Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patients
IntroductionSerum autoantibodies targeting the SSA/Ro proteins are a key component of the classification criteria for the diagnosis of Sjögren’s syndrome (SS). Most patients' serum reacts with both Ro60 and Ro52 proteins. Here we compare the molecular and clinical characteristics of patients di...
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Frontiers Media S.A.
2023-03-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1115548/full |
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| author | Adrian Y. S. Lee Adrian Y. S. Lee Trishni Putty Trishni Putty Ming-Wei Lin Ming-Wei Lin Sanjay Swaminathan Sanjay Swaminathan Dan Suan Dan Suan Tim Chataway Rogier M. Thurlings Tom P. Gordon Tom P. Gordon Jing Jing Wang Jing Jing Wang Joanne H. Reed |
| author_facet | Adrian Y. S. Lee Adrian Y. S. Lee Trishni Putty Trishni Putty Ming-Wei Lin Ming-Wei Lin Sanjay Swaminathan Sanjay Swaminathan Dan Suan Dan Suan Tim Chataway Rogier M. Thurlings Tom P. Gordon Tom P. Gordon Jing Jing Wang Jing Jing Wang Joanne H. Reed |
| author_sort | Adrian Y. S. Lee |
| collection | DOAJ |
| description | IntroductionSerum autoantibodies targeting the SSA/Ro proteins are a key component of the classification criteria for the diagnosis of Sjögren’s syndrome (SS). Most patients' serum reacts with both Ro60 and Ro52 proteins. Here we compare the molecular and clinical characteristics of patients diagnosed with SS with anti-Ro52 in the presence or absence of anti-Ro60/La autoantibodies.MethodsA cross-sectional study was performed. Patients in the SS biobank at Westmead Hospital (Sydney, Australia) that were positive for anti-Ro52 were included and stratified based on the absence (isolated) or presence (combined) of anti-Ro60/La, measured by line immunoassay. We examined clinical associations and the serological and molecular characteristics of anti-Ro52 using ELISA and mass spectrometry in serological groups.ResultsA total of 123 SS patients were included for study. SS patients with isolated anti-Ro52 (12%) identified a severe serological subset characterised by higher disease activity, vasculitis, pulmonary involvement, rheumatoid factor (RhF) and cryoglobulinaemia. Serum antibodies reacting with Ro52 in the isolated anti-Ro52 subset displayed less isotype switching, less immunoglobulin variable region subfamily usage and a lower degree of somatic hypermutation than the combined anti-Ro52 subset.ConclusionsIn our cohort of SS patients, isolated anti-Ro52 represents a severe subset of SS, and is associated with the presence of cryoglobulinaemia. We therefore provide clinical relevance to the stratification of SS patients by their sero-reactivities. It is possible that the autoantibody patterns may be immunological epiphenomena of the underlying disease process, and further work is required to unearth the mechanisms of the differential clinical phenotypes. |
| first_indexed | 2024-04-10T00:13:00Z |
| format | Article |
| id | doaj.art-30337c3a00a4442cb3f1ee2d2e38b839 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-04-10T00:13:00Z |
| publishDate | 2023-03-01 |
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| series | Frontiers in Immunology |
| spelling | doaj.art-30337c3a00a4442cb3f1ee2d2e38b8392023-03-16T07:05:57ZengFrontiers Media S.A.Frontiers in Immunology1664-32242023-03-011410.3389/fimmu.2023.11155481115548Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patientsAdrian Y. S. Lee0Adrian Y. S. Lee1Trishni Putty2Trishni Putty3Ming-Wei Lin4Ming-Wei Lin5Sanjay Swaminathan6Sanjay Swaminathan7Dan Suan8Dan Suan9Tim Chataway10Rogier M. Thurlings11Tom P. Gordon12Tom P. Gordon13Jing Jing Wang14Jing Jing Wang15Joanne H. Reed16Centre for Immunology and Allergy Research, Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW, AustraliaDepartment of Clinical Immunology and Allergy, Westmead Hospital and Institute of Clinical Pathology & Medical Research (ICPMR), Westmead, NSW, AustraliaDepartment of Immunology, Flinders University, Bedford Park, SA, AustraliaDepartment of Immunology, SA Pathology, Flinders Medical Centre, Bedford Park, SA, AustraliaCentre for Immunology and Allergy Research, Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW, AustraliaDepartment of Clinical Immunology and Allergy, Westmead Hospital and Institute of Clinical Pathology & Medical Research (ICPMR), Westmead, NSW, AustraliaCentre for Immunology and Allergy Research, Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW, AustraliaDepartment of Clinical Immunology and Allergy, Westmead Hospital and Institute of Clinical Pathology & Medical Research (ICPMR), Westmead, NSW, AustraliaCentre for Immunology and Allergy Research, Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW, AustraliaDepartment of Clinical Immunology and Allergy, Westmead Hospital and Institute of Clinical Pathology & Medical Research (ICPMR), Westmead, NSW, AustraliaFlinders Proteomic Facility, Flinders University, Bedford Park, SA, AustraliaDepartment of Rheumatology, Radboud University Medical Center, Nijmegen, NetherlandsDepartment of Immunology, Flinders University, Bedford Park, SA, AustraliaDepartment of Immunology, SA Pathology, Flinders Medical Centre, Bedford Park, SA, AustraliaDepartment of Immunology, Flinders University, Bedford Park, SA, AustraliaDepartment of Immunology, SA Pathology, Flinders Medical Centre, Bedford Park, SA, AustraliaCentre for Immunology and Allergy Research, Westmead Institute for Medical Research, The University of Sydney, Westmead, NSW, AustraliaIntroductionSerum autoantibodies targeting the SSA/Ro proteins are a key component of the classification criteria for the diagnosis of Sjögren’s syndrome (SS). Most patients' serum reacts with both Ro60 and Ro52 proteins. Here we compare the molecular and clinical characteristics of patients diagnosed with SS with anti-Ro52 in the presence or absence of anti-Ro60/La autoantibodies.MethodsA cross-sectional study was performed. Patients in the SS biobank at Westmead Hospital (Sydney, Australia) that were positive for anti-Ro52 were included and stratified based on the absence (isolated) or presence (combined) of anti-Ro60/La, measured by line immunoassay. We examined clinical associations and the serological and molecular characteristics of anti-Ro52 using ELISA and mass spectrometry in serological groups.ResultsA total of 123 SS patients were included for study. SS patients with isolated anti-Ro52 (12%) identified a severe serological subset characterised by higher disease activity, vasculitis, pulmonary involvement, rheumatoid factor (RhF) and cryoglobulinaemia. Serum antibodies reacting with Ro52 in the isolated anti-Ro52 subset displayed less isotype switching, less immunoglobulin variable region subfamily usage and a lower degree of somatic hypermutation than the combined anti-Ro52 subset.ConclusionsIn our cohort of SS patients, isolated anti-Ro52 represents a severe subset of SS, and is associated with the presence of cryoglobulinaemia. We therefore provide clinical relevance to the stratification of SS patients by their sero-reactivities. It is possible that the autoantibody patterns may be immunological epiphenomena of the underlying disease process, and further work is required to unearth the mechanisms of the differential clinical phenotypes.https://www.frontiersin.org/articles/10.3389/fimmu.2023.1115548/fullanti-Ro52/TRIM21autoantibodiescryoglobulinaemiarheumatoid factor, Ro/LaSjögren’s syndrome |
| spellingShingle | Adrian Y. S. Lee Adrian Y. S. Lee Trishni Putty Trishni Putty Ming-Wei Lin Ming-Wei Lin Sanjay Swaminathan Sanjay Swaminathan Dan Suan Dan Suan Tim Chataway Rogier M. Thurlings Tom P. Gordon Tom P. Gordon Jing Jing Wang Jing Jing Wang Joanne H. Reed Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patients Frontiers in Immunology anti-Ro52/TRIM21 autoantibodies cryoglobulinaemia rheumatoid factor, Ro/La Sjögren’s syndrome |
| title | Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patients |
| title_full | Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patients |
| title_fullStr | Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patients |
| title_full_unstemmed | Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patients |
| title_short | Isolated anti-Ro52 identifies a severe subset of Sjögren’s syndrome patients |
| title_sort | isolated anti ro52 identifies a severe subset of sjogren s syndrome patients |
| topic | anti-Ro52/TRIM21 autoantibodies cryoglobulinaemia rheumatoid factor, Ro/La Sjögren’s syndrome |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2023.1115548/full |
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