The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae <i>Isochrysis galbana</i> Based on the Transcriptome Analysis
The adverse effects of microplastics on microalgae species have been extensively studied, but their impact on the bait microalgae entering the food chain has not been well understood. This study investigated the cytological and physiological response of <i>Isochrysis galbana</i> to polye...
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| Format: | Article |
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/5/1108 |
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| author | Xinfeng Xiao Wenfang Li Shuangwei Li Xingsheng Zuo Jie Liu Linke Guo Xiao Lu Linlin Zhang |
| author_facet | Xinfeng Xiao Wenfang Li Shuangwei Li Xingsheng Zuo Jie Liu Linke Guo Xiao Lu Linlin Zhang |
| author_sort | Xinfeng Xiao |
| collection | DOAJ |
| description | The adverse effects of microplastics on microalgae species have been extensively studied, but their impact on the bait microalgae entering the food chain has not been well understood. This study investigated the cytological and physiological response of <i>Isochrysis galbana</i> to polyethylene microplastics (PE-MPs, 10 μm) and nanoplastics (PE-NPs, 50 nm). The results showed that PE-MPs had no significant impact on <i>I. galbana</i>, while PsE-NPs obviously inhibited cell growth, reduced chlorophyll content, and caused a decline in carotenoids and soluble protein. These changes in the quality of <i>I. galbana</i> could negatively affect its use as aquaculture feed. To understand the molecular response mechanism of <i>I. galbana</i> to PE-NPs, transcriptome sequencing was performed. The result revealed that the TCA cycle, purine metabolism, and some key amino acid syntheses were down-regulated by PE-NPs, while the Calvin cycle and fatty acid metabolism were up-regulated to tolerate PE-NP pressure. Microbial analysis showed that the bacterial community structure associated with <i>I. galbana</i> was significantly altered at the species level by PE-NPs. In conclusion, this study provides new insights into the physiological stress response caused by microplastic pollution based on transcriptome and bacterial community analysis. The findings highlight the need to mitigate the release of microplastics into the environment to prevent their harmful effects on aquatic ecosystems and will be helpful in understanding the impact of polyethylene nanoplastics on the bait microalgae. |
| first_indexed | 2024-03-11T03:29:05Z |
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| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T03:29:05Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-3014275822c04624a280fa479a444c312023-11-18T02:31:56ZengMDPI AGMicroorganisms2076-26072023-04-01115110810.3390/microorganisms11051108The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae <i>Isochrysis galbana</i> Based on the Transcriptome AnalysisXinfeng Xiao0Wenfang Li1Shuangwei Li2Xingsheng Zuo3Jie Liu4Linke Guo5Xiao Lu6Linlin Zhang7College of Safety & Environmental Engineering, Shandong University of Science & Technology, Qingdao 266510, ChinaCollege of Safety & Environmental Engineering, Shandong University of Science & Technology, Qingdao 266510, ChinaCollege of Safety & Environmental Engineering, Shandong University of Science & Technology, Qingdao 266510, ChinaCollege of Safety & Environmental Engineering, Shandong University of Science & Technology, Qingdao 266510, ChinaCollege of Safety & Environmental Engineering, Shandong University of Science & Technology, Qingdao 266510, ChinaCollege of Safety & Environmental Engineering, Shandong University of Science & Technology, Qingdao 266510, ChinaCollege of Safety & Environmental Engineering, Shandong University of Science & Technology, Qingdao 266510, ChinaCollege of Safety & Environmental Engineering, Shandong University of Science & Technology, Qingdao 266510, ChinaThe adverse effects of microplastics on microalgae species have been extensively studied, but their impact on the bait microalgae entering the food chain has not been well understood. This study investigated the cytological and physiological response of <i>Isochrysis galbana</i> to polyethylene microplastics (PE-MPs, 10 μm) and nanoplastics (PE-NPs, 50 nm). The results showed that PE-MPs had no significant impact on <i>I. galbana</i>, while PsE-NPs obviously inhibited cell growth, reduced chlorophyll content, and caused a decline in carotenoids and soluble protein. These changes in the quality of <i>I. galbana</i> could negatively affect its use as aquaculture feed. To understand the molecular response mechanism of <i>I. galbana</i> to PE-NPs, transcriptome sequencing was performed. The result revealed that the TCA cycle, purine metabolism, and some key amino acid syntheses were down-regulated by PE-NPs, while the Calvin cycle and fatty acid metabolism were up-regulated to tolerate PE-NP pressure. Microbial analysis showed that the bacterial community structure associated with <i>I. galbana</i> was significantly altered at the species level by PE-NPs. In conclusion, this study provides new insights into the physiological stress response caused by microplastic pollution based on transcriptome and bacterial community analysis. The findings highlight the need to mitigate the release of microplastics into the environment to prevent their harmful effects on aquatic ecosystems and will be helpful in understanding the impact of polyethylene nanoplastics on the bait microalgae.https://www.mdpi.com/2076-2607/11/5/1108microplastics<i>Isochrysis galbana</i>transcriptome analysisbacterial community |
| spellingShingle | Xinfeng Xiao Wenfang Li Shuangwei Li Xingsheng Zuo Jie Liu Linke Guo Xiao Lu Linlin Zhang The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae <i>Isochrysis galbana</i> Based on the Transcriptome Analysis Microorganisms microplastics <i>Isochrysis galbana</i> transcriptome analysis bacterial community |
| title | The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae <i>Isochrysis galbana</i> Based on the Transcriptome Analysis |
| title_full | The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae <i>Isochrysis galbana</i> Based on the Transcriptome Analysis |
| title_fullStr | The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae <i>Isochrysis galbana</i> Based on the Transcriptome Analysis |
| title_full_unstemmed | The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae <i>Isochrysis galbana</i> Based on the Transcriptome Analysis |
| title_short | The Growth Inhibition of Polyethylene Nanoplastics on the Bait-Microalgae <i>Isochrysis galbana</i> Based on the Transcriptome Analysis |
| title_sort | growth inhibition of polyethylene nanoplastics on the bait microalgae i isochrysis galbana i based on the transcriptome analysis |
| topic | microplastics <i>Isochrysis galbana</i> transcriptome analysis bacterial community |
| url | https://www.mdpi.com/2076-2607/11/5/1108 |
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