Defensin alpha 6 (<it>DEFA 6</it>) overexpression threshold of over 60 fold can distinguish between adenoma and fully blown colon carcinoma in individual patients
<p>Abstract</p> <p>Background</p> <p>It is known that alpha-defensin expression is enhanced in colon cancer. However, the expression of human alpha defensin 6 (DEFA 6) in earlier stages, such as adenoma, has so far not yet been studied in a patient resolved manner.</...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2010-10-01
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| Series: | BMC Cancer |
| Online Access: | http://www.biomedcentral.com/1471-2407/10/588 |
| _version_ | 1818150141762207744 |
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| author | Greulich Karl Settmacher Utz Glei Michael Wilhelm Anne Jahns Franziska Radeva Mariya Y Mothes Henning |
| author_facet | Greulich Karl Settmacher Utz Glei Michael Wilhelm Anne Jahns Franziska Radeva Mariya Y Mothes Henning |
| author_sort | Greulich Karl |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>It is known that alpha-defensin expression is enhanced in colon cancer. However, the expression of human alpha defensin 6 (DEFA 6) in earlier stages, such as adenoma, has so far not yet been studied in a patient resolved manner.</p> <p>Methods</p> <p>By using quantitative Real Time-PCR, the gene expression pattern of <it>DEFA 1-3 </it>and <it>DEFA 6 </it>was analyzed in tissue of different stages of carcinogenesis, derived from colorectal cancer patients. In addition to paired normal and tumor tissue, matched normal near tumor and adenoma tissue samples were examined.</p> <p>Results</p> <p>The median gene expression of human defensin alpha 6 (<it>DEFA 6</it>) has been found to be moderately increased (~ 5 fold) in tumor samples derived from individuals with colorectal cancer (CRC) when compared to their normal counterparts. However, when the data were analyzed in a patient-wise manner, a large expression variation among individual patients is found, making the use of <it>DEFA 6 </it>for individual diagnosis of fully blown colon carcinoma difficult. Surprisingly, in adenoma the gene expression analysis revealed a 100 fold increased median expression of <it>DEFA 6 </it>relative to normal colon tissue. 13/18 samples had an individual overexpression of more than 60 fold in adenoma but only 3/17 in carcinoma. In each of the individual patients, at least either the adenoma or the carcinoma showed strong <it>DEFA 6 </it>overexpression.</p> <p>Conclusions</p> <p>We suggest that the expression of <it>DEFA 6 </it>preferably can be used as a potential diagnostic marker for adenoma and not as a marker for fully blown carcinoma. This is supported by the fact that <it>DEFA 6 </it>is a downstream target of the Wnt pathway, which is mutational active during the earliest stage of cancer development.</p> |
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| format | Article |
| id | doaj.art-301645d79c0148748d043514cbabb65a |
| institution | Directory Open Access Journal |
| issn | 1471-2407 |
| language | English |
| last_indexed | 2024-12-11T13:18:13Z |
| publishDate | 2010-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj.art-301645d79c0148748d043514cbabb65a2022-12-22T01:05:58ZengBMCBMC Cancer1471-24072010-10-0110158810.1186/1471-2407-10-588Defensin alpha 6 (<it>DEFA 6</it>) overexpression threshold of over 60 fold can distinguish between adenoma and fully blown colon carcinoma in individual patientsGreulich KarlSettmacher UtzGlei MichaelWilhelm AnneJahns FranziskaRadeva Mariya YMothes Henning<p>Abstract</p> <p>Background</p> <p>It is known that alpha-defensin expression is enhanced in colon cancer. However, the expression of human alpha defensin 6 (DEFA 6) in earlier stages, such as adenoma, has so far not yet been studied in a patient resolved manner.</p> <p>Methods</p> <p>By using quantitative Real Time-PCR, the gene expression pattern of <it>DEFA 1-3 </it>and <it>DEFA 6 </it>was analyzed in tissue of different stages of carcinogenesis, derived from colorectal cancer patients. In addition to paired normal and tumor tissue, matched normal near tumor and adenoma tissue samples were examined.</p> <p>Results</p> <p>The median gene expression of human defensin alpha 6 (<it>DEFA 6</it>) has been found to be moderately increased (~ 5 fold) in tumor samples derived from individuals with colorectal cancer (CRC) when compared to their normal counterparts. However, when the data were analyzed in a patient-wise manner, a large expression variation among individual patients is found, making the use of <it>DEFA 6 </it>for individual diagnosis of fully blown colon carcinoma difficult. Surprisingly, in adenoma the gene expression analysis revealed a 100 fold increased median expression of <it>DEFA 6 </it>relative to normal colon tissue. 13/18 samples had an individual overexpression of more than 60 fold in adenoma but only 3/17 in carcinoma. In each of the individual patients, at least either the adenoma or the carcinoma showed strong <it>DEFA 6 </it>overexpression.</p> <p>Conclusions</p> <p>We suggest that the expression of <it>DEFA 6 </it>preferably can be used as a potential diagnostic marker for adenoma and not as a marker for fully blown carcinoma. This is supported by the fact that <it>DEFA 6 </it>is a downstream target of the Wnt pathway, which is mutational active during the earliest stage of cancer development.</p>http://www.biomedcentral.com/1471-2407/10/588 |
| spellingShingle | Greulich Karl Settmacher Utz Glei Michael Wilhelm Anne Jahns Franziska Radeva Mariya Y Mothes Henning Defensin alpha 6 (<it>DEFA 6</it>) overexpression threshold of over 60 fold can distinguish between adenoma and fully blown colon carcinoma in individual patients BMC Cancer |
| title | Defensin alpha 6 (<it>DEFA 6</it>) overexpression threshold of over 60 fold can distinguish between adenoma and fully blown colon carcinoma in individual patients |
| title_full | Defensin alpha 6 (<it>DEFA 6</it>) overexpression threshold of over 60 fold can distinguish between adenoma and fully blown colon carcinoma in individual patients |
| title_fullStr | Defensin alpha 6 (<it>DEFA 6</it>) overexpression threshold of over 60 fold can distinguish between adenoma and fully blown colon carcinoma in individual patients |
| title_full_unstemmed | Defensin alpha 6 (<it>DEFA 6</it>) overexpression threshold of over 60 fold can distinguish between adenoma and fully blown colon carcinoma in individual patients |
| title_short | Defensin alpha 6 (<it>DEFA 6</it>) overexpression threshold of over 60 fold can distinguish between adenoma and fully blown colon carcinoma in individual patients |
| title_sort | defensin alpha 6 it defa 6 it overexpression threshold of over 60 fold can distinguish between adenoma and fully blown colon carcinoma in individual patients |
| url | http://www.biomedcentral.com/1471-2407/10/588 |
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