Lemon extract supported green synthesis of bimetallic CuO/Ag nanoporous materials for sensitive detection of vitamin D3
Abstract In modern era, deficiency of Vitamin D3 is predominantly due to limited exposure to sunlight and UV radiation resulting from indoor lifestyles. Several studies have revealed that vitamin D deficiency can lead to chronic vascular inflammation, diabetes mellitus, hypertension, congestive left...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-46774-w |
| _version_ | 1797388394650664960 |
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| author | Gowhar A. Naikoo Fay M. Almashali Fatima A. S. Habis Mustri Bano Jahangir Ahmad Rather Israr U. Hassan Rayees Ahmad Sheikh Palanisamy Kannan Iman M. Alfagih Murtaza M. Tambuwala |
| author_facet | Gowhar A. Naikoo Fay M. Almashali Fatima A. S. Habis Mustri Bano Jahangir Ahmad Rather Israr U. Hassan Rayees Ahmad Sheikh Palanisamy Kannan Iman M. Alfagih Murtaza M. Tambuwala |
| author_sort | Gowhar A. Naikoo |
| collection | DOAJ |
| description | Abstract In modern era, deficiency of Vitamin D3 is predominantly due to limited exposure to sunlight and UV radiation resulting from indoor lifestyles. Several studies have revealed that vitamin D deficiency can lead to chronic vascular inflammation, diabetes mellitus, hypertension, congestive left ventricular hypertrophy, and heart failure. This study introduces a green synthesis of novel bimetallic nanoporous composite, CuO/Ag using lemon extract. The synthesized nanoporous material, CuO/Ag@lemon extract was characterized using several analytical techniques, including X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). The CuO/Ag@lemon extract nanoparticles were immobilized on glassy carbon electrode (GCE) to prepare modified CuO/Ag@lemon extract–GCE interface. The electrocatalytic and electrochemical properties investigation was carried out on the modified electrode. using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and amperometry for detecting of Vitamin D3. The DPV method displayed a linear response range of 0.02–22.5 µM with a detection limit of 2.62 nM, while the amperometric method showed a broader linear range of 0.25–23.25 µM with a detection limit of 2.70 nM with 82% modified electrode stability. The designed electrode exhibited a positive response to the inclusion of Vitamin D3 with electro-oxidation, reaching steady-state within 3.4 s, with 87% reproducibility within a day. The proposed method offers a rapid and sensitive platform for detection of Vitamin D3 with minimal interference from other molecules. The early diagnosis of Vitamin D3 deficiency using modified electrodes allows for early treatment, thereby preventing severe health complications. |
| first_indexed | 2024-03-08T22:40:11Z |
| format | Article |
| id | doaj.art-3010866174f347d68b6dbbaa9431975a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-08T22:40:11Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-3010866174f347d68b6dbbaa9431975a2023-12-17T12:13:27ZengNature PortfolioScientific Reports2045-23222023-11-0113111210.1038/s41598-023-46774-wLemon extract supported green synthesis of bimetallic CuO/Ag nanoporous materials for sensitive detection of vitamin D3Gowhar A. Naikoo0Fay M. Almashali1Fatima A. S. Habis2Mustri Bano3Jahangir Ahmad Rather4Israr U. Hassan5Rayees Ahmad Sheikh6Palanisamy Kannan7Iman M. Alfagih8Murtaza M. Tambuwala9Department of Mathematics and Sciences, College of Arts and Applied Sciences, Dhofar UniversityDepartment of Chemical Engineering, College of Engineering, Dhofar UniversityDepartment of Chemical Engineering, College of Engineering, Dhofar UniversityDepartment of Mathematics and Sciences, College of Arts and Applied Sciences, Dhofar UniversityDepartment of Chemistry, Govt. Degree College PulwamaDepartment of Mathematics and Sciences, College of Arts and Applied Sciences, Dhofar UniversityDepartment of Chemistry, Govt. Degree College PulwamaCollege of Biological, Chemical Sciences and Engineering, Jiaxing UniversityDepartment of Pharmaceutics, College of Pharmacy, King Saud UniversityLincoln Medical School - Universities of Nottingham and Lincoln, University of LincolnAbstract In modern era, deficiency of Vitamin D3 is predominantly due to limited exposure to sunlight and UV radiation resulting from indoor lifestyles. Several studies have revealed that vitamin D deficiency can lead to chronic vascular inflammation, diabetes mellitus, hypertension, congestive left ventricular hypertrophy, and heart failure. This study introduces a green synthesis of novel bimetallic nanoporous composite, CuO/Ag using lemon extract. The synthesized nanoporous material, CuO/Ag@lemon extract was characterized using several analytical techniques, including X-ray diffraction (XRD), transmission electron microscopy (TEM), scanning electron microscopy (SEM), and energy-dispersive X-ray spectroscopy (EDX). The CuO/Ag@lemon extract nanoparticles were immobilized on glassy carbon electrode (GCE) to prepare modified CuO/Ag@lemon extract–GCE interface. The electrocatalytic and electrochemical properties investigation was carried out on the modified electrode. using cyclic voltammetry (CV), differential pulse voltammetry (DPV), and amperometry for detecting of Vitamin D3. The DPV method displayed a linear response range of 0.02–22.5 µM with a detection limit of 2.62 nM, while the amperometric method showed a broader linear range of 0.25–23.25 µM with a detection limit of 2.70 nM with 82% modified electrode stability. The designed electrode exhibited a positive response to the inclusion of Vitamin D3 with electro-oxidation, reaching steady-state within 3.4 s, with 87% reproducibility within a day. The proposed method offers a rapid and sensitive platform for detection of Vitamin D3 with minimal interference from other molecules. The early diagnosis of Vitamin D3 deficiency using modified electrodes allows for early treatment, thereby preventing severe health complications.https://doi.org/10.1038/s41598-023-46774-w |
| spellingShingle | Gowhar A. Naikoo Fay M. Almashali Fatima A. S. Habis Mustri Bano Jahangir Ahmad Rather Israr U. Hassan Rayees Ahmad Sheikh Palanisamy Kannan Iman M. Alfagih Murtaza M. Tambuwala Lemon extract supported green synthesis of bimetallic CuO/Ag nanoporous materials for sensitive detection of vitamin D3 Scientific Reports |
| title | Lemon extract supported green synthesis of bimetallic CuO/Ag nanoporous materials for sensitive detection of vitamin D3 |
| title_full | Lemon extract supported green synthesis of bimetallic CuO/Ag nanoporous materials for sensitive detection of vitamin D3 |
| title_fullStr | Lemon extract supported green synthesis of bimetallic CuO/Ag nanoporous materials for sensitive detection of vitamin D3 |
| title_full_unstemmed | Lemon extract supported green synthesis of bimetallic CuO/Ag nanoporous materials for sensitive detection of vitamin D3 |
| title_short | Lemon extract supported green synthesis of bimetallic CuO/Ag nanoporous materials for sensitive detection of vitamin D3 |
| title_sort | lemon extract supported green synthesis of bimetallic cuo ag nanoporous materials for sensitive detection of vitamin d3 |
| url | https://doi.org/10.1038/s41598-023-46774-w |
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