Characterization of Plasmodium vivax Early Transcribed Membrane Protein 11.2 and Exported Protein 1.
In Plasmodium, the membrane of intracellular parasites is initially formed during invasion as an invagination of the red blood cell surface, which forms a barrier between the parasite and infected red blood cells in asexual blood stage parasites. The membrane proteins of intracellular parasites of P...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4444142?pdf=render |
| _version_ | 1819051217825824768 |
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| author | Yang Cheng Feng Lu Seong-Kyun Lee Deok-Hoon Kong Kwon-Soo Ha Bo Wang Jetsumon Sattabongkot Takafumi Tsuboi Eun-Taek Han |
| author_facet | Yang Cheng Feng Lu Seong-Kyun Lee Deok-Hoon Kong Kwon-Soo Ha Bo Wang Jetsumon Sattabongkot Takafumi Tsuboi Eun-Taek Han |
| author_sort | Yang Cheng |
| collection | DOAJ |
| description | In Plasmodium, the membrane of intracellular parasites is initially formed during invasion as an invagination of the red blood cell surface, which forms a barrier between the parasite and infected red blood cells in asexual blood stage parasites. The membrane proteins of intracellular parasites of Plasmodium species have been identified such as early-transcribed membrane proteins (ETRAMPs) and exported proteins (EXPs). However, there is little or no information regarding the intracellular parasite membrane in Plasmodium vivax. In the present study, recombinant PvETRAMP11.2 (PVX_003565) and PvEXP1 (PVX_091700) were expressed and evaluated antigenicity tests using sera from P. vivax-infected patients. A large proportion of infected individuals presented with IgG antibody responses against PvETRAMP11.2 (76.8%) and PvEXP1 (69.6%). Both of the recombinant proteins elicited high antibody titers capable of recognizing parasites of vivax malaria patients. PvETRAMP11.2 partially co-localized with PvEXP1 on the intracellular membranes of immature schizont. Moreover, they were also detected at the apical organelles of newly formed merozoites of mature schizont. We first proposed that these proteins might be synthesized in the preceding schizont stage, localized on the parasite membranes and apical organelles of infected erythrocytes, and induced high IgG antibody responses in patients. |
| first_indexed | 2024-12-21T12:00:26Z |
| format | Article |
| id | doaj.art-300c50f956a84a03ba95fc6bdd58e20a |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T12:00:26Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-300c50f956a84a03ba95fc6bdd58e20a2022-12-21T19:04:50ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01105e012750010.1371/journal.pone.0127500Characterization of Plasmodium vivax Early Transcribed Membrane Protein 11.2 and Exported Protein 1.Yang ChengFeng LuSeong-Kyun LeeDeok-Hoon KongKwon-Soo HaBo WangJetsumon SattabongkotTakafumi TsuboiEun-Taek HanIn Plasmodium, the membrane of intracellular parasites is initially formed during invasion as an invagination of the red blood cell surface, which forms a barrier between the parasite and infected red blood cells in asexual blood stage parasites. The membrane proteins of intracellular parasites of Plasmodium species have been identified such as early-transcribed membrane proteins (ETRAMPs) and exported proteins (EXPs). However, there is little or no information regarding the intracellular parasite membrane in Plasmodium vivax. In the present study, recombinant PvETRAMP11.2 (PVX_003565) and PvEXP1 (PVX_091700) were expressed and evaluated antigenicity tests using sera from P. vivax-infected patients. A large proportion of infected individuals presented with IgG antibody responses against PvETRAMP11.2 (76.8%) and PvEXP1 (69.6%). Both of the recombinant proteins elicited high antibody titers capable of recognizing parasites of vivax malaria patients. PvETRAMP11.2 partially co-localized with PvEXP1 on the intracellular membranes of immature schizont. Moreover, they were also detected at the apical organelles of newly formed merozoites of mature schizont. We first proposed that these proteins might be synthesized in the preceding schizont stage, localized on the parasite membranes and apical organelles of infected erythrocytes, and induced high IgG antibody responses in patients.http://europepmc.org/articles/PMC4444142?pdf=render |
| spellingShingle | Yang Cheng Feng Lu Seong-Kyun Lee Deok-Hoon Kong Kwon-Soo Ha Bo Wang Jetsumon Sattabongkot Takafumi Tsuboi Eun-Taek Han Characterization of Plasmodium vivax Early Transcribed Membrane Protein 11.2 and Exported Protein 1. PLoS ONE |
| title | Characterization of Plasmodium vivax Early Transcribed Membrane Protein 11.2 and Exported Protein 1. |
| title_full | Characterization of Plasmodium vivax Early Transcribed Membrane Protein 11.2 and Exported Protein 1. |
| title_fullStr | Characterization of Plasmodium vivax Early Transcribed Membrane Protein 11.2 and Exported Protein 1. |
| title_full_unstemmed | Characterization of Plasmodium vivax Early Transcribed Membrane Protein 11.2 and Exported Protein 1. |
| title_short | Characterization of Plasmodium vivax Early Transcribed Membrane Protein 11.2 and Exported Protein 1. |
| title_sort | characterization of plasmodium vivax early transcribed membrane protein 11 2 and exported protein 1 |
| url | http://europepmc.org/articles/PMC4444142?pdf=render |
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