Characterization of human induced pluripotent stem cells line JLUEYEi002-A from a 48 year old healthy male
Peripheral blood was extracted from a 48-year old healthy male donor. Induced pluripotent stem cells (iPSC) were reprogrammed by sendai virus encoding Klf-4, c-Myc, Oct-4, and Sox-2. The iPSC line showed pluripotency, which was verified by immunofluorescence staining. The iPSC line showed normal kar...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2021-05-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506121000672 |
| _version_ | 1818950500418060288 |
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| author | Hong Wu Yan Cheng Zhenshan Lv Lidi Liu Weiquan Gong Shaokun Zhang |
| author_facet | Hong Wu Yan Cheng Zhenshan Lv Lidi Liu Weiquan Gong Shaokun Zhang |
| author_sort | Hong Wu |
| collection | DOAJ |
| description | Peripheral blood was extracted from a 48-year old healthy male donor. Induced pluripotent stem cells (iPSC) were reprogrammed by sendai virus encoding Klf-4, c-Myc, Oct-4, and Sox-2. The iPSC line showed pluripotency, which was verified by immunofluorescence staining. The iPSC line showed normal karyotype, and could form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo. This cell line can be served as healthy control for studying inherited disease. |
| first_indexed | 2024-12-20T09:19:35Z |
| format | Article |
| id | doaj.art-302b160e308d4647afc799b09f062516 |
| institution | Directory Open Access Journal |
| issn | 1873-5061 |
| language | English |
| last_indexed | 2024-12-20T09:19:35Z |
| publishDate | 2021-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj.art-302b160e308d4647afc799b09f0625162022-12-21T19:45:19ZengElsevierStem Cell Research1873-50612021-05-0153102221Characterization of human induced pluripotent stem cells line JLUEYEi002-A from a 48 year old healthy maleHong Wu0Yan Cheng1Zhenshan Lv2Lidi Liu3Weiquan Gong4Shaokun Zhang5Eye Center of 2nd Hospital, Jilin University, People's Republic of ChinaEye Center of 2nd Hospital, Jilin University, People's Republic of ChinaThe Department of Spine Surgery, 1st Hospital, Jilin University, Jilin Engineering Research Center for Spine and Spinal Cord Injury, People's Republic of ChinaThe Department of Spine Surgery, 1st Hospital, Jilin University, Jilin Engineering Research Center for Spine and Spinal Cord Injury, People's Republic of ChinaThe Department of Spine Surgery, 1st Hospital, Jilin University, Jilin Engineering Research Center for Spine and Spinal Cord Injury, People's Republic of ChinaThe Department of Spine Surgery, 1st Hospital, Jilin University, Jilin Engineering Research Center for Spine and Spinal Cord Injury, People's Republic of China; Corresponding author.Peripheral blood was extracted from a 48-year old healthy male donor. Induced pluripotent stem cells (iPSC) were reprogrammed by sendai virus encoding Klf-4, c-Myc, Oct-4, and Sox-2. The iPSC line showed pluripotency, which was verified by immunofluorescence staining. The iPSC line showed normal karyotype, and could form embryoid bodies in vitro and differentiate into the 3 germ layers in vivo. This cell line can be served as healthy control for studying inherited disease.http://www.sciencedirect.com/science/article/pii/S1873506121000672 |
| spellingShingle | Hong Wu Yan Cheng Zhenshan Lv Lidi Liu Weiquan Gong Shaokun Zhang Characterization of human induced pluripotent stem cells line JLUEYEi002-A from a 48 year old healthy male Stem Cell Research |
| title | Characterization of human induced pluripotent stem cells line JLUEYEi002-A from a 48 year old healthy male |
| title_full | Characterization of human induced pluripotent stem cells line JLUEYEi002-A from a 48 year old healthy male |
| title_fullStr | Characterization of human induced pluripotent stem cells line JLUEYEi002-A from a 48 year old healthy male |
| title_full_unstemmed | Characterization of human induced pluripotent stem cells line JLUEYEi002-A from a 48 year old healthy male |
| title_short | Characterization of human induced pluripotent stem cells line JLUEYEi002-A from a 48 year old healthy male |
| title_sort | characterization of human induced pluripotent stem cells line jlueyei002 a from a 48 year old healthy male |
| url | http://www.sciencedirect.com/science/article/pii/S1873506121000672 |
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