The Impact of Pre-transplant Cell-free DNA Levels on Leukemia Relapse and Transplant-related Complications in Allogeneic Hematopoietic Stem Cell Transplant Recipients
Background: Cell-free DNA, which may be considered as “liquid” biopsy, may serve as a diagnostic and prognostic marker not only in hematological malignancies but in solid tumors as well. Aims: To investigate the prognostic role of pre-transplant cell-free DNA levels in allogeneic hematopoietic stem...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2020-05-01
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| Series: | Balkan Medical Journal |
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| Online Access: | http://balkanmedicaljournal.org/text.php?lang=en&id=2180 |
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| author | Zeynep Arzu Yegin Ferda Can Sanem Gökçen Rezzan Eren Sadioğlu Zübeyde Nur Özkurt Çiğdem İlhan Münci Yağcı |
| author_facet | Zeynep Arzu Yegin Ferda Can Sanem Gökçen Rezzan Eren Sadioğlu Zübeyde Nur Özkurt Çiğdem İlhan Münci Yağcı |
| author_sort | Zeynep Arzu Yegin |
| collection | DOAJ |
| description | Background: Cell-free DNA, which may be considered as “liquid” biopsy, may serve as a diagnostic and prognostic marker not only in hematological malignancies but in solid tumors as well.
Aims: To investigate the prognostic role of pre-transplant cell-free DNA levels in allogeneic hematopoietic stem cell transplant recipients.
Study Design: Retrospective cohort study.
Methods: A total of 177 allogeneic hematopoietic stem cell transplant recipients [median age: 36 (16-66) years; male/female: 111/66] with an initial diagnosis of acute leukemia were included in the study. Cell-free DNA was extracted from pre-transplant serum samples by using the MagNA Pure Compact Nucleic Acid Isolation Kit I with the MagNA Pure Compact instrument (Roche Diagnostics, Penzberg, Germany).
Results: A positive correlation was demonstrated between cell-free DNA and age (p=0.018; r=0.177). Pre-transplant cell-free DNA levels were lower in bcr-abl (+) patients (p=0.001), while an adverse correlation was indicated between cell-free DNA and bcr-abl levels (p=0.001; r=−0.531). Acute lymphoblastic leukemia patients with bcr-abl positivity (p=0.001) or abnormal cytogenetics (p=0.038) represented significantly lower pre-transplant cell-free DNA levels. Cell-free DNA levels were lower in patients who developed sinusoidal obstruction syndrome (p=0.035). In terms of long-term complications, acute myeloid leukemia patients who experienced post-transplant relapse had significantly lower pre-transplant cell-free DNA levels (p=0.024). Overall survival was not statistically different between high- and low- cell-free DNA groups (45.2% vs 22.5; p=0.821).
Conclusion: In general, low serum levels of pre-transplant çell-free DNA seem to be associated with transplant-related morbidities and may be considered an adverse prognostic factor for allogeneic hematopoietic stem cell transplant recipients. |
| first_indexed | 2024-04-10T11:01:54Z |
| format | Article |
| id | doaj.art-300f6ad16e9f4944b05aeb4600e3403c |
| institution | Directory Open Access Journal |
| issn | 2146-3123 2146-3131 |
| language | English |
| last_indexed | 2024-04-10T11:01:54Z |
| publishDate | 2020-05-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Balkan Medical Journal |
| spelling | doaj.art-300f6ad16e9f4944b05aeb4600e3403c2023-02-15T16:19:38ZengGalenos Publishing HouseBalkan Medical Journal2146-31232146-31312020-05-0137313814310.4274/balkanmedj.galenos.2020.2019.8.25The Impact of Pre-transplant Cell-free DNA Levels on Leukemia Relapse and Transplant-related Complications in Allogeneic Hematopoietic Stem Cell Transplant RecipientsZeynep Arzu Yegin0https://orcid.org/0000-0002-0212-9663Ferda Can1https://orcid.org/0000-0002-9899-1441Sanem Gökçen2https://orcid.org/0000-0003-3436-5086Rezzan Eren Sadioğlu3https://orcid.org/0000-0001-9761-0320Zübeyde Nur Özkurt4https://orcid.org/0000-0001-9834-6058Çiğdem İlhan5https://orcid.org/0000-0002-9196-0116Münci Yağcı6https://orcid.org/0000-0002-0458-2920Department of Hematology, Gazi University School of Medicine, Ankara, TurkeyDepartment of Hematology, Gazi University School of Medicine, Ankara, TurkeyDepartment of Hematology, Gazi University School of Medicine, Ankara, TurkeyDepartment of Internal Medicine, Gazi University School of Medicine, Ankara, TurkeyDepartment of Hematology, Gazi University School of Medicine, Ankara, TurkeyDepartment of Hematology, Gazi University School of Medicine, Ankara, TurkeyDepartment of Hematology, Gazi University School of Medicine, Ankara, TurkeyBackground: Cell-free DNA, which may be considered as “liquid” biopsy, may serve as a diagnostic and prognostic marker not only in hematological malignancies but in solid tumors as well. Aims: To investigate the prognostic role of pre-transplant cell-free DNA levels in allogeneic hematopoietic stem cell transplant recipients. Study Design: Retrospective cohort study. Methods: A total of 177 allogeneic hematopoietic stem cell transplant recipients [median age: 36 (16-66) years; male/female: 111/66] with an initial diagnosis of acute leukemia were included in the study. Cell-free DNA was extracted from pre-transplant serum samples by using the MagNA Pure Compact Nucleic Acid Isolation Kit I with the MagNA Pure Compact instrument (Roche Diagnostics, Penzberg, Germany). Results: A positive correlation was demonstrated between cell-free DNA and age (p=0.018; r=0.177). Pre-transplant cell-free DNA levels were lower in bcr-abl (+) patients (p=0.001), while an adverse correlation was indicated between cell-free DNA and bcr-abl levels (p=0.001; r=−0.531). Acute lymphoblastic leukemia patients with bcr-abl positivity (p=0.001) or abnormal cytogenetics (p=0.038) represented significantly lower pre-transplant cell-free DNA levels. Cell-free DNA levels were lower in patients who developed sinusoidal obstruction syndrome (p=0.035). In terms of long-term complications, acute myeloid leukemia patients who experienced post-transplant relapse had significantly lower pre-transplant cell-free DNA levels (p=0.024). Overall survival was not statistically different between high- and low- cell-free DNA groups (45.2% vs 22.5; p=0.821). Conclusion: In general, low serum levels of pre-transplant çell-free DNA seem to be associated with transplant-related morbidities and may be considered an adverse prognostic factor for allogeneic hematopoietic stem cell transplant recipients.http://balkanmedicaljournal.org/text.php?lang=en&id=2180acute lymphoblastic leukemiaacute myeloid leukemiaallogeneic hematopoietic stem cell transplantationcell-free dna |
| spellingShingle | Zeynep Arzu Yegin Ferda Can Sanem Gökçen Rezzan Eren Sadioğlu Zübeyde Nur Özkurt Çiğdem İlhan Münci Yağcı The Impact of Pre-transplant Cell-free DNA Levels on Leukemia Relapse and Transplant-related Complications in Allogeneic Hematopoietic Stem Cell Transplant Recipients Balkan Medical Journal acute lymphoblastic leukemia acute myeloid leukemia allogeneic hematopoietic stem cell transplantation cell-free dna |
| title | The Impact of Pre-transplant Cell-free DNA Levels on Leukemia Relapse and Transplant-related Complications in Allogeneic Hematopoietic Stem Cell Transplant Recipients |
| title_full | The Impact of Pre-transplant Cell-free DNA Levels on Leukemia Relapse and Transplant-related Complications in Allogeneic Hematopoietic Stem Cell Transplant Recipients |
| title_fullStr | The Impact of Pre-transplant Cell-free DNA Levels on Leukemia Relapse and Transplant-related Complications in Allogeneic Hematopoietic Stem Cell Transplant Recipients |
| title_full_unstemmed | The Impact of Pre-transplant Cell-free DNA Levels on Leukemia Relapse and Transplant-related Complications in Allogeneic Hematopoietic Stem Cell Transplant Recipients |
| title_short | The Impact of Pre-transplant Cell-free DNA Levels on Leukemia Relapse and Transplant-related Complications in Allogeneic Hematopoietic Stem Cell Transplant Recipients |
| title_sort | impact of pre transplant cell free dna levels on leukemia relapse and transplant related complications in allogeneic hematopoietic stem cell transplant recipients |
| topic | acute lymphoblastic leukemia acute myeloid leukemia allogeneic hematopoietic stem cell transplantation cell-free dna |
| url | http://balkanmedicaljournal.org/text.php?lang=en&id=2180 |
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