Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases
Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST. A single use, disposable biosensor prototype, composed of catalytic iridium nano-particles dispersed on carbon paste, was developed to...
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| Format: | Article |
| Language: | English |
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MDPI AG
2012-05-01
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| Series: | Biosensors |
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| Online Access: | http://www.mdpi.com/2079-6374/2/2/234 |
| _version_ | 1811192952975785984 |
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| author | Chung-Chiun Liu Liming Dai Joanne H. Wang Chang-Jung Hsueh |
| author_facet | Chung-Chiun Liu Liming Dai Joanne H. Wang Chang-Jung Hsueh |
| author_sort | Chung-Chiun Liu |
| collection | DOAJ |
| description | Aspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST. A single use, disposable biosensor prototype, composed of catalytic iridium nano-particles dispersed on carbon paste, was developed to detect enzymatically-produced H2O2 in AST-mediated reactions. This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0–250 UL−1 specific activity). The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum. The measurements of AST in human serum using the biosensor compared well with those measured by standard hospital spectrophotometric assays. This Ir-C biosensor may be useful for AST measurements in the clinical environment. |
| first_indexed | 2024-04-12T00:00:18Z |
| format | Article |
| id | doaj.art-302537d480ec4e4983313cb360b6cd8c |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-04-12T00:00:18Z |
| publishDate | 2012-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-302537d480ec4e4983313cb360b6cd8c2022-12-22T03:56:15ZengMDPI AGBiosensors2079-63742012-05-012223424410.3390/bios2020234Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver DiseasesChung-Chiun LiuLiming DaiJoanne H. WangChang-Jung HsuehAspartate aminotransaminase (AST) is a hepatocelluar enzyme released into the bloodstream when hepatic cells are damaged, resulting in elevated blood levels of AST. A single use, disposable biosensor prototype, composed of catalytic iridium nano-particles dispersed on carbon paste, was developed to detect enzymatically-produced H2O2 in AST-mediated reactions. This biosensor is capable of measuring AST levels in a phosphate buffer and undiluted human serum over the concentration range of 0 to 0.89 μg/mL AST concentration (corresponding to 0–250 UL−1 specific activity). The biosensor operates at relatively low oxidation potential (+0.3 volt (V) versus the printed Ag/AgCl), minimizing any potential chemical interference in human serum. The measurements of AST in human serum using the biosensor compared well with those measured by standard hospital spectrophotometric assays. This Ir-C biosensor may be useful for AST measurements in the clinical environment.http://www.mdpi.com/2079-6374/2/2/234liver diseasesaspartate aminotransferaseelectrochemical detectionamperometric biosensor |
| spellingShingle | Chung-Chiun Liu Liming Dai Joanne H. Wang Chang-Jung Hsueh Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases Biosensors liver diseases aspartate aminotransferase electrochemical detection amperometric biosensor |
| title | Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases |
| title_full | Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases |
| title_fullStr | Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases |
| title_full_unstemmed | Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases |
| title_short | Development of an Electrochemical-Based Aspartate Aminotransferase Nanoparticle Ir-C Biosensor for Screening of Liver Diseases |
| title_sort | development of an electrochemical based aspartate aminotransferase nanoparticle ir c biosensor for screening of liver diseases |
| topic | liver diseases aspartate aminotransferase electrochemical detection amperometric biosensor |
| url | http://www.mdpi.com/2079-6374/2/2/234 |
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