Initial characterization of gap phase introduction in every cell cycle of C. elegans embryogenesis
Early embryonic cell cycles usually alternate between S and M phases without any gap phase. When the gap phases are developmentally introduced in various cell types remains poorly defined especially during embryogenesis. To establish the cell-specific introduction of gap phases in embryo, we generat...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-10-01
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| Series: | Frontiers in Cell and Developmental Biology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcell.2022.978962/full |
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| author | Ming-Kin Wong Vincy Wing Sze Ho Xiaotai Huang Xiaotai Huang Lu-Yan Chan Dongying Xie Runsheng Li Xiaoliang Ren Guoye Guan Yiming Ma Boyi Hu Boyi Hu Hong Yan Zhongying Zhao Zhongying Zhao |
| author_facet | Ming-Kin Wong Vincy Wing Sze Ho Xiaotai Huang Xiaotai Huang Lu-Yan Chan Dongying Xie Runsheng Li Xiaoliang Ren Guoye Guan Yiming Ma Boyi Hu Boyi Hu Hong Yan Zhongying Zhao Zhongying Zhao |
| author_sort | Ming-Kin Wong |
| collection | DOAJ |
| description | Early embryonic cell cycles usually alternate between S and M phases without any gap phase. When the gap phases are developmentally introduced in various cell types remains poorly defined especially during embryogenesis. To establish the cell-specific introduction of gap phases in embryo, we generate multiple fluorescence ubiquitin cell cycle indicators (FUCCI) in C. elegans. Time-lapse 3D imaging followed by lineal expression profiling reveals sharp and differential accumulation of the FUCCI reporters, allowing the systematic demarcation of cell cycle phases throughout embryogenesis. Accumulation of the reporters reliably identifies both G1 and G2 phases only in two embryonic cells with an extended cell cycle length, suggesting that the remaining cells divide either without a G1 phase, or with a brief G1 phase that is too short to be picked up by our reporters. In summary, we provide an initial picture of gap phase introduction in a metazoan embryo. The newly developed FUCCI reporters pave the way for further characterization of developmental control of cell cycle progression. |
| first_indexed | 2024-04-11T19:42:32Z |
| format | Article |
| id | doaj.art-30186eecdbbb4769b2c972fae928d9f6 |
| institution | Directory Open Access Journal |
| issn | 2296-634X |
| language | English |
| last_indexed | 2024-04-11T19:42:32Z |
| publishDate | 2022-10-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cell and Developmental Biology |
| spelling | doaj.art-30186eecdbbb4769b2c972fae928d9f62022-12-22T04:06:40ZengFrontiers Media S.A.Frontiers in Cell and Developmental Biology2296-634X2022-10-011010.3389/fcell.2022.978962978962Initial characterization of gap phase introduction in every cell cycle of C. elegans embryogenesisMing-Kin Wong0Vincy Wing Sze Ho1Xiaotai Huang2Xiaotai Huang3Lu-Yan Chan4Dongying Xie5Runsheng Li6Xiaoliang Ren7Guoye Guan8Yiming Ma9Boyi Hu10Boyi Hu11Hong Yan12Zhongying Zhao13Zhongying Zhao14Department of Biology, Hong Kong Baptist University, Kowloon, Hong Kong SAR, ChinaDepartment of Biology, Hong Kong Baptist University, Kowloon, Hong Kong SAR, ChinaSchool of Computer Science and Technology, Xidian University, Xi’an, ChinaDepartment of Electronic Engineering, City University of Hong Kong, Kowloon, Hong Kong SAR, ChinaDepartment of Biology, Hong Kong Baptist University, Kowloon, Hong Kong SAR, ChinaDepartment of Biology, Hong Kong Baptist University, Kowloon, Hong Kong SAR, ChinaDepartment of Biology, Hong Kong Baptist University, Kowloon, Hong Kong SAR, ChinaDepartment of Biology, Hong Kong Baptist University, Kowloon, Hong Kong SAR, ChinaCenter for Quantitative Biology, Peking University, Beijing, ChinaDepartment of Biology, Hong Kong Baptist University, Kowloon, Hong Kong SAR, ChinaDepartment of Biology, Hong Kong Baptist University, Kowloon, Hong Kong SAR, ChinaDepartment of Biology, Southern University of Science and Technology, Shenzhen, ChinaDepartment of Electronic Engineering, City University of Hong Kong, Kowloon, Hong Kong SAR, ChinaDepartment of Biology, Hong Kong Baptist University, Kowloon, Hong Kong SAR, ChinaState Key Laboratory of Environmental and Biological Analysis, Hong Kong Baptist University, Kowloon, Hong Kong SAR, ChinaEarly embryonic cell cycles usually alternate between S and M phases without any gap phase. When the gap phases are developmentally introduced in various cell types remains poorly defined especially during embryogenesis. To establish the cell-specific introduction of gap phases in embryo, we generate multiple fluorescence ubiquitin cell cycle indicators (FUCCI) in C. elegans. Time-lapse 3D imaging followed by lineal expression profiling reveals sharp and differential accumulation of the FUCCI reporters, allowing the systematic demarcation of cell cycle phases throughout embryogenesis. Accumulation of the reporters reliably identifies both G1 and G2 phases only in two embryonic cells with an extended cell cycle length, suggesting that the remaining cells divide either without a G1 phase, or with a brief G1 phase that is too short to be picked up by our reporters. In summary, we provide an initial picture of gap phase introduction in a metazoan embryo. The newly developed FUCCI reporters pave the way for further characterization of developmental control of cell cycle progression.https://www.frontiersin.org/articles/10.3389/fcell.2022.978962/fullFUCCIcell cycle phaseembryogenesisC. eleganscell lineage |
| spellingShingle | Ming-Kin Wong Vincy Wing Sze Ho Xiaotai Huang Xiaotai Huang Lu-Yan Chan Dongying Xie Runsheng Li Xiaoliang Ren Guoye Guan Yiming Ma Boyi Hu Boyi Hu Hong Yan Zhongying Zhao Zhongying Zhao Initial characterization of gap phase introduction in every cell cycle of C. elegans embryogenesis Frontiers in Cell and Developmental Biology FUCCI cell cycle phase embryogenesis C. elegans cell lineage |
| title | Initial characterization of gap phase introduction in every cell cycle of C. elegans embryogenesis |
| title_full | Initial characterization of gap phase introduction in every cell cycle of C. elegans embryogenesis |
| title_fullStr | Initial characterization of gap phase introduction in every cell cycle of C. elegans embryogenesis |
| title_full_unstemmed | Initial characterization of gap phase introduction in every cell cycle of C. elegans embryogenesis |
| title_short | Initial characterization of gap phase introduction in every cell cycle of C. elegans embryogenesis |
| title_sort | initial characterization of gap phase introduction in every cell cycle of c elegans embryogenesis |
| topic | FUCCI cell cycle phase embryogenesis C. elegans cell lineage |
| url | https://www.frontiersin.org/articles/10.3389/fcell.2022.978962/full |
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