Detection of human cytomegalovirus in glioblastoma among Taiwanese subjects.
The relationship between human cytomegalovirus (HCMV) and glioblastoma (GBM) has been debated for more than a decade. We investigated the presence of HCMV genes, RNA and protein in GBMs and their relationships with tumor progression. Results of quantitative PCR for HCMV UL73, nested PCR for HCMV UL1...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC5464665?pdf=render |
| _version_ | 1819131199758532608 |
|---|---|
| author | Ching-Fen Yang Hsiang-Ling Ho Shih-Chieh Lin Chih-Yi Hsu Donald Ming-Tak Ho |
| author_facet | Ching-Fen Yang Hsiang-Ling Ho Shih-Chieh Lin Chih-Yi Hsu Donald Ming-Tak Ho |
| author_sort | Ching-Fen Yang |
| collection | DOAJ |
| description | The relationship between human cytomegalovirus (HCMV) and glioblastoma (GBM) has been debated for more than a decade. We investigated the presence of HCMV genes, RNA and protein in GBMs and their relationships with tumor progression. Results of quantitative PCR for HCMV UL73, nested PCR for HCMV UL144, in situ hybridization (ISH) for RNA transcript, and immunohistochemistry (IHC) for protein expression and their relationship to the prognosis of 116 patients with GBM were evaluated. Nine (7.8%) cases revealed a low concentration of HCMV UL73, and only 2 of the 9 (1.7%) cases showed consistent positivity on repeat PCR testing. HCMV UL144, ISH and IHC assays were all negative. The HCMV UL73 positive cases did not show significant difference in the clinicopathological characters including age, gender, Karnofsky performance status, extent of resection, bevacizumab treatment, isocitrate dehydrogenase 1 mutation, O6-methylguanine-DNA-methyltranferase status and Ki67 labeling index, and did not reveal prognostic significance. As only one HCMV gene was detected at low concentration in 7.8% of GBMs and there was no evidence of transcription, protein expression or prognostic impact, we cannot conclude a relationship between HCMV and GBM in Taiwanese patients. |
| first_indexed | 2024-12-22T09:11:43Z |
| format | Article |
| id | doaj.art-301789c84ae944cf8a2d43b700da25c6 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T09:11:43Z |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-301789c84ae944cf8a2d43b700da25c62022-12-21T18:31:24ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01126e017936610.1371/journal.pone.0179366Detection of human cytomegalovirus in glioblastoma among Taiwanese subjects.Ching-Fen YangHsiang-Ling HoShih-Chieh LinChih-Yi HsuDonald Ming-Tak HoThe relationship between human cytomegalovirus (HCMV) and glioblastoma (GBM) has been debated for more than a decade. We investigated the presence of HCMV genes, RNA and protein in GBMs and their relationships with tumor progression. Results of quantitative PCR for HCMV UL73, nested PCR for HCMV UL144, in situ hybridization (ISH) for RNA transcript, and immunohistochemistry (IHC) for protein expression and their relationship to the prognosis of 116 patients with GBM were evaluated. Nine (7.8%) cases revealed a low concentration of HCMV UL73, and only 2 of the 9 (1.7%) cases showed consistent positivity on repeat PCR testing. HCMV UL144, ISH and IHC assays were all negative. The HCMV UL73 positive cases did not show significant difference in the clinicopathological characters including age, gender, Karnofsky performance status, extent of resection, bevacizumab treatment, isocitrate dehydrogenase 1 mutation, O6-methylguanine-DNA-methyltranferase status and Ki67 labeling index, and did not reveal prognostic significance. As only one HCMV gene was detected at low concentration in 7.8% of GBMs and there was no evidence of transcription, protein expression or prognostic impact, we cannot conclude a relationship between HCMV and GBM in Taiwanese patients.http://europepmc.org/articles/PMC5464665?pdf=render |
| spellingShingle | Ching-Fen Yang Hsiang-Ling Ho Shih-Chieh Lin Chih-Yi Hsu Donald Ming-Tak Ho Detection of human cytomegalovirus in glioblastoma among Taiwanese subjects. PLoS ONE |
| title | Detection of human cytomegalovirus in glioblastoma among Taiwanese subjects. |
| title_full | Detection of human cytomegalovirus in glioblastoma among Taiwanese subjects. |
| title_fullStr | Detection of human cytomegalovirus in glioblastoma among Taiwanese subjects. |
| title_full_unstemmed | Detection of human cytomegalovirus in glioblastoma among Taiwanese subjects. |
| title_short | Detection of human cytomegalovirus in glioblastoma among Taiwanese subjects. |
| title_sort | detection of human cytomegalovirus in glioblastoma among taiwanese subjects |
| url | http://europepmc.org/articles/PMC5464665?pdf=render |
| work_keys_str_mv | AT chingfenyang detectionofhumancytomegalovirusinglioblastomaamongtaiwanesesubjects AT hsianglingho detectionofhumancytomegalovirusinglioblastomaamongtaiwanesesubjects AT shihchiehlin detectionofhumancytomegalovirusinglioblastomaamongtaiwanesesubjects AT chihyihsu detectionofhumancytomegalovirusinglioblastomaamongtaiwanesesubjects AT donaldmingtakho detectionofhumancytomegalovirusinglioblastomaamongtaiwanesesubjects |