Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteria
Abstract Background Medicinal plants have been founded as traditional herbal medicine worldwide. Most of the plant’s therapeutic properties are due to the presence of secondary metabolites such as alkaloids, glycosides, tannins and volatile oil. Methods The present investigation analyzed the High-Pr...
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| Format: | Article |
| Language: | English |
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BMC
2018-05-01
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| Series: | BMC Complementary and Alternative Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12906-018-2207-8 |
| _version_ | 1819098273219084288 |
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| author | Hazir Rahman Ilyas Khan Anwar Hussain Abdelaaty Abdelaziz Shahat Abdul Tawab Muhammad Qasim Muhammad Adnan Mansour S. Al-Said Riaz Ullah Shahid Niaz Khan |
| author_facet | Hazir Rahman Ilyas Khan Anwar Hussain Abdelaaty Abdelaziz Shahat Abdul Tawab Muhammad Qasim Muhammad Adnan Mansour S. Al-Said Riaz Ullah Shahid Niaz Khan |
| author_sort | Hazir Rahman |
| collection | DOAJ |
| description | Abstract Background Medicinal plants have been founded as traditional herbal medicine worldwide. Most of the plant’s therapeutic properties are due to the presence of secondary metabolites such as alkaloids, glycosides, tannins and volatile oil. Methods The present investigation analyzed the High-Pressure Liquid Chromatography (HPLC) fractions of Glycyrrhiza glabra (Aqueous, Chloroform, Ethanol and Hexane) against multidrug resistant human bacterial pathogens (Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus and Pseudomonas aeruginosa). All the fractions showed antibacterial activity, were subjected to LC MS/MS analysis for identification of bioactive compounds. Results Among total HPLC fractions of G. glabra (n = 20), three HPLC fractions showed potential activity against multidrug resistant (MDR) bacterial isolates. Fraction 1 (F1) of aqueous extracts, showed activity against A. baumannii (15 ± 0.5 mm). F4 from hexane extract of G. glabra showed activity against S. aureus (10 ± 0.2 mm). However, F2 from ethanol extract exhibited activity against S. aureus (10 ± 0.3 mm). These active fractions were further processed by LC MS/MS analysis for the identification of compounds. Ellagic acid was identified in the F1 of aqueous extract while 6-aldehydo-isoophiopogonone was present in F4 of hexane extract. Similarly, Liquirtigenin was identified in F2 of ethanol. Conclusions Glycyrrhiza glabra extracts HPLC fractions showed anti-MDR activity. Three bioactive compounds were identified in the study. 6-aldehydo-isoophiopogonone and Liquirtigenin were for the first time reported in G. glabra. Further characterization of the identified compounds will be helpful for possible therapeutic uses against infectious diseases caused by multidrug resistant bacteria. |
| first_indexed | 2024-12-22T00:28:22Z |
| format | Article |
| id | doaj.art-30214f20b301419094d6206946b2df5e |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-22T00:28:22Z |
| publishDate | 2018-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-30214f20b301419094d6206946b2df5e2022-12-21T18:45:00ZengBMCBMC Complementary and Alternative Medicine1472-68822018-05-011811610.1186/s12906-018-2207-8Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteriaHazir Rahman0Ilyas Khan1Anwar Hussain2Abdelaaty Abdelaziz Shahat3Abdul Tawab4Muhammad Qasim5Muhammad Adnan6Mansour S. Al-Said7Riaz Ullah8Shahid Niaz Khan9Department of Microbiology, Abdul Wali Khan University MardanDepartment of Microbiology, Kohat University of Science & TechnologyDepartment of Botany, Abdul Wali Khan UniversityDepartment of Pharmacognosy and Medicinal Aromatic, and Poisonous Plants Research Center, College of Pharmacy King Saud UniversityNational Institute for Biotechnology and Genetic EngineeringDepartment of Microbiology, Kohat University of Science & TechnologyDepartment of Botany, Kohat University of Science & TechnologyDepartment of Pharmacognosy and Medicinal Aromatic, and Poisonous Plants Research Center, College of Pharmacy King Saud UniversityDepartment of Pharmacognosy and Medicinal Aromatic, and Poisonous Plants Research Center, College of Pharmacy King Saud UniversityDepartment of Zoology, Kohat University of Science & TechnologyAbstract Background Medicinal plants have been founded as traditional herbal medicine worldwide. Most of the plant’s therapeutic properties are due to the presence of secondary metabolites such as alkaloids, glycosides, tannins and volatile oil. Methods The present investigation analyzed the High-Pressure Liquid Chromatography (HPLC) fractions of Glycyrrhiza glabra (Aqueous, Chloroform, Ethanol and Hexane) against multidrug resistant human bacterial pathogens (Escherichia coli, Acinetobacter baumannii, Staphylococcus aureus and Pseudomonas aeruginosa). All the fractions showed antibacterial activity, were subjected to LC MS/MS analysis for identification of bioactive compounds. Results Among total HPLC fractions of G. glabra (n = 20), three HPLC fractions showed potential activity against multidrug resistant (MDR) bacterial isolates. Fraction 1 (F1) of aqueous extracts, showed activity against A. baumannii (15 ± 0.5 mm). F4 from hexane extract of G. glabra showed activity against S. aureus (10 ± 0.2 mm). However, F2 from ethanol extract exhibited activity against S. aureus (10 ± 0.3 mm). These active fractions were further processed by LC MS/MS analysis for the identification of compounds. Ellagic acid was identified in the F1 of aqueous extract while 6-aldehydo-isoophiopogonone was present in F4 of hexane extract. Similarly, Liquirtigenin was identified in F2 of ethanol. Conclusions Glycyrrhiza glabra extracts HPLC fractions showed anti-MDR activity. Three bioactive compounds were identified in the study. 6-aldehydo-isoophiopogonone and Liquirtigenin were for the first time reported in G. glabra. Further characterization of the identified compounds will be helpful for possible therapeutic uses against infectious diseases caused by multidrug resistant bacteria.http://link.springer.com/article/10.1186/s12906-018-2207-8Glycyrrhiza glabraHPLC fractionationAnti-MDR activityEllagic acid 6-aldehydo-isoophiopogononeLiquirtigenin |
| spellingShingle | Hazir Rahman Ilyas Khan Anwar Hussain Abdelaaty Abdelaziz Shahat Abdul Tawab Muhammad Qasim Muhammad Adnan Mansour S. Al-Said Riaz Ullah Shahid Niaz Khan Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteria BMC Complementary and Alternative Medicine Glycyrrhiza glabra HPLC fractionation Anti-MDR activity Ellagic acid 6-aldehydo-isoophiopogonone Liquirtigenin |
| title | Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteria |
| title_full | Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteria |
| title_fullStr | Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteria |
| title_full_unstemmed | Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteria |
| title_short | Glycyrrhiza glabra HPLC fractions: identification of Aldehydo Isoophiopogonone and Liquirtigenin having activity against multidrug resistant bacteria |
| title_sort | glycyrrhiza glabra hplc fractions identification of aldehydo isoophiopogonone and liquirtigenin having activity against multidrug resistant bacteria |
| topic | Glycyrrhiza glabra HPLC fractionation Anti-MDR activity Ellagic acid 6-aldehydo-isoophiopogonone Liquirtigenin |
| url | http://link.springer.com/article/10.1186/s12906-018-2207-8 |
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