Genetic characteristics and pathogenesis of H5 low pathogenic avian influenza viruses from wild birds and domestic ducks in South Korea
Abstract H5 and H7 subtypes of low pathogenic avian influenza viruses (LPAIVs) can mutate to highly pathogenic forms and are therefore subject to stringent controls. We characterized H5 LPAIVs isolated from wild-bird habitats and duck farms in South Korea from 2010 to 2017. Through nationwide active...
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| Format: | Article |
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Nature Portfolio
2020-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-020-68720-w |
| _version_ | 1818991667951173632 |
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| author | Yu-Na Lee Dong-Hun Lee Sun-Ha Cheon Yu-Ri Park Yoon-Gi Baek Young-Jae Si Soo-Jeong Kye Eun-Kyoung Lee Gyeong-Beom Heo You-Chan Bae Myoung-Heon Lee Youn-Jeong Lee |
| author_facet | Yu-Na Lee Dong-Hun Lee Sun-Ha Cheon Yu-Ri Park Yoon-Gi Baek Young-Jae Si Soo-Jeong Kye Eun-Kyoung Lee Gyeong-Beom Heo You-Chan Bae Myoung-Heon Lee Youn-Jeong Lee |
| author_sort | Yu-Na Lee |
| collection | DOAJ |
| description | Abstract H5 and H7 subtypes of low pathogenic avian influenza viruses (LPAIVs) can mutate to highly pathogenic forms and are therefore subject to stringent controls. We characterized H5 LPAIVs isolated from wild-bird habitats and duck farms in South Korea from 2010 to 2017. Through nationwide active surveillance for AIVs, 59 H5 LPAIVs were isolated from wild-bird habitats (a mean annual rate of 5.3% of AIV isolations). In 2015, one LPAI H5N3 strain was isolated on a duck farm. Phylogenetic analysis revealed that the hemagglutinin (HA) gene of H5 isolates belonged to the Eurasian lineage, classified into three subgroups (HA-II, HA-III, and HA-IV). The H5 LPAIVs of the HA-III and HA-IV subgroups appeared in 2015 and 2017 in unusually high proportions (13.1% and 14.4%, respectively). In gene-constellation analysis, H5 LPAIVs isolated from 2015 to 2017 constituted ≥ 35 distinct genotypes, representing high levels of genetic diversity. Representative strains of three HA subgroups replicated restrictively in specific-pathogen-free chickens. Among the 11 isolates that were tested, 10 infected and replicated in mice without prior adaptation. The frequency of recent H5 LPAIV isolates with high genetic diversity indicates the importance of continued surveillance in both wild birds and poultry to monitor genetic and pathobiological changes. |
| first_indexed | 2024-12-20T20:13:55Z |
| format | Article |
| id | doaj.art-301b6ed4367b4920981257f159115e35 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T20:13:55Z |
| publishDate | 2020-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-301b6ed4367b4920981257f159115e352022-12-21T19:27:45ZengNature PortfolioScientific Reports2045-23222020-07-0110111110.1038/s41598-020-68720-wGenetic characteristics and pathogenesis of H5 low pathogenic avian influenza viruses from wild birds and domestic ducks in South KoreaYu-Na Lee0Dong-Hun Lee1Sun-Ha Cheon2Yu-Ri Park3Yoon-Gi Baek4Young-Jae Si5Soo-Jeong Kye6Eun-Kyoung Lee7Gyeong-Beom Heo8You-Chan Bae9Myoung-Heon Lee10Youn-Jeong Lee11Avian Influenza Research and Diagnostic Division, Animal and Plant Quarantine AgencyDepartment of Pathobiology and Veterinary Science, University of ConnecticutAvian Influenza Research and Diagnostic Division, Animal and Plant Quarantine AgencyAvian Influenza Research and Diagnostic Division, Animal and Plant Quarantine AgencyAvian Influenza Research and Diagnostic Division, Animal and Plant Quarantine AgencyAvian Influenza Research and Diagnostic Division, Animal and Plant Quarantine AgencyAvian Influenza Research and Diagnostic Division, Animal and Plant Quarantine AgencyAvian Influenza Research and Diagnostic Division, Animal and Plant Quarantine AgencyAvian Influenza Research and Diagnostic Division, Animal and Plant Quarantine AgencyAvian Disease Division, Animal and Plant Quarantine AgencyAvian Influenza Research and Diagnostic Division, Animal and Plant Quarantine AgencyAvian Influenza Research and Diagnostic Division, Animal and Plant Quarantine AgencyAbstract H5 and H7 subtypes of low pathogenic avian influenza viruses (LPAIVs) can mutate to highly pathogenic forms and are therefore subject to stringent controls. We characterized H5 LPAIVs isolated from wild-bird habitats and duck farms in South Korea from 2010 to 2017. Through nationwide active surveillance for AIVs, 59 H5 LPAIVs were isolated from wild-bird habitats (a mean annual rate of 5.3% of AIV isolations). In 2015, one LPAI H5N3 strain was isolated on a duck farm. Phylogenetic analysis revealed that the hemagglutinin (HA) gene of H5 isolates belonged to the Eurasian lineage, classified into three subgroups (HA-II, HA-III, and HA-IV). The H5 LPAIVs of the HA-III and HA-IV subgroups appeared in 2015 and 2017 in unusually high proportions (13.1% and 14.4%, respectively). In gene-constellation analysis, H5 LPAIVs isolated from 2015 to 2017 constituted ≥ 35 distinct genotypes, representing high levels of genetic diversity. Representative strains of three HA subgroups replicated restrictively in specific-pathogen-free chickens. Among the 11 isolates that were tested, 10 infected and replicated in mice without prior adaptation. The frequency of recent H5 LPAIV isolates with high genetic diversity indicates the importance of continued surveillance in both wild birds and poultry to monitor genetic and pathobiological changes.https://doi.org/10.1038/s41598-020-68720-w |
| spellingShingle | Yu-Na Lee Dong-Hun Lee Sun-Ha Cheon Yu-Ri Park Yoon-Gi Baek Young-Jae Si Soo-Jeong Kye Eun-Kyoung Lee Gyeong-Beom Heo You-Chan Bae Myoung-Heon Lee Youn-Jeong Lee Genetic characteristics and pathogenesis of H5 low pathogenic avian influenza viruses from wild birds and domestic ducks in South Korea Scientific Reports |
| title | Genetic characteristics and pathogenesis of H5 low pathogenic avian influenza viruses from wild birds and domestic ducks in South Korea |
| title_full | Genetic characteristics and pathogenesis of H5 low pathogenic avian influenza viruses from wild birds and domestic ducks in South Korea |
| title_fullStr | Genetic characteristics and pathogenesis of H5 low pathogenic avian influenza viruses from wild birds and domestic ducks in South Korea |
| title_full_unstemmed | Genetic characteristics and pathogenesis of H5 low pathogenic avian influenza viruses from wild birds and domestic ducks in South Korea |
| title_short | Genetic characteristics and pathogenesis of H5 low pathogenic avian influenza viruses from wild birds and domestic ducks in South Korea |
| title_sort | genetic characteristics and pathogenesis of h5 low pathogenic avian influenza viruses from wild birds and domestic ducks in south korea |
| url | https://doi.org/10.1038/s41598-020-68720-w |
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