Migration assay on primary culture isolated from patient's primary breast cancer tissue
<p>Background: Migration is an essential component of breast cancer metastasis, which study<br />has been concentrated on culture of established breast cancer cell lines that do not accurately<br />represent the sophistication and heterogeneity of patient's breast cancer. An a...
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| Format: | Article |
| Language: | English |
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Universitas Andalas
2014-12-01
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| Series: | Majalah Kedokteran Andalas |
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| Online Access: | http://jurnalmka.fk.unand.ac.id/index.php/art/article/view/220 |
| _version_ | 1811311280785457152 |
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| author | ED Yuliana A Bustami A Bustami HP Tantry |
| author_facet | ED Yuliana A Bustami A Bustami HP Tantry |
| author_sort | ED Yuliana |
| collection | DOAJ |
| description | <p>Background: Migration is an essential component of breast cancer metastasis, which study<br />has been concentrated on culture of established breast cancer cell lines that do not accurately<br />represent the sophistication and heterogeneity of patient's breast cancer. An attempt to<br />perform migration assay using Boyden Chamber Assay (BCA) on primary culture originating<br />from patient's breast cancer tissue was developed to accommodate upcoming study of breast<br />cancer migration in lndonesian patients.<br />Methods: Pathologically proven primary breast cancer tissue samples were obtained from<br />Ciptomangunkusumo Hospital during core (n=4) and incisional (n=3) biopsies of stage llA<br />up to stage lllA breast cancer patients. Following biopsy, the breast cancer tissue samples<br />underwent processings to isolate the cancer cells. These cancer cells were -then resuspended<br />within Dulbecco's modified Eagle's medium (DMEM) ahd cultured in 12-well plate. The growth<br />of primary culture were observed and compared between the core biopsy and the incisional<br />biopsy specimens. Optimization of BCA method was later performed to investigate the<br />migration of the breast cancer primary culture towards different experirnental conditions, which<br />were control, Fetal Bovine Serum (FBS), and Stromal Derived Factor-l (SDF-1). Two different<br />number of breast cancer cells were tested for the optimization of the BCA, which were 1 x 105<br />and3x105cells.<br />Results: None of the culture performed on core biopsy specimens grew, while one out of<br />three incisional biopsy specimens grew until confluence. The one primary culture that grew<br />was later assesed using BCA to assess its migration index towards different experimental<br />conditions. Using 1 x 10s breast cancer cells in the BCA , the result of the absorbance level of<br />migrated cells showed that the migration towards SDF-1 (0.529) nearly doubled the migration<br />towards controlmedium (0.239) and FBS (0.209). Meanwhile, the absorbance levelwas simiiar<br />between the control medium (1.050), FBS (1 .103), and SDF-1 (1 .104) when the same test was<br />run with 3 x 105 breast cancer cells.<br />Discussion: Breast cancer tissue collected using incisional biopsies hao better chance to<br />grow in primary culture than those collected using core biopsies, possibly due to the difference<br />in the amount of tissue extracted. Consistent with previous research, patient's breast cancer<br />cells was shown to migrate more towards the chemokine ligand SDF-1, as compared to control<br />medium and FBS. The number of breast cancer cells run in the BCA also affected the result of<br />the migration assay, where 1 x 10s cells showed a superiority as compared to 3 x 105 cells in<br />showing the migration difference between the different experimental conditions.<br />MKA, Volume 37, Nomor.Supl. 2, November 2014<br />ED Yulian dkk<br />"<br />Migration assay on primary culture isolated from patient's primary breast cancer tissue<br />Conclusions: This study encourages and facilitates future research to study migration the intrinsic characteristic of individualized patient's breast cancer as well as to assess the change in migration characteristic of breast cancer cells after an in vivo treatment in human.<br /><br /></p> |
| first_indexed | 2024-04-13T10:16:05Z |
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| institution | Directory Open Access Journal |
| issn | 0126-2092 2442-5230 |
| language | English |
| last_indexed | 2024-04-13T10:16:05Z |
| publishDate | 2014-12-01 |
| publisher | Universitas Andalas |
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| series | Majalah Kedokteran Andalas |
| spelling | doaj.art-30216f4fc979418e93af52223e98cc472022-12-22T02:50:43ZengUniversitas AndalasMajalah Kedokteran Andalas0126-20922442-52302014-12-01370828310.22338/mka.v37i7.220218Migration assay on primary culture isolated from patient's primary breast cancer tissueED YulianaA Bustami A BustamiHP Tantry<p>Background: Migration is an essential component of breast cancer metastasis, which study<br />has been concentrated on culture of established breast cancer cell lines that do not accurately<br />represent the sophistication and heterogeneity of patient's breast cancer. An attempt to<br />perform migration assay using Boyden Chamber Assay (BCA) on primary culture originating<br />from patient's breast cancer tissue was developed to accommodate upcoming study of breast<br />cancer migration in lndonesian patients.<br />Methods: Pathologically proven primary breast cancer tissue samples were obtained from<br />Ciptomangunkusumo Hospital during core (n=4) and incisional (n=3) biopsies of stage llA<br />up to stage lllA breast cancer patients. Following biopsy, the breast cancer tissue samples<br />underwent processings to isolate the cancer cells. These cancer cells were -then resuspended<br />within Dulbecco's modified Eagle's medium (DMEM) ahd cultured in 12-well plate. The growth<br />of primary culture were observed and compared between the core biopsy and the incisional<br />biopsy specimens. Optimization of BCA method was later performed to investigate the<br />migration of the breast cancer primary culture towards different experirnental conditions, which<br />were control, Fetal Bovine Serum (FBS), and Stromal Derived Factor-l (SDF-1). Two different<br />number of breast cancer cells were tested for the optimization of the BCA, which were 1 x 105<br />and3x105cells.<br />Results: None of the culture performed on core biopsy specimens grew, while one out of<br />three incisional biopsy specimens grew until confluence. The one primary culture that grew<br />was later assesed using BCA to assess its migration index towards different experimental<br />conditions. Using 1 x 10s breast cancer cells in the BCA , the result of the absorbance level of<br />migrated cells showed that the migration towards SDF-1 (0.529) nearly doubled the migration<br />towards controlmedium (0.239) and FBS (0.209). Meanwhile, the absorbance levelwas simiiar<br />between the control medium (1.050), FBS (1 .103), and SDF-1 (1 .104) when the same test was<br />run with 3 x 105 breast cancer cells.<br />Discussion: Breast cancer tissue collected using incisional biopsies hao better chance to<br />grow in primary culture than those collected using core biopsies, possibly due to the difference<br />in the amount of tissue extracted. Consistent with previous research, patient's breast cancer<br />cells was shown to migrate more towards the chemokine ligand SDF-1, as compared to control<br />medium and FBS. The number of breast cancer cells run in the BCA also affected the result of<br />the migration assay, where 1 x 10s cells showed a superiority as compared to 3 x 105 cells in<br />showing the migration difference between the different experimental conditions.<br />MKA, Volume 37, Nomor.Supl. 2, November 2014<br />ED Yulian dkk<br />"<br />Migration assay on primary culture isolated from patient's primary breast cancer tissue<br />Conclusions: This study encourages and facilitates future research to study migration the intrinsic characteristic of individualized patient's breast cancer as well as to assess the change in migration characteristic of breast cancer cells after an in vivo treatment in human.<br /><br /></p>http://jurnalmka.fk.unand.ac.id/index.php/art/article/view/220breast cancerprimary culturemigrationBoyden chamberAssaysDF_1 B3 |
| spellingShingle | ED Yuliana A Bustami A Bustami HP Tantry Migration assay on primary culture isolated from patient's primary breast cancer tissue Majalah Kedokteran Andalas breast cancer primary culture migration Boyden chamberAssay sDF_1 B3 |
| title | Migration assay on primary culture isolated from patient's primary breast cancer tissue |
| title_full | Migration assay on primary culture isolated from patient's primary breast cancer tissue |
| title_fullStr | Migration assay on primary culture isolated from patient's primary breast cancer tissue |
| title_full_unstemmed | Migration assay on primary culture isolated from patient's primary breast cancer tissue |
| title_short | Migration assay on primary culture isolated from patient's primary breast cancer tissue |
| title_sort | migration assay on primary culture isolated from patient s primary breast cancer tissue |
| topic | breast cancer primary culture migration Boyden chamberAssay sDF_1 B3 |
| url | http://jurnalmka.fk.unand.ac.id/index.php/art/article/view/220 |
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