Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation
Objective The aim of our study was to evaluate the effect of two different doses of lycopene, an antioxidant, on experimentally induced ovarian ischemia/reperfusion (IR) injury in rat model. Materials and Methods Twenty-four female rats were randomly divided into four groups: sham operation (group...
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| Format: | Article |
| Language: | English |
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Thieme Medical and Scientific Publishers Pvt. Ltd.
2020-03-01
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| Series: | Journal of Laboratory Physicians |
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| Online Access: | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0040-1715553 |
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| author | Emsal Pinar Topdagi Yilmaz Harun Un Betul Gundogdu Elif Polat Seda Askin Yunus Emre Topdagi Zekai Halici |
| author_facet | Emsal Pinar Topdagi Yilmaz Harun Un Betul Gundogdu Elif Polat Seda Askin Yunus Emre Topdagi Zekai Halici |
| author_sort | Emsal Pinar Topdagi Yilmaz |
| collection | DOAJ |
| description | Objective The aim of our study was to evaluate the effect of two different doses of lycopene, an antioxidant, on experimentally induced ovarian ischemia/reperfusion (IR) injury in rat model.
Materials and Methods Twenty-four female rats were randomly divided into four groups: sham operation (group 1), 3-hour ischemia, 3-hour reperfusion (IR) (group 2), and IR + 100 mg/kg lycopene (PO) (group 3), IR + 200 mg/kg of lycopene (group 4). The rats’ superoxide dismutase (SOD), myeloperoxidase (MPO) activities, malondialdehyde (MDA), and glutathione (GSH) levels were calculated. Ovarian tissue damage was assessed using a histopathological scoring system.
Results Serum parameter levels and histological scores showed that treatment with lycopene may be conservative approach to prevent IR injury after the ovarian detorsion procedure.The improvement with lycopene was higher at 200 mg than at 100 mg. The MPO and MDA values were significantly lower in groups 3 and 4 as compared with group 2 (p < 0.05), whereas the MPO and MDA values were lower in group 4 as compared with group 3.The SOD and GSH values were significantly higher in groups 3 and 4 as compared with group 2 (p < 0.05), whereas the SOD and GSH values were higher in group 4 as compared with group 3.Tissue damage scores were elevated in the IR group compared with the sham group, but the treatment with different lycopene doses after reperfusion improved the histopathological tissue damage scores.
Conclusion The results showed that lycopene treatment reduced ovarian IR damage. Antioxidant activity was found to increase in a dose-dependent manner. Lycopene treatment may be conservative approach for ovarian torsion patients after the detorsion procedure to prevent IR damage. |
| first_indexed | 2024-12-23T05:12:25Z |
| format | Article |
| id | doaj.art-3011e6e8283746df96ac42d78602cdf6 |
| institution | Directory Open Access Journal |
| issn | 0974-2727 0974-7826 |
| language | English |
| last_indexed | 2024-12-23T05:12:25Z |
| publishDate | 2020-03-01 |
| publisher | Thieme Medical and Scientific Publishers Pvt. Ltd. |
| record_format | Article |
| series | Journal of Laboratory Physicians |
| spelling | doaj.art-3011e6e8283746df96ac42d78602cdf62022-12-21T17:58:55ZengThieme Medical and Scientific Publishers Pvt. Ltd.Journal of Laboratory Physicians0974-27270974-78262020-03-011201323710.1055/s-0040-1715553Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological EvaluationEmsal Pinar Topdagi Yilmaz0Harun Un1Betul Gundogdu2Elif Polat3Seda Askin4Yunus Emre Topdagi5Zekai Halici6Department of Gynecology and Obstetrics, Faculty of Medicine, Ataturk University, Erzurum, TurkeyDepartment of Biochemistry, Faculty of Pharmacy, Ağrı Ibrahim Cecen University, Agri, TurkeyDepartment of Pathology, Faculty of Medicine, Ataturk University, Erzurum, TurkeyDepartment of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, TurkeyDepartment of Biochemistry, Faculty of Medicine, Ataturk University, Erzurum, TurkeyDepartment of Gynecology and Obstetrics, Faculty of Medicine, Sanko University, Gaziantep, TurkeyDepartment of Pharmacology, Faculty of Medicine, Ataturk University, Erzurum, TurkeyObjective The aim of our study was to evaluate the effect of two different doses of lycopene, an antioxidant, on experimentally induced ovarian ischemia/reperfusion (IR) injury in rat model. Materials and Methods Twenty-four female rats were randomly divided into four groups: sham operation (group 1), 3-hour ischemia, 3-hour reperfusion (IR) (group 2), and IR + 100 mg/kg lycopene (PO) (group 3), IR + 200 mg/kg of lycopene (group 4). The rats’ superoxide dismutase (SOD), myeloperoxidase (MPO) activities, malondialdehyde (MDA), and glutathione (GSH) levels were calculated. Ovarian tissue damage was assessed using a histopathological scoring system. Results Serum parameter levels and histological scores showed that treatment with lycopene may be conservative approach to prevent IR injury after the ovarian detorsion procedure.The improvement with lycopene was higher at 200 mg than at 100 mg. The MPO and MDA values were significantly lower in groups 3 and 4 as compared with group 2 (p < 0.05), whereas the MPO and MDA values were lower in group 4 as compared with group 3.The SOD and GSH values were significantly higher in groups 3 and 4 as compared with group 2 (p < 0.05), whereas the SOD and GSH values were higher in group 4 as compared with group 3.Tissue damage scores were elevated in the IR group compared with the sham group, but the treatment with different lycopene doses after reperfusion improved the histopathological tissue damage scores. Conclusion The results showed that lycopene treatment reduced ovarian IR damage. Antioxidant activity was found to increase in a dose-dependent manner. Lycopene treatment may be conservative approach for ovarian torsion patients after the detorsion procedure to prevent IR damage.http://www.thieme-connect.de/DOI/DOI?10.1055/s-0040-1715553ischemia/reperfusionlycopeneovarian torsionoxidative stress |
| spellingShingle | Emsal Pinar Topdagi Yilmaz Harun Un Betul Gundogdu Elif Polat Seda Askin Yunus Emre Topdagi Zekai Halici Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation Journal of Laboratory Physicians ischemia/reperfusion lycopene ovarian torsion oxidative stress |
| title | Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation |
| title_full | Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation |
| title_fullStr | Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation |
| title_full_unstemmed | Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation |
| title_short | Protective Effect of Lycopene against Reperfusion Injury in Rats with Ovarian Torsion: A Biochemical and Histopathological Evaluation |
| title_sort | protective effect of lycopene against reperfusion injury in rats with ovarian torsion a biochemical and histopathological evaluation |
| topic | ischemia/reperfusion lycopene ovarian torsion oxidative stress |
| url | http://www.thieme-connect.de/DOI/DOI?10.1055/s-0040-1715553 |
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