CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo
Caspase-3 carries out the executioner phase of apoptosis, however under special circumstances, cells can survive its activity. To document systematically where and when cells survive caspase-3 activation in vivo, we designed a system, CasExpress, which drives fluorescent protein expression, transien...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2016-04-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/10936 |
| _version_ | 1818018497474592768 |
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| author | Austin Xun Ding Gongping Sun Yewubdar G Argaw Jessica O Wong Sreesankar Easwaran Denise J Montell |
| author_facet | Austin Xun Ding Gongping Sun Yewubdar G Argaw Jessica O Wong Sreesankar Easwaran Denise J Montell |
| author_sort | Austin Xun Ding |
| collection | DOAJ |
| description | Caspase-3 carries out the executioner phase of apoptosis, however under special circumstances, cells can survive its activity. To document systematically where and when cells survive caspase-3 activation in vivo, we designed a system, CasExpress, which drives fluorescent protein expression, transiently or permanently, in cells that survive caspase-3 activation in Drosophila. We discovered widespread survival of caspase-3 activity. Distinct spatial and temporal patterns emerged in different tissues. Some cells activated caspase-3 during their normal development in every cell and in every animal without evidence of apoptosis. In other tissues, such as the brain, expression was sporadic both temporally and spatially and overlapped with periods of apoptosis. In adults, reporter expression was evident in a large fraction of cells in most tissues of every animal; however the precise patterns varied. Inhibition of caspase activity in wing discs reduced wing size demonstrating functional significance. The implications of these patterns are discussed. |
| first_indexed | 2024-04-14T07:40:16Z |
| format | Article |
| id | doaj.art-3018d108051d4b92baf551baa5ef86e4 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-14T07:40:16Z |
| publishDate | 2016-04-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-3018d108051d4b92baf551baa5ef86e42022-12-22T02:05:30ZengeLife Sciences Publications LtdeLife2050-084X2016-04-01510.7554/eLife.10936CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivoAustin Xun Ding0Gongping Sun1Yewubdar G Argaw2Jessica O Wong3Sreesankar Easwaran4Denise J Montell5https://orcid.org/0000-0001-8924-5925Molecular, Cellular and Developmental Biology Department, University of California, Santa Barbara, Santa Barbara, United States; Department of Biological Chemistry, Center for Cell Dynamics, Johns Hopkins School of Medicine, Baltimore, United StatesMolecular, Cellular and Developmental Biology Department, University of California, Santa Barbara, Santa Barbara, United StatesMolecular, Cellular and Developmental Biology Department, University of California, Santa Barbara, Santa Barbara, United StatesMolecular, Cellular and Developmental Biology Department, University of California, Santa Barbara, Santa Barbara, United StatesMolecular, Cellular and Developmental Biology Department, University of California, Santa Barbara, Santa Barbara, United StatesMolecular, Cellular and Developmental Biology Department, University of California, Santa Barbara, Santa Barbara, United States; Department of Biological Chemistry, Center for Cell Dynamics, Johns Hopkins School of Medicine, Baltimore, United StatesCaspase-3 carries out the executioner phase of apoptosis, however under special circumstances, cells can survive its activity. To document systematically where and when cells survive caspase-3 activation in vivo, we designed a system, CasExpress, which drives fluorescent protein expression, transiently or permanently, in cells that survive caspase-3 activation in Drosophila. We discovered widespread survival of caspase-3 activity. Distinct spatial and temporal patterns emerged in different tissues. Some cells activated caspase-3 during their normal development in every cell and in every animal without evidence of apoptosis. In other tissues, such as the brain, expression was sporadic both temporally and spatially and overlapped with periods of apoptosis. In adults, reporter expression was evident in a large fraction of cells in most tissues of every animal; however the precise patterns varied. Inhibition of caspase activity in wing discs reduced wing size demonstrating functional significance. The implications of these patterns are discussed.https://elifesciences.org/articles/10936caspasecell survivalgenetically encoded reporteranastasiscell competitionapoptosis |
| spellingShingle | Austin Xun Ding Gongping Sun Yewubdar G Argaw Jessica O Wong Sreesankar Easwaran Denise J Montell CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo eLife caspase cell survival genetically encoded reporter anastasis cell competition apoptosis |
| title | CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo |
| title_full | CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo |
| title_fullStr | CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo |
| title_full_unstemmed | CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo |
| title_short | CasExpress reveals widespread and diverse patterns of cell survival of caspase-3 activation during development in vivo |
| title_sort | casexpress reveals widespread and diverse patterns of cell survival of caspase 3 activation during development in vivo |
| topic | caspase cell survival genetically encoded reporter anastasis cell competition apoptosis |
| url | https://elifesciences.org/articles/10936 |
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