Effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice
Objective To study the effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice. Methods Twelve 12-week-old male APP/PS1 double transgenic mice were randomly divided into the control group (C group) and the 50 Hz magnetic field exposure group (MF group) (n...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Army Medical University
2023-02-01
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| Series: | 陆军军医大学学报 |
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| Online Access: | http://aammt.tmmu.edu.cn/Upload/rhtml/202206091.htm |
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| author | ZHANG Yanwen MA Qinlong GAO Peng DENG Ping PI Huifeng |
| author_facet | ZHANG Yanwen MA Qinlong GAO Peng DENG Ping PI Huifeng |
| author_sort | ZHANG Yanwen |
| collection | DOAJ |
| description | Objective To study the effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice. Methods Twelve 12-week-old male APP/PS1 double transgenic mice were randomly divided into the control group (C group) and the 50 Hz magnetic field exposure group (MF group) (n=6). The mice in the MF group were exposed to 1.0 mT 50 Hz magnetic field 2 h/d for 3 weeks (21 d). The mice in the sham exposure group had the same conditions as those in the MF group, except for no magnetic field exposure. After exposure, the cerebral cortex tissues of mice were taken according to the standard method for high-resolution untargeted metabonomics tests, including sample metabolite extraction, sample LC-MS/MS mass spectrometry analysis, metabolite qualitative and quantitative analysis and data analysis. Results The growth of APP/PS1 double transgenic mice were not affected after 50 Hz magnetic field exposure. The high-resolution untargeted metabonomics showed 1 068 metabolites were identified. Based on fold change analysis (FC>1.5 or FC < 0.67, P < 0.05), 22 significant changed metabolites were identified in cerebral cortex of mice after 50 Hz magnetic field exposure, and 11-keto-beta-boswellic acid was the most increased metabolite (FC=80.68), and 5-aminolevulinic acid was the most decreased metabolite (FC=0.34). Based on the variable importance in the projection (VIP) value of each variable in the orthogonal partial least-squares discriminant analysis (OPLS-DA) model (VIP>1, P < 0.05), 30 identified significant changed metabolites were screened in cerebral cortex of mice after 50 Hz magnetic field exposure. And the top 3 significant changed metabolites in VIP value were 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (VIP=19.76, FC=0.52), dehydroascorbic acid (VIP=12.71, FC=0.72) and inosine (VIP=10.64, FC=1.05). The analyses of hierarchical clustering, correlation and Kyoto Encyclopedia of Genes and Genomes (KEGG) showed that the 30 VIP significant changed metabolites may be involved in 16 KEGG pathways. L-aspartic acid, L-alanine and acetylcholine may be involved in 13, 7 and 3 pathways of the 16 KEGG pathways respectively. Conclusion The amino acid metabolism, ATP-binding cassette transporters, CoA and aminoacyl-tRNA biosynthesis, regulation of actin cytoskeleton and neuroactive ligand-receptor interaction may be impacted in cerebral cortex of APP/PS1 transgenic mice by 50 Hz magnetic field exposure.
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| first_indexed | 2024-04-10T07:03:39Z |
| format | Article |
| id | doaj.art-3022c9d3a5e6471b9a866c9e130b9a12 |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-04-10T07:03:39Z |
| publishDate | 2023-02-01 |
| publisher | Editorial Office of Journal of Army Medical University |
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| series | 陆军军医大学学报 |
| spelling | doaj.art-3022c9d3a5e6471b9a866c9e130b9a122023-02-27T10:39:24ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272023-02-0145427729010.16016/j.2097-0927.202206091Effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic miceZHANG Yanwen0 MA Qinlong1GAO Peng2 DENG Ping3 PI Huifeng4Department of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University(Third Military Medical University), Chongqing, 400038, ChinaDepartment of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University(Third Military Medical University), Chongqing, 400038, ChinaDepartment of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University(Third Military Medical University), Chongqing, 400038, ChinaDepartment of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University(Third Military Medical University), Chongqing, 400038, ChinaDepartment of Occupational Health, Key Laboratory of Electromagnetic Radiation Protection of Ministry of Education, Faculty of Military Preventive Medicine, Army Medical University(Third Military Medical University), Chongqing, 400038, ChinaObjective To study the effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice. Methods Twelve 12-week-old male APP/PS1 double transgenic mice were randomly divided into the control group (C group) and the 50 Hz magnetic field exposure group (MF group) (n=6). The mice in the MF group were exposed to 1.0 mT 50 Hz magnetic field 2 h/d for 3 weeks (21 d). The mice in the sham exposure group had the same conditions as those in the MF group, except for no magnetic field exposure. After exposure, the cerebral cortex tissues of mice were taken according to the standard method for high-resolution untargeted metabonomics tests, including sample metabolite extraction, sample LC-MS/MS mass spectrometry analysis, metabolite qualitative and quantitative analysis and data analysis. Results The growth of APP/PS1 double transgenic mice were not affected after 50 Hz magnetic field exposure. The high-resolution untargeted metabonomics showed 1 068 metabolites were identified. Based on fold change analysis (FC>1.5 or FC < 0.67, P < 0.05), 22 significant changed metabolites were identified in cerebral cortex of mice after 50 Hz magnetic field exposure, and 11-keto-beta-boswellic acid was the most increased metabolite (FC=80.68), and 5-aminolevulinic acid was the most decreased metabolite (FC=0.34). Based on the variable importance in the projection (VIP) value of each variable in the orthogonal partial least-squares discriminant analysis (OPLS-DA) model (VIP>1, P < 0.05), 30 identified significant changed metabolites were screened in cerebral cortex of mice after 50 Hz magnetic field exposure. And the top 3 significant changed metabolites in VIP value were 2-oleoyl-1-palmitoyl-sn-glycero-3-phosphocholine (VIP=19.76, FC=0.52), dehydroascorbic acid (VIP=12.71, FC=0.72) and inosine (VIP=10.64, FC=1.05). The analyses of hierarchical clustering, correlation and Kyoto Encyclopedia of Genes and Genomes (KEGG) showed that the 30 VIP significant changed metabolites may be involved in 16 KEGG pathways. L-aspartic acid, L-alanine and acetylcholine may be involved in 13, 7 and 3 pathways of the 16 KEGG pathways respectively. Conclusion The amino acid metabolism, ATP-binding cassette transporters, CoA and aminoacyl-tRNA biosynthesis, regulation of actin cytoskeleton and neuroactive ligand-receptor interaction may be impacted in cerebral cortex of APP/PS1 transgenic mice by 50 Hz magnetic field exposure. http://aammt.tmmu.edu.cn/Upload/rhtml/202206091.htm50 hz magnetic fieldapp/ps1 transgenic micecerebral cortexuntargeted metabolomics |
| spellingShingle | ZHANG Yanwen MA Qinlong GAO Peng DENG Ping PI Huifeng Effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice 陆军军医大学学报 50 hz magnetic field app/ps1 transgenic mice cerebral cortex untargeted metabolomics |
| title | Effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice |
| title_full | Effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice |
| title_fullStr | Effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice |
| title_full_unstemmed | Effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice |
| title_short | Effects of 50 Hz magnetic field exposure on metabolism of cerebral cortex in APP/PS1 transgenic mice |
| title_sort | effects of 50 hz magnetic field exposure on metabolism of cerebral cortex in app ps1 transgenic mice |
| topic | 50 hz magnetic field app/ps1 transgenic mice cerebral cortex untargeted metabolomics |
| url | http://aammt.tmmu.edu.cn/Upload/rhtml/202206091.htm |
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