Isolation and analyses of lamina propria lymphocytes from mouse intestines
Summary: Investigating intestinal immune responses is critical to understanding local and systemic immunity. However, obtaining resident intestinal immune cells with high cell viability can be challenging. Here, we provide an optimized protocol to isolate lamina propria lymphocytes from the small an...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | STAR Protocols |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722002465 |
| _version_ | 1818200256451444736 |
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| author | Eunha Kim Melissa Tran Yanyi Sun Jun R. Huh |
| author_facet | Eunha Kim Melissa Tran Yanyi Sun Jun R. Huh |
| author_sort | Eunha Kim |
| collection | DOAJ |
| description | Summary: Investigating intestinal immune responses is critical to understanding local and systemic immunity. However, obtaining resident intestinal immune cells with high cell viability can be challenging. Here, we provide an optimized protocol to isolate lamina propria lymphocytes from the small and large intestines, including lymphocyte activation for cytokine expression analysis and techniques for surface and intracellular antibody staining and flow cytometry. This protocol can be used for isolating and analyzing tissue-resident immune cells from other tissues with specified modifications.For complete details on the use and execution of this protocol, please refer to Kim et al. (2022). |
| first_indexed | 2024-12-12T02:34:46Z |
| format | Article |
| id | doaj.art-301f3250b3124b62904e0246591a43b7 |
| institution | Directory Open Access Journal |
| issn | 2666-1667 |
| language | English |
| last_indexed | 2024-12-12T02:34:46Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj.art-301f3250b3124b62904e0246591a43b72022-12-22T00:41:19ZengElsevierSTAR Protocols2666-16672022-06-0132101366Isolation and analyses of lamina propria lymphocytes from mouse intestinesEunha Kim0Melissa Tran1Yanyi Sun2Jun R. Huh3Department of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; Corresponding authorDepartment of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USADepartment of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USADepartment of Immunology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA; Evergrande Center for Immunologic Diseases, Harvard Medical School and Brigham and Women’s Hospital, Boston, MA 02115, USA; Corresponding authorSummary: Investigating intestinal immune responses is critical to understanding local and systemic immunity. However, obtaining resident intestinal immune cells with high cell viability can be challenging. Here, we provide an optimized protocol to isolate lamina propria lymphocytes from the small and large intestines, including lymphocyte activation for cytokine expression analysis and techniques for surface and intracellular antibody staining and flow cytometry. This protocol can be used for isolating and analyzing tissue-resident immune cells from other tissues with specified modifications.For complete details on the use and execution of this protocol, please refer to Kim et al. (2022).http://www.sciencedirect.com/science/article/pii/S2666166722002465Cell isolationSingle CellFlow Cytometry/Mass CytometryImmunology |
| spellingShingle | Eunha Kim Melissa Tran Yanyi Sun Jun R. Huh Isolation and analyses of lamina propria lymphocytes from mouse intestines STAR Protocols Cell isolation Single Cell Flow Cytometry/Mass Cytometry Immunology |
| title | Isolation and analyses of lamina propria lymphocytes from mouse intestines |
| title_full | Isolation and analyses of lamina propria lymphocytes from mouse intestines |
| title_fullStr | Isolation and analyses of lamina propria lymphocytes from mouse intestines |
| title_full_unstemmed | Isolation and analyses of lamina propria lymphocytes from mouse intestines |
| title_short | Isolation and analyses of lamina propria lymphocytes from mouse intestines |
| title_sort | isolation and analyses of lamina propria lymphocytes from mouse intestines |
| topic | Cell isolation Single Cell Flow Cytometry/Mass Cytometry Immunology |
| url | http://www.sciencedirect.com/science/article/pii/S2666166722002465 |
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