The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, <i>Manis javanica</i>
This article describes the semen characteristics from different collection methods between captive and confiscated Malayan pangolins, <i>Manis javanica</i>. Semen was collected from 15 pangolins; two captive and 13 confiscated individuals at the mean weight of 9.36 ± 1.94 kg. The three s...
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MDPI AG
2020-10-01
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| Online Access: | https://www.mdpi.com/2076-2615/10/11/1948 |
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| author | Reza Tarmizi Yap Keng Chee Symphorosa Sipangkui Zainal Zahari Zainuddin Wan-Nor Fitri |
| author_facet | Reza Tarmizi Yap Keng Chee Symphorosa Sipangkui Zainal Zahari Zainuddin Wan-Nor Fitri |
| author_sort | Reza Tarmizi |
| collection | DOAJ |
| description | This article describes the semen characteristics from different collection methods between captive and confiscated Malayan pangolins, <i>Manis javanica</i>. Semen was collected from 15 pangolins; two captive and 13 confiscated individuals at the mean weight of 9.36 ± 1.94 kg. The three semen collection methods employed were electroejaculation, rectal massage and a combination of both techniques. The semen characteristics (mean ± standard deviation) of the Malayan pangolin are volume (73.75 ± 144.57 µL), pH (7.63 ± 0.53), spermatozoa concentration (997.19 ± 728.98 × 10<sup>6</sup> /mL), total motility (59.60% ± 30.00%), progressive motility (48.95% ± 30.93%), mass motility (3.50 ± 1.50) and live spermatozoa (80.25% ± 13.45%). There was no significant difference in semen characteristics between the three collection methods. The percentages of live spermatozoa were significantly different, suggesting better samples from captive compared to confiscated animals. However, there was no significant difference in spermatozoa kinetics between the captive and confiscated samples, suggesting the potential of utilizing confiscated individuals for gamete recovery to conserve the genetic pool of pangolins. All three methods of semen collection were successfully performed in pangolins and should be considered; however, electroejaculation remains the most consistent method of obtaining semen from the species. |
| first_indexed | 2024-03-10T15:23:24Z |
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| id | doaj.art-30260ac3455344a5b36cf225e3b95185 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-10T15:23:24Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-30260ac3455344a5b36cf225e3b951852023-11-20T18:12:48ZengMDPI AGAnimals2076-26152020-10-011011194810.3390/ani10111948The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, <i>Manis javanica</i>Reza Tarmizi0Yap Keng Chee1Symphorosa Sipangkui2Zainal Zahari Zainuddin3Wan-Nor Fitri4Borneo Rhino Alliance, Sabah, Malaysia, c/o Faculty of Sciences and Natural Resources, Universiti Malaysia Sabah, Kota Kinabalu 88400, Sabah, MalaysiaBorneo Rhino Alliance, Sabah, Malaysia, c/o Faculty of Sciences and Natural Resources, Universiti Malaysia Sabah, Kota Kinabalu 88400, Sabah, MalaysiaSabah Wildlife Department, Tingkat 4, Blok B, Wisma MUIS, Kota Kinabalu 88100, Sabah, MalaysiaBorneo Rhino Alliance, Sabah, Malaysia, c/o Faculty of Sciences and Natural Resources, Universiti Malaysia Sabah, Kota Kinabalu 88400, Sabah, MalaysiaTheriogenology and Cytogenetics Laboratory, Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia (UPM), Seri Kembangan 43400, Selangor, MalaysiaThis article describes the semen characteristics from different collection methods between captive and confiscated Malayan pangolins, <i>Manis javanica</i>. Semen was collected from 15 pangolins; two captive and 13 confiscated individuals at the mean weight of 9.36 ± 1.94 kg. The three semen collection methods employed were electroejaculation, rectal massage and a combination of both techniques. The semen characteristics (mean ± standard deviation) of the Malayan pangolin are volume (73.75 ± 144.57 µL), pH (7.63 ± 0.53), spermatozoa concentration (997.19 ± 728.98 × 10<sup>6</sup> /mL), total motility (59.60% ± 30.00%), progressive motility (48.95% ± 30.93%), mass motility (3.50 ± 1.50) and live spermatozoa (80.25% ± 13.45%). There was no significant difference in semen characteristics between the three collection methods. The percentages of live spermatozoa were significantly different, suggesting better samples from captive compared to confiscated animals. However, there was no significant difference in spermatozoa kinetics between the captive and confiscated samples, suggesting the potential of utilizing confiscated individuals for gamete recovery to conserve the genetic pool of pangolins. All three methods of semen collection were successfully performed in pangolins and should be considered; however, electroejaculation remains the most consistent method of obtaining semen from the species.https://www.mdpi.com/2076-2615/10/11/1948biobankwildlifeillegal wildlife tradeassisted reproductive technology |
| spellingShingle | Reza Tarmizi Yap Keng Chee Symphorosa Sipangkui Zainal Zahari Zainuddin Wan-Nor Fitri The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, <i>Manis javanica</i> Animals biobank wildlife illegal wildlife trade assisted reproductive technology |
| title | The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, <i>Manis javanica</i> |
| title_full | The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, <i>Manis javanica</i> |
| title_fullStr | The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, <i>Manis javanica</i> |
| title_full_unstemmed | The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, <i>Manis javanica</i> |
| title_short | The Comparison of Semen Collection in Electroejaculation, Rectal Massage and Combination of Both Methods in the Critically Endangered Malayan Pangolin, <i>Manis javanica</i> |
| title_sort | comparison of semen collection in electroejaculation rectal massage and combination of both methods in the critically endangered malayan pangolin i manis javanica i |
| topic | biobank wildlife illegal wildlife trade assisted reproductive technology |
| url | https://www.mdpi.com/2076-2615/10/11/1948 |
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