Immobilization of BoPAL3 Phenylalanine Ammonia-Lyase on Electrospun Nanofibrous Membranes of Polyvinyl Alcohol/Nylon 6/Chitosan Crosslinked with Dextran Polyaldehyde
Phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) is common in plants and catalyzes the formation of <i>trans</i>-cinnamic acid and ammonia via phenylalanine deamination. Recombinant <i>Bambusa oldhamii</i> BoPAL3 protein expressed in <i>Escherichia coli</i> was immo...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/18/3699 |
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| author | Chun-Yen Hsieh Pei-Yu Hong Lu-Sheng Hsieh |
| author_facet | Chun-Yen Hsieh Pei-Yu Hong Lu-Sheng Hsieh |
| author_sort | Chun-Yen Hsieh |
| collection | DOAJ |
| description | Phenylalanine ammonia-lyase (PAL, EC 4.3.1.24) is common in plants and catalyzes the formation of <i>trans</i>-cinnamic acid and ammonia via phenylalanine deamination. Recombinant <i>Bambusa oldhamii</i> BoPAL3 protein expressed in <i>Escherichia coli</i> was immobilized on an electrospun nanofibrous membrane using dextran polyaldehyde as a crosslinker. The immobilized BoPAL3 protein exhibited comparable kinetic properties with the free BoPAL3 protein and could be recycled for six consecutive cycles compared with the free BoPAL3 protein. The residual activity of the immobilized BoPAL3 protein was 84% after 30 days of storage at 4 °C, whereas the free BoPAL3 protein retained 56% residual activity in the same storage conditions. Furthermore, the resistance of the immobilized BoPAL3 protein to chemical denaturants was greatly increased. Therefore, the BoPAL3 protein can be immobilized using the natural dextran polyaldehyde crosslinker in place of the conventional chemical crosslinker. Nanofibrous membranes made from polyvinyl alcohol (PVA), nylon 6, and chitosan (CS) are incredibly stable and useful for future industrial applications. |
| first_indexed | 2024-03-10T22:12:08Z |
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| id | doaj.art-3026c7a1b1f54f2d81317e6205f2b38a |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T22:12:08Z |
| publishDate | 2023-09-01 |
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| record_format | Article |
| series | Polymers |
| spelling | doaj.art-3026c7a1b1f54f2d81317e6205f2b38a2023-11-19T12:34:32ZengMDPI AGPolymers2073-43602023-09-011518369910.3390/polym15183699Immobilization of BoPAL3 Phenylalanine Ammonia-Lyase on Electrospun Nanofibrous Membranes of Polyvinyl Alcohol/Nylon 6/Chitosan Crosslinked with Dextran PolyaldehydeChun-Yen Hsieh0Pei-Yu Hong1Lu-Sheng Hsieh2Department of Pathology and Laboratory Medicine, Shin Kong Wu Ho-Su Memorial Hospital, Taipei City 111, TaiwanDepartment of Food Science, College of Agriculture and Health, Tunghai University, Taichung 40704, TaiwanDepartment of Food Science, College of Agriculture and Health, Tunghai University, Taichung 40704, TaiwanPhenylalanine ammonia-lyase (PAL, EC 4.3.1.24) is common in plants and catalyzes the formation of <i>trans</i>-cinnamic acid and ammonia via phenylalanine deamination. Recombinant <i>Bambusa oldhamii</i> BoPAL3 protein expressed in <i>Escherichia coli</i> was immobilized on an electrospun nanofibrous membrane using dextran polyaldehyde as a crosslinker. The immobilized BoPAL3 protein exhibited comparable kinetic properties with the free BoPAL3 protein and could be recycled for six consecutive cycles compared with the free BoPAL3 protein. The residual activity of the immobilized BoPAL3 protein was 84% after 30 days of storage at 4 °C, whereas the free BoPAL3 protein retained 56% residual activity in the same storage conditions. Furthermore, the resistance of the immobilized BoPAL3 protein to chemical denaturants was greatly increased. Therefore, the BoPAL3 protein can be immobilized using the natural dextran polyaldehyde crosslinker in place of the conventional chemical crosslinker. Nanofibrous membranes made from polyvinyl alcohol (PVA), nylon 6, and chitosan (CS) are incredibly stable and useful for future industrial applications.https://www.mdpi.com/2073-4360/15/18/3699<i>Bambusa oldhamii</i>chitosan (CS)dextran polyaldehydeelectrospun nanofiberenzyme kineticsnylon 6 |
| spellingShingle | Chun-Yen Hsieh Pei-Yu Hong Lu-Sheng Hsieh Immobilization of BoPAL3 Phenylalanine Ammonia-Lyase on Electrospun Nanofibrous Membranes of Polyvinyl Alcohol/Nylon 6/Chitosan Crosslinked with Dextran Polyaldehyde Polymers <i>Bambusa oldhamii</i> chitosan (CS) dextran polyaldehyde electrospun nanofiber enzyme kinetics nylon 6 |
| title | Immobilization of BoPAL3 Phenylalanine Ammonia-Lyase on Electrospun Nanofibrous Membranes of Polyvinyl Alcohol/Nylon 6/Chitosan Crosslinked with Dextran Polyaldehyde |
| title_full | Immobilization of BoPAL3 Phenylalanine Ammonia-Lyase on Electrospun Nanofibrous Membranes of Polyvinyl Alcohol/Nylon 6/Chitosan Crosslinked with Dextran Polyaldehyde |
| title_fullStr | Immobilization of BoPAL3 Phenylalanine Ammonia-Lyase on Electrospun Nanofibrous Membranes of Polyvinyl Alcohol/Nylon 6/Chitosan Crosslinked with Dextran Polyaldehyde |
| title_full_unstemmed | Immobilization of BoPAL3 Phenylalanine Ammonia-Lyase on Electrospun Nanofibrous Membranes of Polyvinyl Alcohol/Nylon 6/Chitosan Crosslinked with Dextran Polyaldehyde |
| title_short | Immobilization of BoPAL3 Phenylalanine Ammonia-Lyase on Electrospun Nanofibrous Membranes of Polyvinyl Alcohol/Nylon 6/Chitosan Crosslinked with Dextran Polyaldehyde |
| title_sort | immobilization of bopal3 phenylalanine ammonia lyase on electrospun nanofibrous membranes of polyvinyl alcohol nylon 6 chitosan crosslinked with dextran polyaldehyde |
| topic | <i>Bambusa oldhamii</i> chitosan (CS) dextran polyaldehyde electrospun nanofiber enzyme kinetics nylon 6 |
| url | https://www.mdpi.com/2073-4360/15/18/3699 |
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