The Toxicity, Sublethal Effects, and Biochemical Mechanism of β-Asarone, a Potential Plant-Derived Insecticide, against <i>Bemisia tabaci</i>
<i>Bemisia tabaci</i> is a threat to agriculture worldwide because of its potential to cause devastating damage to various crops. β-asarone is a bioactive pesticidal chemical originating from <i>Acorus calamus</i> (or “Sweet Flag”) plants, and it displays significant lethal e...
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MDPI AG
2022-09-01
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| Series: | International Journal of Molecular Sciences |
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| author | Ran Wang Yong Fang Wunan Che Qinghe Zhang Jinda Wang Chen Luo |
| author_facet | Ran Wang Yong Fang Wunan Che Qinghe Zhang Jinda Wang Chen Luo |
| author_sort | Ran Wang |
| collection | DOAJ |
| description | <i>Bemisia tabaci</i> is a threat to agriculture worldwide because of its potential to cause devastating damage to various crops. β-asarone is a bioactive pesticidal chemical originating from <i>Acorus calamus</i> (or “Sweet Flag”) plants, and it displays significant lethal effects against insect pests. In this study, we established a baseline of susceptibility to β-asarone from China and patterns of cross-resistance to other popular insecticides. We found that all the 12 field-collected <i>B. tabaci</i> populations exhibited high susceptibility to β-asarone, and there was no cross-resistance detected for other tested insecticides. We subsequently evaluated the sublethal effects of β-asarone on physiology and biochemistry via LC<sub>25</sub> treatment (4.7 mg/L). LC<sub>25</sub> of β-asarone resulted in prolonged developmental duration and decreased survival rates in <i>B. tabaci</i> nymphs, pseudopupae, and adults. Significant reductions in oviposition duration, fecundity, and hatchability were also observed. Additionally, the metabolic enzyme activity and expression profiles of selected cytochrome P450 monooxygenase (P450) genes following the LC<sub>25</sub> treatment of β-asarone suggest that enhanced detoxification via P450s could be involved in the observed sublethal effects. These findings demonstrate the strong toxicity and significant sublethal effects of β-asarone on <i>B. tabaci</i> and suggest that the induced overexpression of P450 genes could be associated with the response to β-asarone. |
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| issn | 1661-6596 1422-0067 |
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| last_indexed | 2024-03-09T23:46:32Z |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-30218a8c2c1b4842bd83c68896067d6d2023-11-23T16:42:09ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-09-0123181046210.3390/ijms231810462The Toxicity, Sublethal Effects, and Biochemical Mechanism of β-Asarone, a Potential Plant-Derived Insecticide, against <i>Bemisia tabaci</i>Ran Wang0Yong Fang1Wunan Che2Qinghe Zhang3Jinda Wang4Chen Luo5Institute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaAgriculture Biotechnology Institute, Hunan Academy of Agricultural Sciences, Changsha 410125, ChinaDepartment of Pesticide Sciences, Shenyang Agricultural University, Shenyang 110866, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaNational Engineering Research Center of Sugarcane, Fujian Agricultural and Forestry University, Fuzhou 350002, ChinaInstitute of Plant Protection, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China<i>Bemisia tabaci</i> is a threat to agriculture worldwide because of its potential to cause devastating damage to various crops. β-asarone is a bioactive pesticidal chemical originating from <i>Acorus calamus</i> (or “Sweet Flag”) plants, and it displays significant lethal effects against insect pests. In this study, we established a baseline of susceptibility to β-asarone from China and patterns of cross-resistance to other popular insecticides. We found that all the 12 field-collected <i>B. tabaci</i> populations exhibited high susceptibility to β-asarone, and there was no cross-resistance detected for other tested insecticides. We subsequently evaluated the sublethal effects of β-asarone on physiology and biochemistry via LC<sub>25</sub> treatment (4.7 mg/L). LC<sub>25</sub> of β-asarone resulted in prolonged developmental duration and decreased survival rates in <i>B. tabaci</i> nymphs, pseudopupae, and adults. Significant reductions in oviposition duration, fecundity, and hatchability were also observed. Additionally, the metabolic enzyme activity and expression profiles of selected cytochrome P450 monooxygenase (P450) genes following the LC<sub>25</sub> treatment of β-asarone suggest that enhanced detoxification via P450s could be involved in the observed sublethal effects. These findings demonstrate the strong toxicity and significant sublethal effects of β-asarone on <i>B. tabaci</i> and suggest that the induced overexpression of P450 genes could be associated with the response to β-asarone.https://www.mdpi.com/1422-0067/23/18/10462<i>Bemisia tabaci</i>whiteflyβ-asaroneplant-derived toxinsublethal effectsmetabolic enzymes |
| spellingShingle | Ran Wang Yong Fang Wunan Che Qinghe Zhang Jinda Wang Chen Luo The Toxicity, Sublethal Effects, and Biochemical Mechanism of β-Asarone, a Potential Plant-Derived Insecticide, against <i>Bemisia tabaci</i> International Journal of Molecular Sciences <i>Bemisia tabaci</i> whitefly β-asarone plant-derived toxin sublethal effects metabolic enzymes |
| title | The Toxicity, Sublethal Effects, and Biochemical Mechanism of β-Asarone, a Potential Plant-Derived Insecticide, against <i>Bemisia tabaci</i> |
| title_full | The Toxicity, Sublethal Effects, and Biochemical Mechanism of β-Asarone, a Potential Plant-Derived Insecticide, against <i>Bemisia tabaci</i> |
| title_fullStr | The Toxicity, Sublethal Effects, and Biochemical Mechanism of β-Asarone, a Potential Plant-Derived Insecticide, against <i>Bemisia tabaci</i> |
| title_full_unstemmed | The Toxicity, Sublethal Effects, and Biochemical Mechanism of β-Asarone, a Potential Plant-Derived Insecticide, against <i>Bemisia tabaci</i> |
| title_short | The Toxicity, Sublethal Effects, and Biochemical Mechanism of β-Asarone, a Potential Plant-Derived Insecticide, against <i>Bemisia tabaci</i> |
| title_sort | toxicity sublethal effects and biochemical mechanism of β asarone a potential plant derived insecticide against i bemisia tabaci i |
| topic | <i>Bemisia tabaci</i> whitefly β-asarone plant-derived toxin sublethal effects metabolic enzymes |
| url | https://www.mdpi.com/1422-0067/23/18/10462 |
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