie coatingcarbonnanotubeswithapolystyrenebasedpolymerprotectsagainstpulmonarytoxicity |